稱 曲拉通 (Triton X-100)
規(guī)格 100ML | 500ML
存儲 室溫保存
產(chǎn)品簡介 Triton X-100，中文名字曲拉通X-100，一種非離子表面活性劑（結(jié)構(gòu)類似于igepal、NP-40），在分子生物學(xué)及免疫學(xué)中用于促進(jìn)脂質(zhì)和蛋白質(zhì)的溶解，可用于多種實驗，如細(xì)胞裂解、蛋白提取、酶類儲存液以及其他蛋白穩(wěn)定液的配制、ICC/IHC等。
產(chǎn)品性質(zhì)
英文別名（English Synonym） X-100；T-octylphenoxypolyethoxyethanol
CAS號（CAS NO.） 9002-93-1
分子式（Formula） C34H62O11
分子量（Molecular Weight） 647
密度（Density） 1.057g/ml-1.065g/ml（20℃）
外觀（Appearance） 無色或淡黃色粘稠夜體。
純度（Purity） >98%
溶解性（Solubility） 能溶于水、甲苯、二甲苯、乙醇，不溶于石油醚。
注意事項
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Triton X-100 (C14H22O(C2H4O)n) is a nonionic surfactant that has a hydrophilic polyethylene oxide chain (on average it has 9.5 ethylene oxide units) and an aromatic hydrocarbon lipophilic or hydrophobic group.
Undiluted Triton X-100 is a clear viscous fluid (but less viscous than undiluted glycerol) owing to the hydrogen bonding of its hydrophilic polyethylene oxide parts. Undiluted Triton X-100 has a viscosity of about 270 centipoise at 25 °C which comes down to about 80 centipoise at 50 °C.
Triton X-100 is a commonly used detergent in laboratories. Triton X-100 is widely used to lyse cells to extract protein or organelles, or to permeabilize the membranes of living cells.
Some applications include
Industrial purpose (plating of metal)
Permeabilizing unfixed (or lightly fixed) eukaryotic cell membranes
Solubilizing membrane proteins in their native state in conjunction with zwitterionic detergents such as CHAPS
Part of the lysis buffer (usually in a 5% solution in alkaline lysis buffer) in DNA extraction
Reducing surface tension of aqueous solutions during immunostaining (usually at a concentration of 0.1-0.5% in TBS or PBS Buffer)
Dispersion of carbon materials for soft composite materials
Restricting colony expansion in Aspergillus nidulans in microbiology
Decellularization of animal-derived tissues
Removing SDS from SDS-PAGE gels prior to renaturing the proteins within the gel
Disruption of cell monolayers as a positive control for TEER measurements.
Apart from laboratory use, Triton X-100 can be found in several types of cleaning compounds, ranging from heavy-duty industrial products to gentle detergents. It is also a popular ingredient in homemade vinyl record cleaning fluids together with distilled water and isopropyl alcohol.[citation needed] It is a good micellar catalyst.