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5-Carboxyfluorescein

5-Carboxyfluorescein Struktur
76823-03-5
CAS-Nr.
76823-03-5
Englisch Name:
5-Carboxyfluorescein
Synonyma:
5-FAM;5(6)-FAM;5-CF;4-carboxyfluorescein;5-Carboxyfluorescein (5-FAM);5-(AND-6)-CARBOXY SNARF(R)-1;3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid;TQSY001;Fam (dye);5-FAM, 5-CF
CBNumber:
CB1201921
Summenformel:
C21H12O7
Molgewicht:
376.32
MOL-Datei:
76823-03-5.mol

5-Carboxyfluorescein Eigenschaften

Schmelzpunkt:
368-372°C
Siedepunkt:
725.2±60.0 °C(Predicted)
Dichte
1.73±0.1 g/cm3(Predicted)
storage temp. 
Keep in dark place,Sealed in dry,2-8°C
L?slichkeit
DMF: soluble
Aggregatzustand
Solid
pka
3.3, 4.6, 6.4, 7.0(at 25℃)
Farbe
Yellow to orange
S?ure-Base-Indikators(pH-Indikatoren)
Weak green ' uorescence (6.0) to strong green ' uourescence (7.2)
maximale Wellenl?nge (λmax)
492nm
BRN 
57037
Biologische Anwendungen
Authenticating canned products; detecting/measuring citrate; as a substrate for measuring phospholipase activity; use in ophthalmology
Major Application
Fluorescent probe, diagnosis of cancer, leukemia, colorectal cancer, polynucleotide, bacteria, pathogens, nucleic acid, hepatitis A virus, dengue virus, herpes simplex virus, gene, HIV type, cardiovascular risk factor
InChIKey
NJYVEMPWNAYQQN-UHFFFAOYSA-N
CAS Datenbank
76823-03-5(CAS DataBase Reference)
Sicherheit
  • Risiko- und Sicherheitserkl?rung
  • Gefahreninformationscode (GHS)
Kennzeichnung gef?hrlicher Xi
R-S?tze: 36
S-S?tze: 26-39
WGK Germany  3
8
HS Code  32129000
Bildanzeige (GHS) GHS hazard pictograms
Alarmwort Warnung
Gefahrenhinweise
Code Gefahrenhinweise Gefahrenklasse Abteilung Alarmwort Symbol P-Code
H315 Verursacht Hautreizungen. Hautreizung Kategorie 2 Warnung GHS hazard pictogramssrc="/GHS07.jpg" width="20" height="20" /> P264, P280, P302+P352, P321,P332+P313, P362
H319 Verursacht schwere Augenreizung. Schwere Augenreizung Kategorie 2 Warnung GHS hazard pictogramssrc="/GHS07.jpg" width="20" height="20" /> P264, P280, P305+P351+P338,P337+P313P
Sicherheit
P264 Nach Gebrauch gründlich waschen.
P264 Nach Gebrauch gründlich waschen.
P280 Schutzhandschuhe/Schutzkleidung/Augenschutz tragen.
P305+P351+P338 BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach M?glichkeit entfernen. Weiter spülen.
P337+P313 Bei anhaltender Augenreizung: ?rztlichen Rat einholen/?rztliche Hilfe hinzuziehen.

5-Carboxyfluorescein Chemische Eigenschaften,Einsatz,Produktion Methoden

Beschreibung

5-Carboxyfluorescein is a single isomer derivative of 5(6)-carboxyfluorescein that can be used to fluorescently label biomolecules through the interaction of carboxylic acid with primary amines. It demonstrates excitation/emission maxima of 492 and 518 nm, respectively.

Chemische Eigenschaften

Orange Solid

Verwenden

5-Carboxyfluorescein(5-FAM) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. It is a useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. It has been principally used to develop a variety of green fluorescent reagents and small fluorescent molecules due to its relatively high absorbance characteristics, excellent fluorescence quantum yield, and good water solubility. The probe has also found application in studies of liposome-cell, cell-cell, and liposome-liposome interactions and in related studies on lipid bilayer structures.

Definition

ChEBI: 5-carboxyfluorescein is a monocarboxylic acid. It has a role as a fluorochrome. It is functionally related to a fluorescein (lactone form).

