Recombinant Protein A
Catalog Number:RSPA05
CAS:91932-65-9
Storage temperature: 2-8℃
Source:Protein A mutant of Staphylococcus aureus , expressed in E. coli
1.DESCRIPTION
Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies from different subtypes of mammals or genetically engineered recombinant proteins containing antibody Fc fragments. alkali protein A is our company independent research and development production, with independent intellectual property rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more. The purification process does not use antibody column affinity chromatography to avoid the possibility of incorporation of unrelated IgG in the product.
2.MAIN FEATURES
3.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.
After dissolved with pure water, it should be stored under -20℃.
Stability of transport: The product is stable by blue ice insulation transport.
4.PRODUCT ADVANTAGE
1) Non-antibody column purification, the product without any other antibodies such as IgG and other pollution.
2) SPA-25 is our own research and development products, pH tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment, no degradation, antibody binding capacity unchanged.
3) The product has a specially designed link for the purification of the column, can be directly used for the preparation of protein A column.
4) Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
5) High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
6) Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
7) Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
8) Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
5.APPLICATION:
The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.
6.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant human trypsin
Recombinant enterokinase
關(guān)鍵字： Protein A；recombinant protein A
上海雅心生物技術(shù)有限公司主要從事基因工程蛋白酶的研發(fā)和生產(chǎn)，目前的主要品種為基因工程羧肽酶B、基因工程胰蛋白酶和基因工程脂肪酶A；為本公司自主研發(fā)，擁有自主知識(shí)產(chǎn)權(quán)。公司擁有自組研發(fā)團(tuán)隊(duì)，擁有世界先進(jìn)的重組生物技術(shù)；自主研發(fā)的重組生物用酶為世界上生物用酶的首創(chuàng)！
基因工程羧肽酶B，基因工程胰蛋白酶和基因工程脂肪酶A都是由基因工程方法生產(chǎn)，純度高，不含雜酶，無(wú)羧肽酶A、糜蛋白酶等其他蛋白酶活性；無(wú)動(dòng)物源性，出口產(chǎn)品無(wú)需進(jìn)行終產(chǎn)品的動(dòng)物源性檢驗(yàn)。制品中不含蛋白酶抑制劑，產(chǎn)品安全。
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