Anti-CD3 epsilon/CD3e Antibody (PerCP), Mouse MonoclonalMouseHumanRecombinant Human CD3e / CD3 epsilon proteinFCMThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3e / CD3 epsilon  and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed.

Anti-CD3 epsilon/CD3e Antibody (PerCP), Mouse Monoclonal---Sino Biological

Flow cytometric analysis of Human CD3 expression on human peripheral blood lymphocytes. Cells were stained with PerCP-conjugated anti-Human CD3 and APC conjugated anti-Human CD19 (BD Pharmingen™ Cat. No. 555415). The dot plots were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature na?ve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.在免疫學(xué)中，CD3（分化簇3）T細(xì)胞的共受體是一種蛋白質(zhì)復(fù)合物，它由四個不同的鏈組成。在哺乳動物中，該復(fù)合物含有一個CD3γ鏈，CD3δ鏈，和2 CD3ε鏈。這些鏈具有被稱為一個分子副T細(xì)胞受體（TCR）和ζ-鏈以產(chǎn)生激活信號的T淋巴細(xì)胞。該TCR，ζ鏈和CD3分子一起構(gòu)成的T細(xì)胞受體復(fù)合物。 CD3分子是T細(xì)胞的重要標(biāo)志，由γ、δ、ε、ζ和η五種多肽鏈組成，它們的胞漿區(qū)均含有ITAM，具有傳導(dǎo)TCR信號的功能，使T細(xì)胞活化。CD3分子與TCR以非共價鍵結(jié)合形成TCR-CD3復(fù)合物，其主要功能是把TCR與抗原結(jié)合后產(chǎn)生的活化信號傳遞到細(xì)胞內(nèi)，誘導(dǎo)T細(xì)胞活化。PERCP標(biāo)記小鼠抗人CD3單克隆抗體是一類可以特異性結(jié)合CD3的單克隆抗體，主要用于檢測CD3的Western Blot、IHC-P、IF、ELISA、Co-IP等多種免疫學(xué)實(shí)驗(yàn)。雙抗體夾心法測定標(biāo)本中CD3水平。用純化的CD3抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入CD3，再與HRP標(biāo)記的CD3抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的CD3呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中CD3濃度。