The Methylamp＆#8482; Methylated DNA Capture Kit is a complete set of essential components which enables the experimenter to use proprietary and unique procedure/compositions to enrich methylated DNA. In the assay, an antibody specific to methyl cytosine is used to capture methylated genomic DNA. The enriched methylated fractions can be then used for a standard DNA detection. The entire procedure can be completed within 3 hours and produces far superior results than any competitor kits. The Methylamp＆#8482; Methylated DNA Capture Kit is suitable for combining the specificity of enriched methylated DNA with qualitative and quantitative PCR, and southern blot as well as DNA microarray.

WHY CHOOSE THE METHYLAMP＆#8482; METHYLATED DNA CAPTURE KIT?

Highly efficient enrichment of methylated DNA: > 98%.
The fastest procedure available, which can be finished within 3 hours.
Strip microplate format makes the assay flexible: manual or high throughput.
Columns for DNA purification are included: save time and reduce labor.
Compatible with all DNA amplification-based approaches.
Simple, reliable, and consistent assay conditions.

Principle & Procedure
The Methylamp＆#8482; Methylated DNA Capture Kit contains all reagents required for carrying out a successful capture of methylated DNA from a DNA sample. Particularly, this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA is sheared, added into the microwell and captured by the antibody. DNA is released from the antibody-captured methylated DNA complex, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.