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Phytochemical Analysis

Phytochemical Analysis

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HPLC Fingerprint Combined With Chemometrics and Multicomponent Content Determination for Quality Evaluation and Control of Huangma Tincture

Published:10 December 2024 DOI: 10.1002/pca.3487 PMID: 39658907
Jing Qiu,?Jie Li,?Si-Yang Shang,?Peng Zhou,?Jing Leng

Abstract

Introduction: Huangma Tincture (HMT) is a Chinese patent medicine with a history of clinical use for more than 60?years, widely used for treating dermal chronic ulcer such as diabetic foot ulcer. However, the overall quality evaluation and control method of HMT has not yet been researched.

Objective: The aim of this study is to establish a comprehensive quality evaluation and control method for HMT based on high-performance liquid chromatography (HPLC) fingerprint, chemometrics, and multicomponent content determination.

Methods: Establishing chemical fingerprint of HMT and carrying out similarity analysis comprehensively reflect the consistency of the formulation in terms of chemical composition. Chemometrics analyses, including hierarchical cluster analysis (HCA), principal component analysis (PCA), and orthogonal partial least squares-discriminant analysis (OPLS-DA), were performed to identify components crucial to quality differences. The content of five potential bioactive ingredients (brucine, strychnine, jatrorrhizine, coptisine, and berberine hydrochloride) was determined as a supplementary quality control measure.

Results: HPLC fingerprint of HMT with similarity index > 0.990 was established, in which five common compounds (brucine, strychnine, jatrorrhizine, coptisine, and berberine hydrochloride) were identified. HCA, PCA, and OPLS-DA results, validated through 200 permutation tests, were basically consistent. The contents of brucine, strychnine, jatrorrhizine, coptisine, and berberine hydrochloride in 20 batches of HMT samples were 0.2088-0.5556, 0.2599-0.9868, 0.1358-0.2092, 0.2634-0.6843, and 1.8301-2.7826?mg/mL, respectively.

Conclusion: HPLC fingerprint combined with chemometrics and multicomponent content determination considering both effect and toxicity provides a robust method for the comprehensive quality evaluation and control of HMT.

Substances (5)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Acetonitrile 75-05-8 C2H3N 1028 suppliers $61180.00
Methanol 67-56-1 CH4O 837 suppliers $9.00-$7620.00
Berberine hydrochloride 633-65-8 C20H18NO4.Cl 682 suppliers $5.00-$650.00
2,9,10-Trimethoxy-5,6-dihydroisoquinolino[2,1-b]isoquinolin-7-ium-3-ol 3621-38-3 C20H20INO4 184 suppliers $17.00-$2541.50
COPTISINE 3486-66-6 C19H14NO4+ 152 suppliers $69.00-$700.00

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