Biochem/physiol Actions

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Synthese

5-Carboxyfluorescein is a mixture of 5- and 6-isomers prepared by the reaction of benzotricarboxylic anhydride with resorcinol and zinc chloride.
Reaction: 1,2,4-Benzenetricarboxylic anhydride (also called 4-carboxyphthalic anhydride, 25.0 g, 0.13 mol) was added to a solution of 1,3-dihydroxybenzene (also called resorcinol, 28.6 g, 0.26 mol) in methane sulfonic acid (1M). An air condenser was attached to the flask and the reaction was heated at 85°C in an open vessel for 24 h. After cooling to room temperature, the reaction mixture was poured into 7 volumes of ice/water. An orange-yellow precipitate formed; this was collected by filtration and dried in an oven at 200°C. This residue was recrystallized two times from methanol/hexane to give 1.0 g of 6-carboxyfluorescein methanesulfonic acid adduct 2b. The mother liquors from this procedure were collected, the solvent was removed in vacuo, and the residues were recrystallized two times from ethanol/hexanes to give 3.2 g of 5-carboxyfluorescein methanesulfonic acid adduct 2a.
Preparation of 5- and 6-Carboxyfluorescein
The methane sulfonic esters 2 are easily converted to the 5- and 6-carboxyfluoresceins 1 by treatment with sodium hydroxide solution then neutralizing with aqueous HCl.

5-Carboxyfluorescein Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


5-Carboxyfluorescein Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

Global( 291)Lieferanten
Firmenname Telefon E-Mail Land Produktkatalog Edge Rate
Hongxiong
+undefined18915499068
Suzhouhongxiong@163.com China 120 58
Hebei Kingfiner Technology Development Co.Ltd
+86-15532196582 +86-15373005021
lisa@kingfinertech.com China 3010 58
Henan Fengda Chemical Co., Ltd
+86-371-86557731 +86-13613820652
info@fdachem.com China 20284 58
Henan Tianfu Chemical Co.,Ltd.
+86-0371-55170693 +86-19937530512
info@tianfuchem.com China 21634 55
career henan chemical co
+86-0371-86658258 +8613203830695
sales@coreychem.com China 29880 58
Changzhou Ansciep Chemical Co., Ltd.
+86 519 86305871
sales@ansciepchem.com CHINA 4241 58
Accela ChemBio Inc.
+1-858-6993322
info@accelachem.com United States 17777 58
Chongqing Chemdad Co., Ltd
+86-023-6139-8061 +86-86-13650506873
sales@chemdad.com China 39894 58
SHANGHAI T&W PHARMACEUTICAL CO., LTD.
+86-021-61551413 +8618813727289
contact@trustwe.com China 5738 58
Xi'an Yutbon Pharmaceutical Technology Co., Ltd
029-81140587 +8618717328141
sales@yutbon.com China 454 58

76823-03-5()Verwandte Suche:


  • 5-CARBOXYLUORESCEIN
  • 4-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-isophth
  • 3',6'-dihydroxy-3-keto-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
  • 5-Carboxyfluorescein Hydrate
  • 5-FAM, 5-CF
  • 3',6'-Dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic Acid
  • 5-FAM, 4-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-isophthalic acid
  • 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylicacid, 3',6'-dihydroxy-3-oxo-
  • 3',6'-Dihydroxy-3-oxo-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
  • TQSY001
  • 5-Carboxyfluorescein, Single isomer
  • 5-(AND-6)-CARBOXY SNAFL(R)-1
  • 5-(AND-6)-CARBOXY SNAFL(R)-2
  • 5-(AND-6)-CARBOXYFLUORESCEIN
  • 5-CARBOXYFLUORESCEIN
  • 4-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-ISOPHTHALIC ACID
  • 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
  • 5-carboxy' Uorescein
  • Fam (dye)
  • Fam 494/520
  • 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5*
  • 5-Carboxyfluorescein Hydrate>
  • Carboxyfluorescein Carboxyfluorescein
  • 4-carboxyfluorescein
  • 5-Carboxyfluorescein (5-FAM)
  • 5(6)-FAM
  • 5-(AND-6)-CARBOXY SNARF(R)-1
  • 5-FAM
  • 5-CF
  • 3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
  • 3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
  • 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
  • 76823-03-5
  • C21H12O7xH2O
  • Biochemicals and Reagents
  • BioChemical
  • Fluorescent Probes, Labels, Particles and Stains
  • Detection
  • Fluorescent
  • Xanthene
  • marker
  • Fluorescent Labels & Indicators
  • FLUOROPHORES
  • FAM
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