TBS 緩沖液是生物學(xué)中常使用的等滲緩沖鹽溶液,主要用于免疫組化和原位雜交,酶聯(lián)免疫等實(shí)驗(yàn)中,清 洗免疫印跡實(shí)驗(yàn)中非特異性結(jié)合的抗體等。由 Tris-HCl 構(gòu)成穩(wěn)定的 PH 緩沖體系,NaCl 提供等滲條件。
4℃保存可延長產(chǎn)品保質(zhì)期,長期不用建議低溫保存。若產(chǎn)品出現(xiàn)結(jié)晶析出,請置于 37℃水浴至完全溶解。
常溫運(yùn)輸與存儲。
Tris-buffered saline (abbreviated TBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Tris (with HCl) has a slightly alkaline buffering capacity in the 7–9.2 range.
TBS has many uses because it is isotonic and non-toxic. It can be used to dilute substances used in laboratory experiments. Additives can be used to add to a compound's functionality.
TBS is often used in immuno-blotting for both membrane washing and antibody dilution.
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