1784-03-8
1784-03-8 結(jié)構(gòu)式
基本信息
4,5-羥基萘乙烯
NΑ-P-甲苯磺?；?L-精氨酸甲酯鹽酸鹽
N-Α-甲苯磺酰-L-精氨酸甲酯鹽酸鹽
N-Α-甲苯磺酰精氨酸甲酯鹽酸鹽
N-對甲苯磺?；?L-精氨酸甲酯鹽酸鹽
L-TAME
N-ALPHA-4-TOSYL-L-ARGININE METHYL ESTER HYDROCHLORIDE
NALPHA-P-TOLUENESULFONYL-L-ARGININE METHYL ESTER HYDROCHLORIDE
NALPHA-P-TOSYL-L-ARGININE METHYL ESTER HCL
N-ALPHA-P-TOSYL-L-ARGININE METHYL ESTER HYDROCHLORIDE
N-ALPHA-P-TOSYL-L-ARGININE METHYL ESTER HYDROCHLORIDE SALT
N-ALPHA-TOLUENESULFONYLARGININE METHYL ESTER HYDROCHLORIDE
NALPHA-TOSYL-L-ARGININE METHYL ESTER HYDROCHLORIDE
N-ALPHA-TOSYL-L-ARGININE METHYL ESTER HYDROCHLORIDE SALT
N-A-P-TOSYL-L-ARGININE METHYL ESTER HYDROCHLORIDE
N-P-TOSYL-L-ARGININE METHYL ESTER HYDROCHLORIDE
P-TOLUENESULFONYL-L-ARGININE METHYL ESTER HCL
P-TOLUENESULFONYL-L-ARGININE METHYL ESTER HYDROCHLORIDE
P-TOSYL-L-ARG METHYL ESTER HCL
TAME
TAME HYDROCHLORIDE
TOS-ARGININE-OME HCL
TOS-ARG-OME HCL
TOSYL-L-ARGININE METHYL ESTER HYDROCHLORIDE
物理化學(xué)性質(zhì)
常見問題列表
圖1 NA-P-甲苯磺酰-L-精氨酸甲酯鹽酸鹽合成反應(yīng)式
將L-精氨酸26.25g(0.25mol)、4mol/L氫氧化鈉溶液62.5mL(0.25mol)加入裝有機械攪拌、溫度計、酸度計、滴加瓶的500mL四口燒瓶中，在冷阱中開啟攪拌，使精氨酸和氫氧化鈉反應(yīng)完全，向四口燒瓶中加入2.7g四丁基硫酸氫銨和50mL水及甲醇攪拌使相轉(zhuǎn)移催化劑溶解，0℃以下緩慢滴加對甲基苯磺酰氯42.5mL(0.3mol)，后期不斷滴加pH=9的硼酸緩沖溶液調(diào)節(jié)pH保持在9左右。對甲基苯磺酰氯滴完后將溫度升至15℃，繼續(xù)攪拌反應(yīng)3h，TLC檢測反應(yīng)完全。用乙醚洗滌(2×20mL)，醚層棄去。不斷攪拌下緩慢滴加6mol/L鹽酸，調(diào)節(jié)水相pH=2，繼續(xù)攪拌20min，析出白色固體。乙酸乙酯萃取(3×30mL)，分出乙酸乙酯有機層。用質(zhì)量分數(shù)為5%氯化鈉溶液和水洗至中性，無水硫酸鈉干燥，旋蒸除乙酸乙酯，得油狀液體。置于冰箱冷凍過夜，油狀液體析出大量白色固體，加入大量石油醚，攪拌得白色粉末狀固體。抽濾，用石油醚洗滌、干燥，得NA-P-甲苯磺酰-L-精氨酸甲酯鹽酸鹽。
Anaphase-promoting complex (APC)
The absence of APC substrates, TAME hydrochloride ejects Cdc20 from the APC by promoting Cdc20 auto-ubiquitination in its N-terminal region. Cyclin B1 antagonizes TAME hydrochloride's effect by promoting binding of free Cdc20 to the APC and suppressing Cdc20 auto-ubiquitination.
TAME hydrochloride stabilizes cyclin B1 in
Xenopus
extract by two mechanisms. First, it reduces the k
cat
of the APC
Cdc20
/cyclin B1 complex without affecting the K
m
, slowing the initial ubiquitination of unmodified cyclin B1. Second, as cyclin B1 becomes ubiquitinated, it loses its ability to promote Cdc20 binding to the APC in the presence of TAME hydrochloride. As a result, cyclin B1 ubiquitination terminates before reaching the threshold necessary for proteolysis.
知名試劑公司產(chǎn)品信息
Nalpha-4-Tosyl-L-arginine methyl ester hydrochloride, 99%(1784-03-8)
| 報價日期 | 產(chǎn)品編號 | 產(chǎn)品名稱 | CAS號 | 包裝 | 價格 |
| 2024/11/08 | 13920 | NA-P-甲苯磺酰-L-精氨酸甲酯鹽酸鹽 Nalpha-4-Tosyl-L-arginine methyl ester hydrochloride, 98+% | 1784-03-8 | 5g | 610元 |
| 2024/11/08 | 13920 | NA-P-甲苯磺酰-L-精氨酸甲酯鹽酸鹽 Nalpha-4-Tosyl-L-arginine methyl ester hydrochloride, 98+% | 1784-03-8 | 10g | 976元 |
| 2024/11/08 | 13920 | NA-P-甲苯磺酰-L-精氨酸甲酯鹽酸鹽 Nalpha-4-Tosyl-L-arginine methyl ester hydrochloride, 98+% | 1784-03-8 | 50g | 3924元 |