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4',6-Diamidino-2-phenylindole dihydrochloride

4',6-Diamidino-2-phenylindole dihydrochloride Struktur
28718-90-3
CAS-Nr.
28718-90-3
Englisch Name:
4',6-Diamidino-2-phenylindole dihydrochloride
Synonyma:
DAPI;DAPI.HCl;DAPI 2HCL;DAPI Stain;-Cellstain-Dapi;DAPI(meilunbio);DAPI Stain Solution;4',6-Diamidino-2-phe;DAPI (hydrochloride);DAPI, DIHYDROCHLORIDE
CBNumber:
CB1453377
Summenformel:
C16H16ClN5
Molgewicht:
313.79
MOL-Datei:
28718-90-3.mol

4',6-Diamidino-2-phenylindole dihydrochloride Eigenschaften

Schmelzpunkt:
>300°C (dec.)
storage temp. 
2-8°C
L?slichkeit
H2O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.
Aggregatzustand
powder
Farbe
Clear
PH
4.0-5.0 (10g/l, H2O, 20℃)
maximale Wellenl?nge (λmax)
358 nm, 342 nm
BRN 
4894417
Stabilit?t:
PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months.
Biologische Anwendungen
Nuclear apoptosis assay; targeting drug to cerebral neuron; treating amyloidosis,neurodegenerative diseases,Alzheimer’s disease,Down’s syndrome,type II diabetes; monitoring bioaerosols
InChIKey
FPNZBYLXNYPRLR-UHFFFAOYSA-N
CAS Datenbank
28718-90-3(CAS DataBase Reference)
Sicherheit
  • Risiko- und Sicherheitserkl?rung
  • Gefahreninformationscode (GHS)
Kennzeichnung gef?hrlicher Xi,Xn
R-S?tze: 36/37/38-20/21/22
S-S?tze: 26-36-24/25-22-37/39
WGK Germany  3
RTECS-Nr. NL5995050
3-8-10-21
HS Code  29339900
Bildanzeige (GHS) GHS hazard pictograms
Alarmwort Warnung
Gefahrenhinweise
Code Gefahrenhinweise Gefahrenklasse Abteilung Alarmwort Symbol P-Code
H315 Verursacht Hautreizungen. Hautreizung Kategorie 2 Warnung GHS hazard pictogramssrc="/GHS07.jpg" width="20" height="20" /> P264, P280, P302+P352, P321,P332+P313, P362
H317 Kann allergische Hautreaktionen verursachen. Sensibilisierung der Haut Kategorie 1A Warnung GHS hazard pictogramssrc="/GHS07.jpg" width="20" height="20" /> P261, P272, P280, P302+P352,P333+P313, P321, P363, P501
H335 Kann die Atemwege reizen. Spezifische Zielorgan-Toxizit?t (einmalige Exposition) Kategorie 3 (Atemwegsreizung) Warnung GHS hazard pictogramssrc="/GHS07.jpg" width="20" height="20" />
Sicherheit
P261 Einatmen von Staub vermeiden.
P264 Nach Gebrauch gründlich waschen.
P264 Nach Gebrauch gründlich waschen.
P271 Nur im Freien oder in gut belüfteten R?umen verwenden.
P272 Kontaminierte Arbeitskleidung nicht au?erhalb des Arbeitsplatzes tragen.
P280 Schutzhandschuhe/Schutzkleidung/Augenschutz tragen.
P302+P352 BEI BERüHRUNG MIT DER HAUT: Mit viel Wasser/... (Hersteller kann, falls zweckm??ig, ein Reinigungsmittel angeben oder, wenn Wasser eindeutig ungeeignet ist, ein alternatives Mittel empfehlen) waschen.

4',6-Diamidino-2-phenylindole dihydrochloride Chemische Eigenschaften,Einsatz,Produktion Methoden

R-S?tze Betriebsanweisung:

R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R20/21/22:Gesundheitssch?dlich beim Einatmen,Verschlucken und Berührung mit der Haut.

S-S?tze Betriebsanweisung:

S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
S22:Staub nicht einatmen.
S37/39:Bei der Arbeit geeignete Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.

Beschreibung

DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.

Chemische Eigenschaften

Yellow Solid

Verwenden

4',6-Diamidino-2-phenylindole dihydrochloride is used as a cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine.It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.

Allgemeine Beschreibung

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

Einzelnachweise

[1] W. C. RUSSELL D. H W CAROL NEWMAN. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses[J]. Nature, 1975, 253 5491: 461-462. DOI:10.1038/253461a0.
[2] BETTY I. TARNOWSKI James H N F Spinale. DAPI as a useful stain for nuclear quantitation.[J]. Biotechnic & Histochemistry, 1991, 22 1: 297-302. DOI:10.3109/10520299109109990.
[3] ANA KATRINA ESTANDARTE. The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes.[J]. Scientific Reports, 2016: 31417. DOI:10.1038/srep31417.

4',6-Diamidino-2-phenylindole dihydrochloride Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


4',6-Diamidino-2-phenylindole dihydrochloride Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

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28718-90-3()Verwandte Suche:


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  • 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) for microscopy
  • Indole-6-carboxamidine, 2-(p-amidinophenyl)-, dihydrochloride
  • 40,6-diamidino-2-phenylindole dihydrochloride
  • 6-amidino-2-(4-amidinophenyl) indole Dihydrochloride
  • 4'',6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE CRYSTALLINE
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