中文名稱

Cdc42 Pulldown 活化檢測試劑盒

英文名字

Cdc42 Pulldown Activation Assay Kit

供應商

Cytoskeleton

產品貨號

BK034-S

背景資料

該Cdc42 Pulldown 活化檢測試劑盒產品是Cytoskeleton品牌原裝進口，Cytoskeleton品牌是細胞骨架研究專家，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產。 

產品描述

收到該Cdc42 Pulldown 活化檢測試劑盒Cdc42 Pulldown Activation Assay Kit產品后，查看廠家提供的產品說明書詳細介紹。

產品特點

The Rho switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of the GTPases is of obvious biological significance and is a subject of intense investigation. The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Rac and Cdc42 protein activation. The assay uses the Cdc42/Rac Interactive Binding (CRIB) region (also called the p21 Binding Domain, PBD) of the Cdc42 / Rac effector protein, p21 activated kinase 1 (PAK). The CRIB/PBD protein motif has been shown to bind specifically to the GTP-bound form of Rac and/or Cdc42 proteins. The fact that the PBD region of PAK has a high affinity for both GTP-Rac and GTP-Cdc42 and that PAK binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of both Rac and Cdc42 make it an ideal tool for affinity purification of GTP-Rac and GTP-Cdc42 from cell lysates. The PAK-PBD protein supplied in this kit corresponds to residues 67-150. This includes the highly conserved CRIB region (aa 74-88) plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42. The PAK-PBD is in the form of a GST fusion protein, which allows one to "pull-down" the PAK-PBD/GTP-Cdc42 (or GTP-Rac) complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Rac/Cdc42 activation in cells. The amount of activated Cdc42 is determined by a Western blot using a Cdc42 specific antibody.

保存建議

廠家推薦常溫運輸，收到產品后建議保存于4°C。

其他

Cytoskeleton公司成立于1993年，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產。公司提供藥物篩選、信號轉導、蛋白質轉錄后修飾（PTM）、細胞骨架研究相關的系列試劑盒和產品，尤其以細胞骨架相關研究見長，既能滿足于樣品較少的科學研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動蛋白，小G蛋白，GAPs，GEFs等現有產品的藥物篩選服務。