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ChemicalBook CAS DataBase List 5-(METHOXYMETHYL)-2-FUROIC ACID
1917-60-8

5-(METHOXYMETHYL)-2-FUROIC ACID synthesis

4synthesis methods
-

Yield:1917-60-8 95 %Chromat.

Reaction Conditions:

with recombinant Escherichia coli cells expressing 3-succinoylsemialdehyde-pyridine dehydrogenase from Comamonastestosteroni SC1588 in aq. phosphate buffer at 30; pH=7; for 5 h;Enzymatic reaction;

Steps:

2.3. General procedure for biocatalytic oxidation of FF

General procedure: Typically, 4 mL of phosphate buffer (0.2 M, pH 7) containing 50mMFF and 50 mg (cell wet weight) per mL microbial cells was incubated at30 °C and 160 r/min. Aliquots were withdrawn from the reaction mixturesat specified time intervals and diluted with the correspondingmobile phase prior to HPLC analysis. The conversion was defined as theratio of the consumed substrate amount to the initial substrate amount(in mol). The yield was defined as the ratio of the formed productamount to the theoretical value based on the initial substrate amount(in mol). The selectivity was defined as the ratio of the formed productamount to the total amount of all products (in mol). All the experimentswere conducted at least in duplicate, and the values were expressed asthe means ± standard deviations.

References:

Shi, Sai-Sai;Zhang, Xue-Ying;Zong, Min-Hua;Wang, Chuan-Fu;Li, Ning [Molecular catalysis,2019,vol. 469,p. 68 - 74]

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