92953-10-1
中文名稱(chēng)
氯非銨甲苯磺酸鹽
英文名稱(chēng)
CLOFILIUM TOSYLATE
CAS
92953-10-1
分子式
C28H44ClNO3S
分子量
510.17
MOL 文件
92953-10-1.mol
更新日期
2024/06/03 14:41:41
92953-10-1 結(jié)構(gòu)式
基本信息
中文別名
氯非銨甲苯磺酸鹽 英文別名
CLOFILIUM TOSYLATE4-CHLORO-N,N-DIETHYL-N-HEPTYLBENZENEBUTANAMINIUM TOSYLATE
4-chloro-n,n-diethyl-n-heptyl-benzenebutanaminiusaltwith4-methylbenzenes
N-(4-(4-CHLOROPHENYL)BUTYL)-N,N-DIETHYLHEPTAN-1-AMINIUM 4-METHYLBENZENESULFONATE
所屬類(lèi)別
化學(xué)試劑：有機(jī)試劑
常見(jiàn)問(wèn)題列表
生物活性
Clofilium tosylate 是一種鉀通道 (potassium channel) 阻滯劑，作用于人早幼粒細(xì)胞白血病 (HL-60) 細(xì)胞，激活 caspase-3 而誘導(dǎo)凋亡。具有抗心律失常作用。靶點(diǎn)
Potassium channel
體外研究
HL-60 cells treated with Clofilium (0-20 μM; 24, 48, and 72 hours) lead to suppression of viability and proliferation in both time and concentration-dependent manners. Cell viability decreases significantly in HL-60 cells treated with 2.5 μM to 10 μM of Clofilium.
Clofilium (10 μM, 12 hours) induces the proteolytic cleavage of inactive procaspase-3, p34 into its active form, p17 and subsequent cleavage of its substrate PARP at 2 h after exposure to 10 mM Clofilium. However, there is no significant change in expression of Bcl-2 and Bax proteins.
Cell Viability Assay
| Cell Line: | HL-60 cells |
| Concentration: | 0-20 μM |
| Incubation Time: | 24, 48, and 72 hours |
| Result: | Inhibited HL-60 cells with IC 50 s of 6.3 μM for 24 hours, 3.4 μM for 48 hours, 2.4 μM for 72 hours, respectively. |
Western Blot Analysis
| Cell Line: | HL-60 cells |
| Concentration: | 10 μM |
| Incubation Time: | 12 hours |
| Result: | Induced proteolytic cleavage of caspase-3 and subsequent cleavage of its substrate, PARP, while Bcl-2 and Bax proteins were unaltered. |