429653-73-6
中文名稱
429653-73-6
英文名稱
Y16
CAS
429653-73-6
分子式
C24H20N2O3
分子量
384.43
MOL 文件
429653-73-6.mol
更新日期
2024/10/25 11:12:37
429653-73-6 結(jié)構(gòu)式
基本信息
中文別名
化合物Y16G蛋白偶聯(lián)RHO GEFS抑制劑(Y16)
英文別名
Y16Y16, >98%
Y16, 429653-73-6
4-[[3-[(3-Methylphenyl)methoxy]phenyl]methylene]-1-phenyl-3,5-pyrazolidinedione
3,5-Pyrazolidinedione, 4-[[3-[(3-methylphenyl)methoxy]phenyl]methylene]-1-phenyl-
所屬類別
生物化工：激動劑抑制劑物理化學(xué)性質(zhì)
密度1.273±0.06 g/cm3(Predicted)
儲存條件2-8°C
溶解度DMSO：可溶5mg/mL，澄清（加熱）
酸度系數(shù)(pKa)8.84±0.20(Predicted)
形態(tài)粉末
顏色淡黃色至深橙色
常見問題列表
生物活性
Y16是G蛋白偶聯(lián)Rho GEFs抑制劑。它能阻止LARG和Rho的結(jié)合，Kd值為80 nM。靶點
| Target | Value |
|
RhoGEFs
() |
體外研究
Y16 (10-30 μΜ; 24 hours; NIH 3T3 cells) could inhibit RhoA-GTP formation induced by serum dose dependently and is specific for RhoA.
Y16 (10-30 μΜ; 24 hours; NIH 3T3 cells) efficiently inhibits serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which are downstream of RhoA.
Cell Viability Assay
| Cell Line: | NIH 3T3 cells |
| Concentration: | 10 μΜ, 30 μΜ |
| Incubation Time: | 24 hours |
| Result: | Inhibited RhoA-GTP formation induced by serum dose dependently and was specific for RhoA. |
Western Blot Analysis
| Cell Line: | NIH 3T3 cells |
| Concentration: | 10 μΜ, 30 μΜ |
| Incubation Time: | 24 hours |
| Result: | Inhibited serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which were downstream of RhoA. |