中文名稱(chēng)

Ras 活化檢測(cè)生化試劑盒

英文名字

Ras Activation Assay Biochem Kit

供應(yīng)商

Cytoskeleton

產(chǎn)品貨號(hào)

BK008

背景資料

該Ras 活化檢測(cè)生化試劑盒產(chǎn)品是Cytoskeleton品牌原裝進(jìn)口，Cytoskeleton品牌是細(xì)胞骨架研究專(zhuān)家，專(zhuān)注于生物化學(xué)和細(xì)胞過(guò)程研究中的純化蛋白和便捷試劑盒開(kāi)發(fā)與生產(chǎn)。 

產(chǎn)品描述

收到該Ras 活化檢測(cè)生化試劑盒Ras Activation Assay Biochem Kit產(chǎn)品后，查看廠家提供的產(chǎn)品說(shuō)明書(shū)詳細(xì)介紹。

產(chǎn)品特點(diǎn)

The Ras switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of Ras-like GTPases is of obvious biological significance and is a subject of intense investigation. The fact that Ras family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Ras protein activation. The assay uses the Ras-binding domain (RBD) of the Ras effector kinase Raf1. The Raf-RBD domain has been shown to bind specifically to the GTP-bound form of Ras proteins. The fact that the Raf-RBD has a high affinity for GTP-Ras and that its binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of Ras make it an ideal tool for affinity purification of GTP-Ras from cell lysates. The Raf-RBD is in the form of a GST fusion protein, which allows one to "pull-down" the Raf-RBD/GTP-Ras complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Ras activation in cells. The amount of activated Ras is determined by a western blot using a Ras specific antibody.

保存建議

廠家推薦常溫運(yùn)輸，收到產(chǎn)品后建議保存于4°C。

其他

Cytoskeleton公司成立于1993年，專(zhuān)注于生物化學(xué)和細(xì)胞過(guò)程研究中的純化蛋白和便捷試劑盒開(kāi)發(fā)與生產(chǎn)。公司提供藥物篩選、信號(hào)轉(zhuǎn)導(dǎo)、蛋白質(zhì)轉(zhuǎn)錄后修飾（PTM）、細(xì)胞骨架研究相關(guān)的系列試劑盒和產(chǎn)品，尤其以細(xì)胞骨架相關(guān)研究見(jiàn)長(zhǎng)，既能滿足于樣品較少的科學(xué)研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動(dòng)蛋白，小G蛋白，GAPs，GEFs等現(xiàn)有產(chǎn)品的藥物篩選服務(wù)。