1239-45-8
中文名稱
溴化乙啶
英文名稱
Ethidium bromide
CAS
1239-45-8
EINECS 編號
214-984-6
分子式
C21H20BrN3
MDL 編號
MFCD00011724
分子量
394.31
MOL 文件
1239-45-8.mol
更新日期
2024/12/12 14:08:18
1239-45-8 結(jié)構(gòu)式
基本信息
中文別名
溴化-3,8-二氨基-5-乙基-6-苯基菲啶翁溴化乙啶
溴化乙錠
溴化乙錠(EB)
溴乙菲啶
2,7-二氨基-9-苯基氮雜菲-10-乙基溴
3,8-二氨基-5-乙基-6-苯基菲啶溴化物
菲啶溴紅
溴化-3,8-二氨基-5-乙基-6-苯基菲啶
2,7-二氨基-9-苯基氮雜菲-10-乙基溴,菲啶溴紅,溴化-3,8-二氨基-5-乙基-6-苯菲啶
溴乙錠(EB)
菲啶溴紅,1生物技術(shù)級98+%
菲啶溴紅溶液
菲啶溴紅,10MG片劑
菲啶溴紅,100MG片劑
溴化乙啶/溴化乙錠
溴化乙腚
胡米溴銨
溴化乙錠, 98%(DRY WT.)
英文別名
1,7-DIAMINO-10-ETHYL-9-PHENYLPHENANTHRIDINIUM BROMIDE2,7-DIAMINO-10-ETHYL-9-PHENYLPHENANTHRIDINIUM BROMIDE
2,7-DIAMINO-10-ETHYL-9-PHENYLPHENANTHRIDIUM BROMIDE
3,8-DIAMINO-5-ETHYL-6-PHENYLPHENANTHRIDINIUM BROMIDE
3,8-DIAMINO-5-ETHYL-6-PHENYLPHENANTHRIDIUM BROMIDE
3,8-DIAMINO-6-ETHYL-5-PHENYL-PHENANTHRIDIUM BROMIDE
5-ETHYL-3,8-DIAMINO-6-PHENYL-PHENANTHRIDINIUM BROMIDE
CA NUMBERING: 3,8-DIAMINO-5-ETHYL-6-PHENYL-PHENANTHRIDINIUM BROMIDE
ETBR
ETHIDIUM BROMIDE
HOMIDIUM BROMIDE
2,7-Diamino-9-phenyl-10-ethylphenanthridiniumbromide
2,7-Diamino-9-phenylphenanthridineethobromide
3,8-diamino-5-ethyl-6-phenyl-phenanthridiniubromide
Dromilac
Phenanthridinium,3,8-diamino-5-ethyl-6-phenyl-,bromide
Rd1572
Ethidium bromide(EB)
Ethidium bromide solution
3,8-diamino-1-ethyl-6-phenylphenantridinium bromide
所屬類別
生物化工：生物染料物理化學(xué)性質(zhì)
外觀性質(zhì)為芳香族熒光化合物。熔點(diǎn):260 - 262℃
熔點(diǎn)260-262 °C (dec.)(lit.)
密度1.3739 (rough estimate)
折射率1.6700 (estimate)
閃點(diǎn)>100°C
儲存條件2-8°C
溶解度H2O: 10 mg/mL, opaque, strongly red
形態(tài)powder
形態(tài)粉末
顏色紅色至深紫色
氣味 (Odor)無味固體
水溶解性40 g/L (25 ºC)
最大波長(λmax)518 nm, 210 nm, 285 nm, 316 nm,
343 nm, 480 nm, 525 nm
Merck14,4731
BRN3642536
穩(wěn)定性穩(wěn)定的。與強(qiáng)氧化劑不相容。
生物領(lǐng)域應(yīng)用Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification
主要應(yīng)用Electroluminescent displays;photoresists
CAS 數(shù)據(jù)庫1239-45-8(CAS DataBase Reference)
EPA化學(xué)物質(zhì)信息Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1239-45-8)
安全數(shù)據(jù)
警示詞危險(xiǎn)
危險(xiǎn)性描述H331-H341
危險(xiǎn)品標(biāo)志T,T+
危險(xiǎn)類別碼R23-R68-R36/37/38-R26-R21/22
危險(xiǎn)品運(yùn)輸編號UN 2811 6.1/PG 1
WGK Germany3
RTECS號SF7950000
F8-9
危險(xiǎn)等級6.1
包裝類別I
海關(guān)編碼29339990
毒害物質(zhì)數(shù)據(jù)1239-45-8(Hazardous Substances Data)
毒性Intercalating dye widely used to stain
DNA in gels and gradients. The DNA can be visualized readily
by irradiation with ultraviolet light, as little as 0.5 μg of DNA
being detectable by such methods. Ethidium bromide is also
added to cesium chloride density gradients, since as an intercalating
molecule it binds more readily to linear DNA than to
closed collinear circles of DNA (such as plasmids). Binding of
ethidium bromide reduces the density of DNA, thus covalent
circles of DNA have higher densities at saturating concentrations
of ethidium bromide, permitting the separation of plasmid
DNA. Ethidium bromide is a putative carcinogen.
