稱 Tris-Base（三羥甲基氨基甲烷）

規(guī)格 100g 

保存 常溫保存，長期有效

產(chǎn)品簡介 

Tris base，即Tris堿，中文名也稱為三(羥甲基)氨基甲烷，即生物化學和分子生物學中常常被配制成多種緩沖液，例如：Tris-EDTA（TE緩沖液），多用于核酸的純化和儲存，保護其不被核酸酶降解；Tris acetate-EDTA（TAE緩沖液）和Tris borate-EDTA（TBE緩沖液）普遍用作凝膠配制緩沖液以及電泳緩沖液。

Tris緩沖液不僅被廣泛用作核酸和蛋白質(zhì)的溶劑，還有許多重要用途。Tris被用于不同pH條件下的蛋白質(zhì)晶體生長。Tris緩沖液的低離子強度特點可用于線蟲（C. elegans）核纖層蛋白（lamin）的中間纖維的形成。Tris也是蛋白質(zhì)電泳緩沖液的主要成分之一。此外，Tris還是制備表面活性劑、硫化促進劑和一些藥物的中間物。Tris也被用作滴定標準物。

緩沖特性

Tris為弱堿，在室溫（25℃）下，它的pKa為8.1；根據(jù)緩沖理論，Tris緩沖液的有效緩沖范圍在pH7.0到9.2之間。

Tris堿的水溶液pH在10.5左右，一般加入鹽酸以調(diào)節(jié)pH值至所需值，即可獲得該pH值的緩沖液。但同時應注意溫度對于Tris的pKa的影響。

產(chǎn)品性質(zhì) 

中文別名（Chinese synonym） 2-氨基-2-(羥甲基)-1,3-丙二醇；Tris 堿；氨基丁三醇；三(羥甲基)氨基甲烷；
英文別名（English synonym） THAM; Trizma base; Trometamol; Tris(hydroxymethyl)aminomethane;
2-Amino-2-(hydroxymethyl)-1,3-propanediol;
CAS號（CAS NO.） 77-86-1
分子式（Formula） NH2C(CH2OH)3
分子量（Molecular weight） 121.14
外觀（Appearance） 白色結晶粉末至針狀結晶
熔點（Melting point） 168-172 °C
溶解性（Solubility） 溶于水

注意事項 

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Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids.

Buffering features
The conjugate acid of Tris has a pKa of 8.07 at 25 °C, which implies that the buffer has an effective pH range between 7.1 and 9.1 (pKa ± 1) at room temperature.

Buffer details
The pKa declines approximately 0.03 units per degree Celsius rise in temperature.
Silver-containing single-junction pH electrodes (e.g., silver chloride electrodes) are incompatible with tris since an Ag-tris precipitate forms which clogs the junction. Double-junction electrodes are resistant to this problem, and non-silver containing electrodes are immune.

Other names
TRIS, Tris, Tris base, Tris buffer, Trizma, Trisamine, THAM, Tromethamine, Trometamol, Tromethane, Trisaminol