背景資料

The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Pim1 is involved in the control of cytokine-mediated cell proliferation， differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional， post-transcriptional， translational and post-translational. Recently， Pim1 has been shown to enhance the activities of p100， c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis.

應(yīng)用類型

Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes ~33 kDa of PIM1 isoform from samples of human， mouse and rat origins. Reactivity to other species has not been determined.

免疫原

Synthetic peptide surrounding amino acid 296 of rat PIM1

來源宿主

兔

反應(yīng)性

經(jīng)檢測驗證，可與人、小鼠、大鼠種屬來源的PIM1蛋白發(fā)生免疫反應(yīng).

保存建議

提供的PIM1抗體產(chǎn)品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5 % BSA和0.01%硫柳汞的PBS， pH 7.2）緩沖液中.請將該PIM1抗體產(chǎn)品置于-20 ℃冷凍保存，建議分裝使用以避免反復(fù)凍融.