成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

ChemicalBook >> journal list >> Ministry of Science and Technology, Vietnam >>article

Ministry of Science and Technology, Vietnam

Download PDF

Development of expression vector with the Pgrac100 promoter induced by IPTG and integrated gfp+ gene into Bacillus subtilisgenome at lacA locus

Published:25 January 2024 DOI: 10.31276/vjst.66(1).75-80
Thi Bich Phuong Chu, D. Nguyen, Thi Phuong Trang Phan

Abstract

Bacillus subtilis is a prospective gram - positive bacterium that offers an opportunity for the production of recombinant proteins in the pharmaceutical, agricultural, industrial, and food industries.The goal of this study was to develop an integrative inducible expression system by incorporating the Pgrac100 promoter into the lacA locus of the B. subtilis genome. The results showed that the Pgrac100 promoter was able to strictly control the expression of the target protein, with induction factors of 3.3, 7.6, and 10.8 after 4 hours under induction with 0.01, 0.1, and 1 mM Isopropulβ-D-1-thiogalactopyranoside (IPTG), respectively. The duration of induction and the concentration of IPTG had significant effects on the efficacy of target protein expression. Specifically, when induced with 0.1 mM IPTG after 4 hours, there was a significant rise in GFP expression, accounting for 13% of the total cellular protein. Our new vector system provided more options for the production of recombinant proteins in basic research and industrial applications.

Substances (2)

Related products
Procduct Name CAS Molecular Formula Supplier Price
IPTG 367-93-1 C9H18O5S 810 suppliers $5.00-$30622.00
RGFP PROTEIN 55 suppliers Inquiry

Similar articles

IF:0

Expression of Cloned Genes in E. coli Using IPTG-inducible Promoters.

CSH protocols Joseph Sambrook, David W Russell,etc Published: 1 June 2006
IF:0

Exploring the effect of capsaicin on gene expression and chemotherapy sensitization in gastric cancer cells

bioRxiv - Cancer Biology Weijian Meng, Sophia Xie,etc Published: 7 September 2024
IF:5.2

L-Histidine attenuates NEFA-induced inflammatory responses by suppressing Gab2 expression

Life sciences Mengze Li , Huiying Wang ,etc Published: 4 May 2024