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RNAi-Ready pSIREN-RetroQ-DsRed-Express 載體,RNAi-Ready pSIREN-RetroQ-DsRed-Express
  • RNAi-Ready pSIREN-RetroQ-DsRed-Express 載體,RNAi-Ready pSIREN-RetroQ-DsRed-Express

RNAi-Ready pSIREN-RetroQ-DsRed-Express 載體

價格 1280
包裝 2ug
最小起訂量 2ug
發(fā)貨地 上海
更新日期 2024-11-15
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產(chǎn)品詳情

中文名稱:RNAi-Ready pSIREN-RetroQ-DsRed-Express 載體英文名稱:RNAi-Ready pSIREN-RetroQ-DsRed-Express
保存條件: 常溫運(yùn)輸純度規(guī)格: 99%
產(chǎn)品類別: 質(zhì)粒/載體
2024-11-15 RNAi-Ready pSIREN-RetroQ-DsRed-Express 載體 RNAi-Ready pSIREN-RetroQ-DsRed-Express 2ug/1280RMB 1280 常溫運(yùn)輸 99% 質(zhì)粒/載體

RNAi-Ready pSIREN-RetroQ-DsRed-Express 載體

質(zhì)粒類型:
RNAi載體
高拷貝/低拷貝:
低拷貝
啟動子:
Human U6
克隆方法:
多克隆位點(diǎn),限制性內(nèi)切酶
載體大小:
6543 bp (查看載體序列)
5' 測序引物及序列:
U6-F: ATGGACTATCATATGCTTACCGTA (Clontech )
載體抗性:
Ampicillin (氨芐青霉素)
備注:
載體表達(dá)DsRed紅色熒光蛋白作為基因沉默標(biāo)志。
產(chǎn)品編號
產(chǎn)品名稱
規(guī)格
價格
ZY1402
RNAi-Ready pSIREN-RetroQ-DsRed-Express
2ug
 
RNAi-Ready pSIREN-RetroQ-DsRed-Express 質(zhì)粒圖譜
RNAi-Ready pSIREN-RetroQ-DsRed-Express Vector Map

載體說明

RNAi-Ready pSIREN-RetroQ-DsRed-Express is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter (PU6; RNA Pol III-dependent). RNAi-Ready pSIREN-RetroQ-DsRed-Express is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when a ds DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQDsRed- Express construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA.

This vector also expresses a variant of Discosoma sp. red fluorescent protein (DsRed-Express; 1), which has been engineered for improved solubility (excitation maximum = 557 nm; emission maximum = 579 nm). The DsRed-Express gene is positioned just downstream of the immediate early promoter of cytomegalovirus (PCMV IE). As a result, cells transfected with this vector will express the red fluorescent protein constitutively. The DsRed-Express fluorescent marker allows you to directly monitor the delivery efficiency of your gene silencing construct using either fluorescence microscopy or flow cytometry 8–12 hours after transfection.

This retroviral vector is optimized to eliminate promoter interference through self-inactivation. The hybrid 5' LTR consists of the cytomegalovirus (CMV) type I enhancer and the mouse sarcoma virus (MSV) promoter. This construct drives high levels of transcription in HEK 293-based packaging cell lines due, in part, to the presence of adenoviral E1A (2–5) in these cells. The self-inactivating feature of the vector is provided by a deletion in the 3' LTR enhancer region (U3). During reverse transcription of the retroviral RNA, the inactivated 3' LTR is copied and replaces the 5' LTR, resulting in inactivation of the 5' LTR CMV enhancer sequences. This may reduce the phenomenon known as promoter interference (6) and allow for more efficient expression.

Also included in the viral genomic transcript are the necessary viral RNA processing elements including the LTRs, packaging signal (Psi+), and tRNA primer binding site. RNAi-Ready pSIREN-RetroQ-DsRed-Express also contains a bacterial origin of replication and E. coli Ampr gene for propagation and selection in bacteria.

載體應(yīng)用

RNAi-Ready pSIREN-RetroQ-DsRed-Express is used for targeted gene silencing when a ds DNA oligonucleotide encoding an appropriate shRNA is inserted. To construct recombinant pSIREN-RetroQ-DsRed-Express, first design, generate, and anneal complementary shRNA oligonucleotides using the protocols in the Knockout RNAi Systems User Manual (PT3739-1). The annealed oligonucleotide should contain 5'-BamH I and 3'-EcoR I overhangs. Then ligate the annealed oligonucleotide into RNAi-Ready pSIREN-RetroQ-DsRed-Express.

Your pSIREN-RetroQ-DsRed-Express construct can be transfected as a plasmid expression vector to screen for functional shRNA oligonucleotides. For gene silencing experiments using viral delivery, transfect the pSIREN-RetroQDsRed- Express construct into a packaging cell line (see the Retroviral Gene Transfer and Expression User Manual, PT3132-1, for a list of packaging cell lines available from Clontech); RNA from the vector is packaged into infectious retroviral particles. These retroviral particles can infect a wide range of target cells and transmit the shRNA but cannot replicate within these cells due to the absence of viral structural genes.

The DsRed-Express fluorescent marker in this vector allows direct monitoring of the delivery of your gene silencing construct. Use fluorescence microscopy or flow cytometry to easily detect or enrich for cells containing your recombinant shRNA vector.

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關(guān)鍵字: RNAi-Ready pSIREN-RetroQ-DsRed-Express 載體/RNAi-Ready pSIREN-RetroQ-DsRed-Express質(zhì)粒

公司簡介

上海澤葉生物科技有限公司是一家服務(wù)于生命科學(xué)領(lǐng)域的高新技術(shù)企業(yè),主要為科研機(jī)構(gòu)、高校、院所及企業(yè)產(chǎn)品開發(fā)研究提供所需要的科研類試劑、耗材、儀器、技術(shù)服務(wù)等。包括分子生物學(xué)、免疫學(xué)、微生物學(xué)、細(xì)胞學(xué)、材料科學(xué),通用試劑、藥物合成試劑、手性化合物、催化劑及配體、分析試劑、生物試劑,檢測試劑等等
成立日期 2016-08-08 (9年) 注冊資本 100萬元整
員工人數(shù) 1-10人 年營業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 生物化工,有機(jī)原料,化學(xué)試劑,醫(yī)藥原料,技術(shù)服務(wù) 經(jīng)營模式 貿(mào)易,試劑,定制,服務(wù)
  • 上海澤葉生物科技有限公司
VIP 5年
  • 公司成立:9年
  • 注冊資本:100萬元整
  • 企業(yè)類型:貿(mào)易
  • 主營產(chǎn)品:主營產(chǎn)品:ELISA試劑盒、重組蛋白、抗體、生化試劑、標(biāo)準(zhǔn)品、分子生物學(xué)試劑、細(xì)胞生物學(xué)等科研用品
  • 公司地址:松江區(qū)新橋鎮(zhèn)賣新公里2077號8309室
詢盤

RNAi-Ready pSIREN-RetroQ-DsRed-Express 載體相關(guān)廠家報價

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