異硫氰酸熒光素(FITC)廣泛用于通過(guò)胺基將熒光標(biāo)記附著到蛋白質(zhì)上。異硫氰酸酯基團(tuán)與蛋白質(zhì)中的氨基末端和伯胺反應(yīng)。它已用于標(biāo)記蛋白質(zhì),包括抗體和凝集素。
應(yīng)用
熒光素異硫氰酸酯異構(gòu)體 I已用于分生孢子的染色。 用于蛋白FITC標(biāo)記的試劑;蛋白和肽的微測(cè)序(HPLC)
Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
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