Product Number: CS3409
CAS Number: 3483-12-3
Appearance：White Powder
Molecular formula: C4H10O2S2
Molecular weight：154.25 g/mol
Solubility：0.1 M in H2O, 20℃, complete, colorless
Storage Temperature: 2-8℃
DTT is synthetic. A method of preparation has been described in J. Chem. Soc. 253 (1949).
DTT is an agent for reducing -S-S- to -SH. Redox potential: -0.33 volts at pH 7. DTT acts as a protective agent for -SH groups of other species in solution. In this reaction the DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. A specific and sensitive assay for disulfide groups is based on determination of resulting monothiols with DTNB.
Applications:
It is used in buffers for isolation of RNA or buffers used for RT-PCR starting with mRNA.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions:
The solubility of DTT has been tested by Fluka at 50 mg/ml in water, resulting in a clear colorless solution. It has been described as freely soluble in water, ethanol, acetone, ethylate, chloroform and ether. Solutions oxidize relatively slowly in air. It is recommended to make solutions fresh daily.
關(guān)鍵字： DTT ;二硫蘇糖醇;
金克隆生物公司位于北京經(jīng)濟(jì)技術(shù)開發(fā)區(qū)，主要從事生物醫(yī)藥試劑的研發(fā)和生產(chǎn)，并兼營部分生化試劑和儀器耗材，主營方向有細(xì)胞生物學(xué)、分子生物學(xué)、蛋白與免疫、組織學(xué)、微生物培養(yǎng)等相關(guān)試劑與耗材。庫存現(xiàn)貨10000多種，其中我公司自主開發(fā)的分子生物學(xué)試劑、細(xì)胞培養(yǎng)及檢測試劑、分子免疫學(xué)產(chǎn)品等在質(zhì)量和價(jià)格方面具有較大的市場優(yōu)勢，歡迎廣大經(jīng)銷商洽談合作。