中文名稱
Ras 活化檢測生化試劑盒
英文名字
Ras Activation Assay Biochem Kit
供應(yīng)商
Cytoskeleton
產(chǎn)品貨號
BK008
產(chǎn)品報價
¥11049.792/KIT50assays
產(chǎn)品說明書
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購買方式
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產(chǎn)品新聞
背景資料
該Ras 活化檢測生化試劑盒產(chǎn)品是Cytoskeleton品牌原裝進(jìn)口,Cytoskeleton品牌是細(xì)胞骨架研究專家,專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。艾美捷作為中國區(qū)域的總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨(dú)立專業(yè)技術(shù)支持團(tuán)隊的高科技生物企業(yè)??梢詾槟峁┘皶r的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
收到該Ras 活化檢測生化試劑盒Ras Activation Assay Biochem Kit產(chǎn)品后,查看廠家提供的產(chǎn)品說明書詳細(xì)介紹。
產(chǎn)品特點
The Ras switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of Ras-like GTPases is of obvious biological significance and is a subject of intense investigation. The fact that Ras family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Ras protein activation. The assay uses the Ras-binding domain (RBD) of the Ras effector kinase Raf1. The Raf-RBD domain has been shown to bind specifically to the GTP-bound form of Ras proteins. The fact that the Raf-RBD has a high affinity for GTP-Ras and that its binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of Ras make it an ideal tool for affinity purification of GTP-Ras from cell lysates. The Raf-RBD is in the form of a GST fusion protein, which allows one to "pull-down" the Raf-RBD/GTP-Ras complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Ras activation in cells. The amount of activated Ras is determined by a western blot using a Ras specific antibody.
保存建議
廠家推薦常溫運(yùn)輸,收到產(chǎn)品后建議保存于4°C。
其他
Cytoskeleton公司成立于1993年,專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。公司提供藥物篩選、信號轉(zhuǎn)導(dǎo)、蛋白質(zhì)轉(zhuǎn)錄后修飾(PTM)、細(xì)胞骨架研究相關(guān)的系列試劑盒和產(chǎn)品,尤其以細(xì)胞骨架相關(guān)研究見長,既能滿足于樣品較少的科學(xué)研究,也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外,公司還提供微管蛋白,肌動蛋白,小G蛋白,GAPs,GEFs等現(xiàn)有產(chǎn)品的藥物篩選服務(wù)。
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