應(yīng)用領(lǐng)域
用途一
EB不能穿過活細(xì)胞膜,可通過死細(xì)胞破損膜對細(xì)胞核DNA染色,含一個(gè)可嵌入堆積堿基之間的一平面基團(tuán),該基團(tuán)固定位置及與堿基密切接近,致染料與DNA結(jié)合現(xiàn)熒光,因EB-DNA復(fù)合物熒光產(chǎn)率遠(yuǎn)大于未結(jié)合染料熒光,當(dāng)電泳凝膠中含游離EB,可測少量DNA,是高靈敏熒光試劑,也是重要熒光染料,常測DNARNA.與DNA結(jié)合抑制DNA復(fù)制轉(zhuǎn)錄用途二
溴化乙錠是一種高度靈敏的熒光染色劑，用于觀察瓊脂糖和聚丙烯酰胺凝膠中的DNA。溴化乙錠用標(biāo)準(zhǔn)302nm 紫外光透射儀激發(fā)并放射出橙紅色信號，可用Polaroid 底片或帶CCD 成像頭的凝膠成像處理系統(tǒng)拍攝。常見問題列表
熒光探針技術(shù)中的熒光探劑
溴化乙啶(簡稱EB)是目前在紫外線下觀察DNA而常用的一種熒光染料，屬于插入染料，原因是它有多環(huán)結(jié)構(gòu)，這種結(jié)構(gòu)使它能夠插入核苷酸堿基之間。由于核酸和堿基的熒光量子產(chǎn)率很低，因此利用天然熒光檢測核酸比蛋白質(zhì)困難更大，所以熒光探針技術(shù)在核酸研究中顯得尤為重要。比較常用的是溴化乙啶(et-hidium bromide或稱菲啶溴紅)，它與核酸 (DNA、RNA、雙鏈多聚核苷酸)有特異結(jié)合的能力。它對脫氧核糖核酸的最低檢測量可達(dá)10ng/ml，而且它與核酸的雙鏈區(qū)結(jié)合是專一的，利用它與各種構(gòu)象核酸結(jié)合比例的不同所產(chǎn)生的熒光強(qiáng)度的變化，可以把各種構(gòu)型核酸加以區(qū)分。例如它能鑒別天然核酸和變性核酸，區(qū)分線性DNA、環(huán)狀DNA和超級線團(tuán)DNA等。此外各種吖啶，包括吖啶橙、吖啶黃以及前黃素等都是核酸常用的探劑。熒光分析法可分為直接測定法和間接測定法二種。直接測定法是利用物質(zhì)自身發(fā)射的熒光，即所謂“自熒光”或“內(nèi)源熒光”來進(jìn)行測定。但在自然界中只有少部分物質(zhì)在光激發(fā)后可以發(fā)射熒光，而有相當(dāng)一部分物質(zhì)發(fā)熒光很弱或不發(fā)射熒光，如蛋白和核酸就是發(fā)熒光很弱的物質(zhì)。為了充分利用熒光分析法靈敏度高這一特點(diǎn)就需使其轉(zhuǎn)化成熒光物質(zhì)再進(jìn)行測定。此時(shí)可用某些試劑(如熒光染料) 使其與熒光較弱或不顯熒光的物質(zhì)共價(jià)或非共價(jià)結(jié)合，以形成發(fā)熒光的結(jié)合物再進(jìn)行測定，這就是所謂的“熒光探針”技術(shù)或“熒光標(biāo)記”技術(shù)。使試劑(熒光劑) 吸附在大分子上或共價(jià)結(jié)合到大分子上。所用的染料溴化乙啶被叫做“熒光探劑”。探劑熒光特性的變化，實(shí)際上是由于大分子構(gòu)象的變化所引起的探劑環(huán)境變化的信號，所以利用這種信號還可以得到大分子構(gòu)象變化的信息。
生物活性
Ethidium Bromide (Homidium bromide, EtBr, EB) 是一種嵌入劑，與DNA堿基對類似。EtBr在分子生物學(xué)實(shí)驗(yàn)技術(shù)（如瓊脂糖凝膠電泳）中經(jīng)常被用于熒光標(biāo)記（核酸染色）。體外研究
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The preparation of agarose gel:
1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.
2. Mix the agarose powder with 1X TAE/TBE.
3. Microwave for 1-3 min until the agarose is completely dissolved (
Caution
: not overboil).
4. Make the solution cool down before solidification.
5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 μL stock/100 mL gel).
6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.
CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
知名試劑公司產(chǎn)品信息
Acros Organics
溴化乙啶Ethidium bromide, pure, 95%(1239-45-8)
Sigma Aldrich
1239-45-8(sigmaaldrich)TCI Shanghai
溴化乙啶Ethidium Bromide,>90.0%(T)(1239-45-8)
溴化乙啶價(jià)格(試劑級)
| 報(bào)價(jià)日期 | 產(chǎn)品編號 | 產(chǎn)品名稱 | CAS號 | 包裝 | 價(jià)格 |
| 2024/11/11 | XW12394583 | 溴化乙錠 ethidium bromide;3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide;5-ethyl-3,8-diamino-6-phenylphenanthridinium bromide;homidium bromide | 1239-45-8 | 25G | 2068元 |
| 2024/11/11 | XW12394582 | 溴化乙錠 ethidium bromide;3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide;5-ethyl-3,8-diamino-6-phenylphenanthridinium bromide;homidium bromide | 1239-45-8 | 5G | 476元 |
| 2024/11/11 | XW12394581 | 溴化乙錠 ethidium bromide;3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide;5-ethyl-3,8-diamino-6-phenylphenanthridinium bromide;homidium bromide | 1239-45-8 | 1G | 142元 |