成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

ChemicalBook >> journal list >> Bioresource Technology >>article
Bioresource Technology

Bioresource Technology

IF: 9.69
Download PDF

Production of capsaicinoid nonivamide from plant oil and vanillylamine via whole-cell biotransformation

Published:21 October 2023 DOI: 10.1016/j.biortech.2023.129883 PMID: 37871741
Jianzhong Ge , Jie Zhang , Xiaolu Wang , Yingguo Bai , Yaru Wang , Yuan Wang , Tao Tu , Xing Qin , Xiaoyun Su , Huiying Luo , Bin Yao , Huoqing Huang

Abstract

Capsaicinoids are mostly derived from chili peppers and have widespread applications in food, feed, and pharmacology. Compared with plant extraction, the use of microbial cell factories for capsaicinoids production is considered as a more efficient approach. Here, the biotransformation of renewable plant oil and vanillylamine into capsaicinoid nonivamide was investigated. Nonivamide biosynthesis using nonanoic acid and vanillylamine as substrates was achieved in Escherichia coli by heterologous expression of genes encoding amide-forming N-acyltransferase and CoA-ligase. Through increasing nonanoic acid tolerance of chassis cell, screening key enzymes involved in nonivamide biosynthesis and optimizing biotransformation conditions, the nonivamide titer reached 0.5?g/L. By further integrating a route for conversion of oleic acid to nonanoic acid, nonivamide biosynthesis was finally achieved using olive oil and vanillylamine as substrates, yielding a titer of approximately 10.7?mg/L. Results from this study provide valuable information for constructing highly efficient cell factories for the production of capsaicinoid compounds.

Substances (5)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Oleic acid 112-80-1 C18H34O2 1070 suppliers $5.00-$2148.30
Nonivamide 2444-46-4 C17H27NO3 588 suppliers $5.00-$3407.25
Nonanoic acid 112-05-0 C9H18O2 587 suppliers $8.00-$1900.00
Olive oil 8001-25-0 N/A 301 suppliers $50.00-$462.00
Vanillylamine Hydrochloride, 1 g - Inquiry

Similar articles

IF:0

Erythropoietin regulation of red blood cell production: from bench to bedside and back.

F1000Research Senthil Velan Bhoopalan,?Lily Jun-Shen Huang,etc Published: 18 September 2020
IF:2.5

Production of chlorphenesin galactoside by whole cells of β-galactosidase-containing Escherichia coli.

Journal of microbiology and biotechnology Sang-Eun Lee, Hyang-Yeol Lee,etc Published: 28 June 2013
IF:0

Castor Oil: Properties, Uses, and Optimization of Processing Parameters in Commercial Production

Lipid insights V. Patel,?Gerard G. Dumancas,etc Published: 7 September 2016