(1)非抗體柱純化,產(chǎn)品中無(wú)任何其它抗體如IgG等的污染。 (2)pH耐受范圍廣,可耐受0.5 M NaOH和0.5M HCl 處理,不降解,抗體結(jié)合能力不變。 (3)產(chǎn)品有特為抗體純化柱設(shè)計(jì)的連接基團(tuán),可直接用于蛋白A柱的制備。 (4)具有生產(chǎn)規(guī)模大,質(zhì)量穩(wěn)定,純度高等優(yōu)點(diǎn)。 重組蛋白A可偶聯(lián)到固體分離介質(zhì)(如agarose)上,用于單克隆抗體或多克隆抗體的純化。重組蛋白A可與多種報(bào)告分子(熒光染料、酶標(biāo)記、生物素、膠體金、放射性標(biāo)記等)偶聯(lián)并不影響其與抗體結(jié)合的活性。這些偶聯(lián)后的蛋白A衍生物可用于Western-blot、ELISA、免疫組化等的抗體檢測(cè)。
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains.Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b,IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonaland polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
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