V8
Full name: Endoproteinase Glu-C,from Staphylococcus aureus strain V8
Alias name: V8 Protease
CAS Number: 66676-43-5
EC Number: 3.4.21.19
DESCRIPTIONS
V8 is serine protease, specifically hydrolyzes aspartic acid and glutamic acid residue carboxyl side peptide and ester bond. The Optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are diisopropyl fluorine phosphoric acid (DFP) and alpha 2 - giant globulin and N α = - P - toluene sulfonyl - l-lysine chlorine methyl ketone (TLCK).
Unit definition: 37 ℃, pH 7.8 per minute, and catalytic hydrolysis 1 μ mol N - tertiary butyl oxygen carbonyl - L - glutamic acid alpha benzene ester enzyme quantity for a enzyme activity unit.
Specific activity: approx. 20 U/mg at 25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approx. 500 U/mg at 37°C with casein as the substrate) Contents: Liquid
specification: 50 µg per vial. Application: Protein structure and sequence analysis. Storage: -20°C.
Shanghai Yaxin Biotechnology Co. Ltd.
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