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RNAi-Ready pSIREN-RetroQ 載體,RNAi-Ready pSIREN-RetroQ
  • RNAi-Ready pSIREN-RetroQ 載體,RNAi-Ready pSIREN-RetroQ

RNAi-Ready pSIREN-RetroQ 載體

價(jià)格 1000
包裝 2ug
最小起訂量 2ug
發(fā)貨地 上海
更新日期 2024-11-15
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產(chǎn)品詳情

中文名稱:RNAi-Ready pSIREN-RetroQ 載體英文名稱:RNAi-Ready pSIREN-RetroQ
保存條件: 常溫運(yùn)輸純度規(guī)格: 99%
產(chǎn)品類別: 質(zhì)粒/載體
2024-11-15 RNAi-Ready pSIREN-RetroQ 載體 RNAi-Ready pSIREN-RetroQ 2ug/1000RMB 1000 常溫運(yùn)輸 99% 質(zhì)粒/載體

RNAi-Ready pSIREN-RetroQ 載體

質(zhì)粒類型:
RNAi載體
高拷貝/低拷貝:
低拷貝
啟動(dòng)子:
Human U6
克隆方法:
多克隆位點(diǎn),限制性內(nèi)切酶
載體大小:
6455 bp (查看載體序列)
5' 測(cè)序引物及序列:
U6-F: ATGGACTATCATATGCTTACCGTA (Clontech )
載體抗性:
Ampicillin (氨芐青霉素)
篩選標(biāo)記:
Puromycin (嘌呤霉素)
產(chǎn)品編號(hào)
產(chǎn)品名稱
規(guī)格
價(jià)格
ZY1401
RNAi-Ready pSIREN-RetroQ
2ug
 
RNAi-Ready pSIREN-RetroQ 質(zhì)粒圖譜

RNAi-Ready pSIREN-RetroQ is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter (PU6; RNA Pol III-dependent). RNAi-Ready pSIREN-RetroQ is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an oligonuceotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQ construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA. The vector contains a puromycin resistance gene for the selection of stable transfectants.

This retroviral vector is optimized to eliminate promoter interference through self-inactivation. The hybrid 5' LTR consists of the cytomegalovirus (CMV) type I enhancer and the mouse sarcoma virus (MSV) promoter. This construct drives high levels of transcription in HEK 293-based packaging cell lines due, in part, to the presence of adenoviral E1A (1–4) in these cells. The self-inactivating feature of the vector is provided by a deletion in the 3' LTR enhancer region (U3). During reverse transcription of the retroviral RNA, the inactivated 3' LTR is copied and replaces the 5' LTR, resulting in inactivation of the 5' LTR CMV enhancer sequences. This may reduce the phenomenon known as promoter interference (5) and allow more efficient expression.

Also included in the viral genomic transcript are the necessary viral RNA processing elements including the LTRs, packaging signal (Psi+), and tRNA primer binding site. RNAi-Ready pSIREN-RetroQ also contains a bacterial origin of replication and E. coli Ampr gene for propagation and selection in bacteria.

載體應(yīng)用

RNAi-Ready pSIREN-RetroQ is used for targeted gene silencing when an oligonuceotide encoding an appropriate shRNA is inserted. To construct recombinant pSIREN-RetroQ, first design, generate, and anneal complementary shRNA oligonucleotides using the protocols in the Knockout RNAi Systems User Manual (PT3739-1). The annealed oligonucleotide should contain 5'-BamH I and 3'-EcoR I sites. Then ligate the annealed oligonucleotide into RNAi-Ready pSIREN-RetroQ.

Your pSIREN-RetroQ construct can be transfected as a plasmid expression vector to screen for functional shRNA oligonucleotides. For gene silencing experiments using viral delivery, transfect the pSIREN-RetroQ construct into a packaging cell line (such as the RetroPack™ PT67 Cell Line (Cat. No. 631510), AmphoPack™-293 Cell Line, EcoPack2™-293 Cell Line, or Pantropic System). RNA from the vector is packaged into infectious retroviral particles. These infectious particles are replication-incompetent since pSIREN-RetroQ lacks structural genes (gag, pol, and env) necessary for particle formation and replication; however, these genes are stably integrated as part of the packaging cell genome. These retroviral particles can infect a wide range of target cells and transmit the shRNA but cannot replicate within these cells due to the absence of viral structural genes. The separate introduction and integration of the structural genes into the packaging cell line minimizes the chances of producing replication-competent virus due to recombination events during cell proliferation.

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關(guān)鍵字: RNAi-Ready pSIREN-RetroQ 載體/RNAi-Ready pSIREN-RetroQ質(zhì)粒

公司簡(jiǎn)介

上海澤葉生物科技有限公司是一家服務(wù)于生命科學(xué)領(lǐng)域的高新技術(shù)企業(yè),主要為科研機(jī)構(gòu)、高校、院所及企業(yè)產(chǎn)品開發(fā)研究提供所需要的科研類試劑、耗材、儀器、技術(shù)服務(wù)等。包括分子生物學(xué)、免疫學(xué)、微生物學(xué)、細(xì)胞學(xué)、材料科學(xué),通用試劑、藥物合成試劑、手性化合物、催化劑及配體、分析試劑、生物試劑,檢測(cè)試劑等等
成立日期 2016-08-08 (9年) 注冊(cè)資本 100萬元整
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬以內(nèi)
主營(yíng)行業(yè) 生物化工,有機(jī)原料,化學(xué)試劑,醫(yī)藥原料,技術(shù)服務(wù) 經(jīng)營(yíng)模式 貿(mào)易,試劑,定制,服務(wù)
  • 上海澤葉生物科技有限公司
VIP 5年
  • 公司成立:9年
  • 注冊(cè)資本:100萬元整
  • 企業(yè)類型:貿(mào)易
  • 主營(yíng)產(chǎn)品:主營(yíng)產(chǎn)品:ELISA試劑盒、重組蛋白、抗體、生化試劑、標(biāo)準(zhǔn)品、分子生物學(xué)試劑、細(xì)胞生物學(xué)等科研用品
  • 公司地址:松江區(qū)新橋鎮(zhèn)賣新公里2077號(hào)8309室
詢盤

RNAi-Ready pSIREN-RetroQ 載體相關(guān)廠家報(bào)價(jià)

產(chǎn)品名稱 價(jià)格   公司名稱 報(bào)價(jià)日期
詢價(jià)
VIP6年
上海弘順生物科技有限公司
2024-11-17
¥100
VIP6年
上??道噬锟萍加邢薰?/div>
2024-11-15
詢價(jià)
明光市興欣礦業(yè)有限責(zé)任公司
2024-11-15
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