中文名：蛋白內(nèi)切酶 Glu-C 來源于金黃色葡萄球菌 V8

英文名：Endoproteinase Glu-C from Staphylococcus aureus V8

中文別名：V8蛋白酶

純度：≥500 units/mg dry weight

貨號：P128655

包裝：1mg、5mg

Cas號：66676-43-5

存儲溫度：2-8°C儲存

產(chǎn)品介紹：

Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping .

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