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重組腸激酶,Recombinant Enterokinase

重組腸激酶

價(jià)格 180
包裝 100U;500U;1000U;10ku;20ku;100ku
最小起訂量 100U;500U;1000U;10ku;20ku;100ku
發(fā)貨地 上海
更新日期 2021-05-11

產(chǎn)品詳情

中文名稱:重組腸激酶英文名稱:Recombinant Enterokinase
CAS:9014-74-8有效期: 一年
品牌: YaxinBio產(chǎn)地: 上海
保存條件: 2℃-8℃純度規(guī)格: 99%
2021-05-11 重組腸激酶 Recombinant Enterokinase RMB 180 一年 YaxinBio 上海 2℃-8℃ 99%

Recombinant Enterokinase

Catalog Number:REK08

CAS:9017-74-8
EC:3.4.21.9

Storage temperature: -20°C or below

Source: Recombinant enterokinase is a genetically engineered protein expressed in E.coli 

Protein Sequence:The amino acid sequence of recombinant enterokinase is identical to bovine enterokinase light chain

 

1.DESCRIPTION

YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase light chain. The enzyme has been extensively purified by HPLC and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K). So,enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.

 

2.MAIN FEATURES

Source

Recombinant E. coli

Appearance

Clear,colorless to yellowish liquid

Activity

≥5 u/μl

Purity(SDS-PAGE) 

Single major band

Molecular Weight(SDS-PAGE)

25.8 ±2.58 kDa

UNIT DEFINITIONOne unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein. 

 

3.RECOMMEND USAGE CONDITION:

1) Cutting condition:           25mM Tris-HCl 8.0

   Fusion protein concentration:  0.1-1mg/ml (total protein content: 50-100ug)

   EK content:                   0.1-0.2U

   Temperature:                  25℃

   Time:                         overnight or 12h-16h for digestion.

   If digestion under 2-8°C,prolong the digestion time two folds,or more enzyme added to short the digestion time.

2) 4°C low temperature digestion conditions:

   4°C can effectively digest the substrate, but need to extend the digestion time to 48-64h, or increase the amount of 2-3 times the amount of enzyme.

3) Enzyme cleavage optimization and amplification

   Optimization: Enzyme cleavage conditions can be optimized, such as buffer pH, fusion protein concentration, EK amount, and digestion time, so that the fusion protein can be digested under stable conditions, SDS-PAGE detection of digestion effect.

   Amplification: Select the optimized reaction conditions, according to the conditions such as the proportion of amplification, SDS-PAGE detection of digestion effect.

4) Method for removing recombinant enterokinase: The recombinant enterokinase was removed using trypsin inhibitor affinity chromatography (e.g., sigma T0637).

5) Note: do not recommend cutting at 37°C conditions, there may be non-specific enzyme digestion.

 

4.COMMON COMPONENTS INFLUENCE THE ACTION OF ENTEROKINASE

  >200mM imidazole or >200mM NaCl or >5% glycerin, the reaction may be effected.The following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.

 

5. STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant enterokinase should be stored under -20°C in sealed container.

It is stable within 24 months.If stored at 25°C ,it is stable within one week.

It is no activity loss after 5 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

6.PRODUCT ADVANTAGE

  1. Animal origin free: Recombinant enterokinase is no exogenous virus contamination,and any animal origin material is not used in the production process.
  2. Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
  3. High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
  4. Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
  5. Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
  6. Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

 

7.RELATED PRODUCTS

Recombinant carboxypeptidase B

Sequencing grade trypsin

Recombinant human trypsin

Recombinant trypsin

關(guān)鍵字: bovine enterokinase;enterokinase;recombinant enterokinase

公司簡介

上海雅心生物技術(shù)有限公司主要從事基因工程蛋白酶的研發(fā)和生產(chǎn),目前的主要品種為基因工程羧肽酶B、基因工程胰蛋白酶和基因工程脂肪酶A;為本公司自主研發(fā),擁有自主知識(shí)產(chǎn)權(quán)。公司擁有自組研發(fā)團(tuán)隊(duì),擁有世界先進(jìn)的重組生物技術(shù);自主研發(fā)的重組生物用酶為世界上生物用酶的首創(chuàng)! 基因工程羧肽酶B,基因工程胰蛋白酶和基因工程脂肪酶A都是由基因工程方法生產(chǎn),純度高,不含雜酶,無羧肽酶A、糜蛋白酶等其他蛋白酶活性;無動(dòng)物源性,出口產(chǎn)品無需進(jìn)行終產(chǎn)品的動(dòng)物源性檢驗(yàn)。制品中不含蛋白酶抑制劑,產(chǎn)品安全。 公司秉承“科技致勝”的理念,堅(jiān)持“提供高純度重組蛋白酶”,并愿與廣大同行交流,有需求的客戶提供優(yōu)質(zhì)的服務(wù)! 產(chǎn)品經(jīng)多
成立日期 2008-11-25 (16年) 注冊(cè)資本 100.0萬人民幣
員工人數(shù) 10-50人 年?duì)I業(yè)額 ¥ 100萬-300萬
主營行業(yè) 色譜分析,質(zhì)譜,醫(yī)藥中間體,生化試劑 經(jīng)營模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 上海雅心生物技術(shù)有限公司
非會(huì)員
  • 公司成立:16年
  • 注冊(cè)資本:100.0萬人民幣
  • 企業(yè)類型:有限責(zé)任公司(自然人投資或控股)
  • 主營產(chǎn)品:重組蛋白和重組蛋白酶
  • 公司地址:上海奉賢光建路158號(hào)2號(hào)樓
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