產(chǎn)品簡(jiǎn)介：

硫酸銨(Ammonium sulfate)分子量為132.14，化學(xué)式為(NH4)2·SO4，CAS號(hào)為7783-20-2，飽和硫酸銨溶液多用于硫酸銨鹽析法沉淀蛋白質(zhì)。硫酸銨鹽析法是分離蛋白質(zhì)常用的方法之一，其原理是當(dāng)高濃度的鹽加入到蛋白溶液中，銨離子和硫酸根離子同蛋白質(zhì)競(jìng)爭(zhēng)與水結(jié)合，使蛋白質(zhì)的溶解性降低而從水溶液中沉淀析出。飽和硫酸銨溶液(SAS)pH為7.0～7.2，操作簡(jiǎn)單，不易引起蛋白質(zhì)變性失活，其缺點(diǎn)是所得抗體純度不高，易受其他蛋白質(zhì)污染。

試劑保存：室溫保存，一年有效。

操作步驟(僅供參考)：按實(shí)驗(yàn)具體要求操作。

注意事項(xiàng)：為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

Ammonium sulphate precipitation is the most commonly used salt ppt because it is highly soluble in water, it stabilizes most proteins in solution (although it may denature some MAbs), and helps reduce the lipid content of the sample.

Ammonium sulphate ppt removes up to 50% of contaminating protein, and therefore reduces the load for subsequent chromatography. Most precipitated proteins retain their activity and native conformation, and can be redissolved easily. Ideal for crude and dilute samples.

TO MAKE A SOLUTION 20% IN AMMONIUM SULFATE (FROM 100% STOCK) ADD 0.25 VOLUMES OF 100% AMMONIUM SULFATE.

Here's an example: Say you have 3 mls of a solution and want to make it 20% ammonium sulfate. The final volume will be 3 mls + x mls where x is the amount of ammonium sulfate you need to add.

Using:
(Final V) x (final C) = (V of concentrate added) x (concentrate C).

Then:
(3 + x) x (20%) = (x) x (100%)
60 + 20x = 100x
60 = 80x
x = 3/4 or 0.75 ml.
so, to bring a 3 ml volume to 20% ammonium sulfate you will need to add 0.75 mls of 100% concentrate.

TO MAKE A SOLUTION 40% IN AMMONIUM SULFATE (FROM 100% STOCK) ADD 0.67 VOLUMES OF 100%% AMMONIUM SULFATE.
(3 + x) x (40%) = (x) x (100%)
120 + 40x = 100x
120 = 60x
x = 2 mls (or 0.67 volumes).

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