兔多克隆抗體to IL-6Rabbit與反應(yīng): HumanUnconjugatedProduced in rabbits immunized with purified, recombinant Human IL6 / IL-6 (rh IL6 / IL-6; Catalog#10395-HNAE; NP_000591.1; Val30-Met212). IL6 / IL-6 specific IgG was purified by Human IL6 / IL-6 affinity chromatography.適用于: WB, IHC-P

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody (ab6672)

IHC image of IL6 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6672, 1/400, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 13 (gp13) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.雙抗體夾心法測定標(biāo)本中HUMAN IL-6水平。用純化的HUMAN IL-6抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入HUMAN IL-6，再與HRP標(biāo)記的HUMAN IL-6抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的HUMAN IL-6呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中HUMAN IL-6濃度。
ANTI-HUMAN IL-6是一類可以特異性結(jié)合HUMAN IL-6的多克隆抗體，主要用于檢測HUMAN IL-6的Western Blot、IHC-P、IF、ELISA、Co-IP等多種免疫學(xué)實驗。

白細(xì)胞介素-6(Interleukin-6)，簡稱白介素6(IL-6)，是一種功能廣泛的多效性細(xì)胞因子。IL-6可調(diào)節(jié)多種細(xì)胞的生長與分化，具有調(diào)節(jié)免疫應(yīng)答、急性期反應(yīng)及造血功能，并在機體的抗感染免疫反應(yīng)中起重要作用。
IL-6在多種疾病時有明顯改變，其表達(dá)失調(diào)可引起許多疾病，其臨床表現(xiàn)主要為發(fā)病時IL-6水平增高。在發(fā)生內(nèi)外傷、外科手術(shù)，應(yīng)激反應(yīng)，感染，腦死亡，腫瘤產(chǎn)生以及其它情況的急性炎癥反應(yīng)過程中IL-6會快速生成。手術(shù)病人的IL-6濃度能夠預(yù)示是否會有手術(shù)并發(fā)癥的產(chǎn)生。
連續(xù)檢測重癥監(jiān)護病人血清或血漿中IL-6的水平能有效的評估系統(tǒng)性炎癥反應(yīng)綜合癥（SIRS）的嚴(yán)重程度，膿毒血癥以及膿毒血癥性休克的預(yù)后情況。IL-6的血清濃度水平增高早于PCT和CRP，能作為膿毒血癥的早期警告指標(biāo)。IL-6還在慢性炎癥反應(yīng)（如類風(fēng)濕關(guān)節(jié)炎）中扮演著重要角色。