ChemicalBook CAS DataBase List 3-METHYL-2-OXOBUTYRIC ACIDDISCONTINUED

3-METHYL-2-OXOBUTYRIC ACIDDISCONTINUED synthesis

8synthesis methods

Product Name:3-METHYL-2-OXOBUTYRIC ACIDDISCONTINUED
CAS Number:759-05-7
Molecular formula:C5H8O3
Molecular Weight:116.12

328-50-7 Synthesis

328-50-7
563 suppliers
$6.00/25g

640-68-6
444 suppliers
$8.00/10g

6893-26-1
464 suppliers
$6.00/10g

759-05-7
108 suppliers
$50.00/50mg

Yield:-

Reaction Conditions:

with pyridoxal 5'-phosphate;recombinant Lactobacillus salivarius UCC₁₁₈ D-amino acid aminotransferase in aq. phosphate buffer; pH=7.5 at 30; for 0.0166667 h;Enzymatic reaction;

Steps:

General procedure: Amino donor specificity was determined using UPLC by measuring the rate of D-glutamate formation from α-ketoglutarate in the presence of various D-amino acids. The standard reaction mixture (1 ml) contained 100 mM potassium phosphate buffer (pH 7.5), 50 mM D-amino acid, 20 mM α-ketoglutarate, 0.05 mM pyridoxal-5′-phosphate (PLP) and the purified D-AAT. The enzyme reaction was run at 30 °C for 1 min and was stopped by adding 1 ml of 20% trichloroacetic acid. The reaction mixture was then incubated for 1 min on ice and 0.5 ml of 4 M NaOH was added to neutralize the mixture. UPLC analysis was performed using an ACQUITY UPLC TUV system consisting of a Waters Binary Solvent Manager, Sample Manager, FLR Detector and AccQ-Tag Ultra 2.1 × 100-mm column (Waters, Tokyo, Japan) with an eluent flow rate of 0.25 ml/min [14]. The column temperature was 30 °C, and the fluorescent wavelengths used for the FLR Detector were 350 and 450 nm. The eluent was linearly graduated using 85% 50 mM sodium acetate buffer (pH 5.9) and 15% acetonitrile.

References:

Kobayashi, Jyumpei;Shimizu, Yasuhiro;Mutaguchi, Yuta;Doi, Katsumi;Ohshima, Toshihisa [Journal of Molecular Catalysis B: Enzymatic,2013,vol. 94,p. 15 - 22]

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