Recombinant lipase A
Biocatalyst with excellent enantioselectivity
SOURCE:
E.Coli
MOL. WEIGHT:
65 kDa
FORM:
Lyophilized powder, Immobilized powder
DESCRIPTIONS
Lipases (EC 3.1.1.3) are ubiquitous enzymes that catalyze the hydrolysis of fats and oils, playing important roles in pharmaceutical. The lipase from Serratia marcescens (SML) is well-known for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-(4-methoxyphynyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM], a key intermediate for the synthesis of diltiazem hydrochloride.
APPLICATION
1)Hydrolyze trans-3-(4-methoxyphynyl) glycidicacid methyl ester [(±)-MPGM] to produce (2R,3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM].
2)Hydrolyze (±)-naproxen methyl ester to produce(-)-naproxen.
3)Hydrolyze Fatty acid esters.
4)Catalyze turn ester reaction.
5)Produce biodiesel.
APPLICATION NOTES
Be stable below 40°C, unstable at higher than 50°C.
Stability against organic solvents
In 10%-50% DMSO, isopropyl ether, petroleum ether
In 10%-25% ethanol, acetone and isopropanol,
In 90% isopropyl ether, petroleum ether
In 50% acetone and isopropanol.
Selectivity to different fatty-acids derives
Priory to long-chain (> 10) fatty acids derives.
APPLICATION EXAMPLES
Figure 1. HPLC figure of (±)-MPGM using toluence as solvent,lipase A as catalyst. A, (±)-MPGM mixture before reaction; B,adding lipase A for 4h.
Figure 2. HPLC analysis on lipase A catalize (±)-naproxen methy ester with chiral selectivity
Figure 3. Selectivity of lipase A on different long-chain fatty acids p-nitrophenyl derives.
PRODUCTS INFORMATION
UNIT DEFINITION :Lipase was assayed using p-nitrophenyl acetate ( pNPA) as a substrate.
One unit of lipase activity was defined as the amount of enzyme releasing
1.0 μmol of p-nitrophenol per minute.
關(guān)鍵字: Recombinant lipase A
Shanghai Yaxin Biotechnology Co., Ltd. is a biotech company founded in 2008 that focuses on the research and the production of recombinant proteins.
Our main products are Animal Origin Free recombinant carboxypeptidase B and recombinant trypsin, which are of high importance for the production of human recombinant insulin.