3326-32-7
中文名稱
異硫氰酸熒光素酯
英文名稱
Fluorescein isothiocyanate isomer I
CAS
3326-32-7
EINECS 編號(hào)
248-207-7
分子式
C21H11NO5S
MDL 編號(hào)
MFCD00005063
分子量
389.38
MOL 文件
3326-32-7.mol
更新日期
2025/02/21 10:08:15
3326-32-7 結(jié)構(gòu)式
基本信息
中文別名
熒光素-5-異氰酸酯異硫氰酸熒光酯異構(gòu)體
異硫氰酸熒光橙紅
異硫氰酸熒光紅
異硫氰酸熒光黃
異硫氰酸熒光素（同分異構(gòu)體I）
熒光素5-異硫氰酸鹽,異構(gòu)體1,95%
異硫氰酸熒光素異構(gòu)體1.95+%
異硫氰酸熒光素異構(gòu)體1.97%
異硫氰酸熒光酯異構(gòu)體/FITC
FITC,熒光素-5-異硫氰酸鹽異構(gòu)體I
熒光胺異硫腈酸鹽
熒光素-5-異硫氰酸鹽異構(gòu)體I
異硫氰酸熒光素酯
5-異氰硫基熒光素
異硫氰酸熒光酯異構(gòu)體I
異硫氰酸熒光素, ISOMER 1
英文別名
2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-5-ISOTHIOCYANATO-BENZOIC ACID5-FITC
5-FLUORESCEIN ISOTHIOCYANATE
5-FLUORESCEIN ISOTHIOCYANATEISOMER
5-FLUORESCEIN ISOTHIOCYANATE, ISOMER I
5-ISOTHIOCYANATOFLUORESCEIN
FITC
FITC-CELITE(R)
FITC ISOMER
FITC 'ISOMER I'
FITC, ISOMER I
FLUORESCEIN 5(6)-ISOTHIOCYANATE
FLUORESCEIN 5-ISOTHIOCYANATE
FLUORESCEIN 5-ISOTHIOCYANATE, ISOMER 1
FLUORESCEIN-5-ISOTHIOCYANATE ISOMER I
FLUORESCEIN ISOMER I ISOTHIOCYANATE
FLUORESCEIN ISOTHIOCYANATE
FLUORESCEIN ISOTHIOCYANATE, ISOMER 1
FLUORESCEIN ISOTHIOCYANATE ISOMER I
FLUORESCEIN ISOTHIOCYANATE ISOMER I-CELITE(R)
所屬類別
化學(xué)試劑：其他酯類化合物物理化學(xué)性質(zhì)
外觀性質(zhì)黃色粉末。有吸濕性。能與各種抗體蛋白結(jié)合，結(jié)合后的抗體不喪失與一定抗原結(jié)合的特異性，并在堿性溶液中仍有強(qiáng)烈綠色熒光，加酸后析出沉淀，熒光消失,微溶于丙酮、乙醚和石油醚。不溶于水。熔點(diǎn):>360 °C
熔點(diǎn)>360 °C(lit.)
熔點(diǎn)>360 °C(lit.)
沸點(diǎn)708.6±60.0 °C(Predicted)
密度1.3423 (rough estimate)
折射率1.5060 (estimate)
儲(chǔ)存條件2-8°C
儲(chǔ)存條件2-8°C
溶解度微溶于DMSO、乙醇、甲醇DMF和丙酮
酸度系數(shù)(pKa)2.2, 4.4, 6.7(at 25℃)
形態(tài)powder
顏色白色
酸堿指示劑變色ph值范圍Weak green uorescence (6.0) to strong green uorescence (7.2)
水溶解性幾乎不溶于水
敏感性濕氣敏感
最大波長(zhǎng)(λmax)490nm
BRN1407295
穩(wěn)定性穩(wěn)定，但對(duì)水分敏感。與強(qiáng)氧化劑、強(qiáng)堿、胺、酸不相容。
生物領(lǐng)域應(yīng)用Analyzing sugar
chain; detecting iodide/iodate or total iodine;
detecting/imaging thiols; detecting/quantifying biotin
derivatives;16 diagnosing classical Hodgkin lymphoma
(CHL) in lymph nodes; cellulose nanocrystals for
bioimaging applications; treating neuroinflammation;
treating/diagnosing cancer; PEO-PCL-PEO triblock
copolymers for topical delivery; polymersomes in
biomedical or cosmetic applications;apoptosis assay;
as a substrate for measuring heparanase activity, human
immunodeficiency virus (HIV-1) proteinase activity,
kinase activity, phosphatase activity, β-lactamase
activity, transglutaminase (TG2) activity; implantable
drug-delivery devicess
主要應(yīng)用Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
InChIKeyMHMNJMPURVTYEJ-UHFFFAOYSA-N
CAS 數(shù)據(jù)庫(kù)3326-32-7(CAS DataBase Reference)
存儲(chǔ)注意事項(xiàng)濕度敏感
存儲(chǔ)注意事項(xiàng)濕度敏感;光敏感
安全數(shù)據(jù)
警示詞危險(xiǎn)
危險(xiǎn)性描述H317-H334
防范說(shuō)明P261-P272-P280-P284-P302+P352-P333+P313
危險(xiǎn)品標(biāo)志Xn,Xi
危險(xiǎn)品標(biāo)志Xn
危險(xiǎn)類別碼42/43-36/37/38-22
危險(xiǎn)類別碼R42/43-R36/37/38-R22
安全說(shuō)明22-45-36/37/39-26-36/37-36
安全說(shuō)明S22-S45-S36/37/39-S26-S36/37
WGK Germany3
WGK Germany3
F10
TSCAYes
海關(guān)編碼29329990
常見問(wèn)題列表
簡(jiǎn)介
異硫氰酸熒光素酯是黃色粉末，有吸濕性。其能與各種抗體蛋白結(jié)合，結(jié)合后的抗體不喪失與一定抗原結(jié)合的特異性，并在堿性溶液中仍有強(qiáng)烈綠色熒光，加酸后析出沉淀，熒光消失，微溶于丙酮、乙醚和石油醚。
用途
異硫氰酸熒光素酯能和各種抗體蛋白結(jié)合，結(jié)合后的抗體不喪失與一定抗原結(jié)合的特異性，并在堿性溶液中具有強(qiáng)烈的黃綠色熒光。通過(guò)在熒光顯微鏡下觀察或流式細(xì)胞儀分析可對(duì)相應(yīng)抗原進(jìn)行定性、定位或定量的檢測(cè)。用于醫(yī)學(xué)，農(nóng)學(xué)和畜牧等方面，可對(duì)由細(xì)菌病毒和寄生蟲等所致疾病進(jìn)行快速診斷。生物活性
FITC (Fluorescein 5-isothiocyanate) 是一種用于胺標(biāo)記的熒光探針。FITC 是對(duì) pH 和 Cu2+ 敏感的熒光染料。體外研究
Annexin V-FITC/PI is employed to detect chondrocyte apoptosis. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites.