6-Carboxyfluorescein
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6-Carboxyfluorescein Eigenschaften
- Schmelzpunkt:
- >300 °C(lit.)
- Siedepunkt:
- 736.4±60.0 °C(Predicted)
- Dichte
- 1.73±0.1 g/cm3(Predicted)
- storage temp.
- Sealed in dry,2-8°C
- L?slichkeit
- DMSO: soluble
- Aggregatzustand
- Solid
- pka
- 3.3, 4.6, 6.4, 7.0(at 25℃)
- Farbe
- Yellow to orange
- S?ure-Base-Indikators(pH-Indikatoren)
- Weak green ' uorescence (6.0) to strong green ' uourescence (7.2)
- maximale Wellenl?nge (λmax)
- 492nm
- BRN
- 54341
- Major Application
- Diagnosis of hematologic cancer, nongastric diseases, detection of genetically modified wheat, chromosomes, gene expression, nucleic acid, hepatitis A virus, avian influenza virus subtype H5 and H5N1, SARS virus, herpex simplex virus
- Biologische Anwendungen
- Diagnosing bladder cancer; evaluating corneal endothelial barrier function; as a substrate for measuring protein kinase activity, methyltransferase activity, phospholipase activity, rhodanese activity,sulfotransferase activity;use in ophthalmology
- CAS Datenbank
- 3301-79-9(CAS DataBase Reference)
Sicherheit
- Risiko- und Sicherheitserkl?rung
- Gefahreninformationscode (GHS)
Kennzeichnung gef?hrlicher | Xi | ||
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R-S?tze: | 36/37/38 | ||
S-S?tze: | 26-36/37 | ||
WGK Germany | 3 | ||
F | 10-21 | ||
HS Code | 3204 90 00 |
Bildanzeige (GHS) |
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6-Carboxyfluorescein Chemische Eigenschaften,Einsatz,Produktion Methoden
R-S?tze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.S-S?tze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.S36/37:Bei der Arbeit geeignete Schutzhandschuhe und Schutzkleidung tragen.
Chemische Eigenschaften
Orange SolidVerwenden
6-Carboxyfluorescein (6-FAM) is derivative of fluorescein giving stable derivative upon conjugation with biopolymers.It has been used to check the plasma membrane integrity in sperm cells. The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein.synthetische
1,2,4-Benzenetricarboxylic anhydride (also called 4- carboxyphthalic anhydride, 25.0 g, 0.13 mol) was added to a solution of 1,3-dihydroxybenzene (also called resorcinol, 28.6 g, 0.26 mol) in methane sulfonic acid (1M). An air condenser was attached to the flask and the reaction was heated at 85 oC in an open vessel for 24 h. After cooling to room temperature, the reaction mixture was poured into 7 volumes of ice/water. An orange-yellow precipitate formed; this was collected by filtration and dried in an oven at 200 oC. This residue was recrystallized two times from methanol/hexane to give 1.0 g of 6- carboxyfluorescein methanesulfonic acid adduct preferentially crystallizes from MeOH/hexanes. The mother liquors from this procedure were collected, the solvent was removed in vacuo, and the residues were recrystallized two times from ethanol/hexanes to give 3.2 g of 5-carboxyfluorescein methanesulfonic acid adduct preferentially crystallizes from EtOH/hexanes. Finally, the mother liquors from this experiment were combined, evaporated to dryness, and recrystallized two times from methanol/hexanes to give another 3.0 g of 6-carboxyfluorescein methanesulfonic acid adduct preferentially crystallizes from MeOH/hexanes, making a total yield of 4.0 g, 40 %. Careful dropwise addition of conc. HCl(aq) to solutions of these methanesulfonic esters preferentially crystallizes from EtOH/hexanes and preferentially crystallizes from MeOH/hexanes in 4M sodium hydroxide gave 5-carboxyfluorescein and 6-carboxyfluorescein, respectively in near quantitative yield.Definition
ChEBI: 6-carboxyfluorescein is a monocarboxylic acid. It derives from a fluorescein (lactone form).Application
6-Carboxyfluorescein is a useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.Biochem/physiol Actions
6-Carboxyfluorescein diacetate is a membrane permeable compound. It can be hydrolysed by intracellular esterases to form a membrane impermeable bright green fluorescent molecule, 6-carboxyfluorescein.6-Carboxyfluorescein Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
6-Carboxyfluorescein Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.
Global( 268)Lieferanten
Firmenname | Telefon | Land | Produktkatalog | Edge Rate | |
---|---|---|---|---|---|
Hongxiong | +undefined18915499068 |
Suzhouhongxiong@163.com | China | 120 | 58 |
Shaanxi Dideu Medichem Co. Ltd | +86-029-81138252 +86-18789408387 |
1057@dideu.com | China | 3889 | 58 |
Hebei Chuanghai Biotechnology Co,.LTD | +86-13131129325 |
sales1@chuanghaibio.com | China | 5889 | 58 |
Henan Fengda Chemical Co., Ltd | +86-371-86557731 +86-13613820652 |
info@fdachem.com | China | 20263 | 58 |
Hebei Zhuanglai Chemical Trading Co.,Ltd | +8613343047651 |
admin@zlchemi.com | China | 3692 | 58 |
Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21632 | 55 |
Nanjing ChemLin Chemical Industry Co., Ltd. | 025-83697070 |
product@chemlin.com.cn | CHINA | 3009 | 60 |
career henan chemical co | +86-0371-86658258 +8613203830695 |
sales@coreychem.com | China | 29871 | 58 |
Changzhou Ansciep Chemical Co., Ltd. | +86 519 86305871 |
sales@ansciepchem.com | CHINA | 4241 | 58 |
Accela ChemBio Inc. | +1-858-6993322 |
info@accelachem.com | United States | 18463 | 58 |
3301-79-9()Verwandte Suche:
Fluorescein
3',6'-Dihydroxy-5(oder 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-on
Dinatrium-2-(3-oxo-6-oxidoxanthen-9-yl)benzoat
Luciferase
3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3',6'-diyldiacetat
Carboxy styrene-butadiene latex
5-Carboxyfluorescein
3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-ar-carbonsure
CARBOXYLIC ACID
- 3-Oxo-3',6'-dihydroxy-1,3-dihydrospiro[isobenzofuran-1,9'-[9H]xanthene]-6-carboxylic acid
- 3',6'-dihydroxy-1-keto-spiro[isobenzofuran-3,9'-xanthene]-5-carboxylic acid
- 3',6'-dihydroxy-1-oxo-spiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
- 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
- 6-Carboxyfluorescein,6-FAM, FLUOS
- 6-Carboxyfluorescein Hydrate
- 6-FAM, 6-CF
- 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-6-carboxylic acid
- (3,6,9-Trihydroxyxanthen-9-yl)terephthalic Acid
- 6-FAM, 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-terephthalic acid
- 6-Carboxyfluoroscein
- 6-Carboxyfluoroscein SynonyMs 3',6'-Dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-6-carboxylic acid
- 3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylic acid
- 6-FAM, FLUOS
- 3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid
- 2-(3-hydroxy-6-oxo-6H-xanthen-9-yl)terephthalic acid
- Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylicacid, 3',6'-dihydroxy-3-oxo-
- 6-Carboxyfluorescein, Single isomer
- 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-6-carboxylic acid
- 6-CARBOXYFLUORESCEIN
- 2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-TEREPHTHALIC ACID
- 6-Carboxyfluorescein (6-FAM)
- 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-terepht
- Spiro[phthalan-1,9′-xanthene]-6-carboxylic Acid, 3′,6′-dihydroxy-3-oxo-
- Terephthalicacid, (3,6,9-trihydroxyxanthen-9-yl)-, Γ-lactone
- 6-carboxy' Uorescein
- Fluorescein, 6-carboxy-
- 6-FAM [6-Carboxyfluorescein] *CAS 3301-79-9*
- 6-Carboxyfluorescein Hydrate>
- 6-Carboxyfluorescein,>97%
- 3301-79-9 6-Carboxyfluorescein
- Carboxyfluorescein
- 6-FAM
- 3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylic acid
- 6-FAM (6-Carboxyfluorescein), Amine-reactive fluorescent label
- 6-FAM, 10 mM in DMSO
- 3301-79-9
- C21H12O7H2O
- BioChemical
- Biochemicals and Reagents
- Detection
- Fluorescein and Derivatives
- Fluorescent Labels
- Fluorescent Probes, Labels, Particles and Stains
- marker
- Fluorescent Labels and Indicators
- Fluorescent Labels & Indicators
- Electronic Chemicals
- Fluorescent
- Xanthene
- FLUOROPHORES
- FAM