resDetect? Gentamicin ELISA Kit (high sensitivity)

背景(Background)
Residues of gentamicin are prone to occur in the production process of biological products, which can easily lead to abnormal reactions in the human body. Therefore, the residual amount of gentamicin in biological products or semi-finished products of biological products should be strictly controlled.
應(yīng)用說明(Application)
The kit is developed for the detection of Gentamicin in drug products or semi-manufactures.
It is for research use only.
存儲(chǔ)(Storage)
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理(Assay Principles)
The Gentamicin quantitative detection kit adopts the indirect competitive ELISA method, and the pre-coated conjugated Gentamicin antigen on the microstrip competes with the residual Gentamicin in the sample to bind the enzyme-labeled anti-Gentamicin monoclonal antibody, and then uses a microplate reader to detect the absorbance value by adding TMB substrate, and the absorbance value is negatively correlated with the content of kanamycin in the sample. The kit takes only about one hour and 20 minutes to operate and has a linear range of 0.1 ng/mL to 3.2 ng/mL.

Detection of Gentamicin by competitive ELISA Assay Immobilized Gentamicin antigen competes with the residual Gentamicin in the sample to bind the enzyme-labeled anti-Gentamicin monoclonal (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.


關(guān)鍵字: Gentamicin;Gentamicin試劑盒;慶大霉素試劑盒;ACRO;百普賽斯;
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