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Food and Chemical Toxicology

Food and Chemical Toxicology

IF: 3.89
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Expression, characterization, and application of an aldo-keto reductase mined from Bacillus velezensis Vel-HNGD-F2 for deoxynivalenol biodegradation

Published:27 November 2024 DOI: 10.1016/j.fct.2024.115159 PMID: 39613245
Junxia Liu, Peng Li, Hang Jia

Abstract

Deoxynivalenol (DON) contamination in cereals and their products poses a potential threat to animal and human health, however, physical and chemical detoxification methods deplete nutrients and cannot specifically remove DON. This study aims to identify novel and efficient DON-degrading enzymes, providing practical support for their application in biodegradation. A novel DON-degrading aldo-keto reductase named AKR11A2 was identified from Bacillus velezensis Vel-HNGD-F2 through BlastP comparison. AKR11A2, an enzyme with a molecular mass of 34.8 kDa encoded by a 933 bp gene, exhibited optimal activity at pH 9 and 40°C, while demonstrating remarkable thermal and alkaline stability by retaining over 90% of its activity. UPLC-MS/MS analysis revealed that the m/z of the DON degradation product was 295.1, identified as 3-epi-DON, formed through the direct isomerization of DON. Notably, zebrafish experiments demonstrated that the liver toxicity of the degradation product was significantly lower than that of DON. AKR11A2 effectively degraded 50.69% of the DON in contaminated corn, highlighting its practical application in food safety. These findings indicate that the study achieved the biodegradation of DON and provide a promising theoretical and technological support for the application of DON detoxifying enzymes in food and feed products.

Substances (1)

Materials
Procduct Name CAS Molecular Formula Supplier Price
KANAMYCIN 59-01-8 C18H36N4O11 193 suppliers $25.00-$1372.96

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