背景資料

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases （class III HDACs） and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. This SIRT family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes including transcriptional regulation， cell cycle progression， DNA damage repair and aging. SIRT1 has been shown to be induced by caloric restriction （CR） in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR. SIRT1 also regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53.

應(yīng)用類型

Western blot: 1:500  1:2000 dilution， IHC: 1:50 - 1:200 dilution. However， the optimal conditions should be determined individually.

免疫原

C terminal peptide of human SIRT1

來源宿主

兔

反應(yīng)性

經(jīng)檢測驗證，可與人、小鼠、大鼠種屬來源的SIRT1蛋白發(fā)生免疫反應(yīng).

保存建議

提供的SIRT1抗體產(chǎn)品為無色溶液形式，含有100 ug抗體.該產(chǎn)品濃度為與批次相關(guān)，溶解于包含有50% 甘油和0.02%疊氮化鈉的PBS緩沖液（pH 7.3）中.請在收到該SIRT1抗體產(chǎn)品后，將其置于-20 °C冷凍保存，建議分裝使用以避免反復(fù)凍融.