| 產(chǎn)品編號 |
產(chǎn)品名稱 |
產(chǎn)品包裝 |
產(chǎn)品價格 |
| AF1123 |
Caspase-2 Rabbit Monoclonal Antibody |
50μl |
618.00元 |
|
來源
|
用途
|
交叉反應(yīng)性
|
分子量
|
|
Rabbit
|
WB, IP, IF, IHC, ICC, FC
|
H, M, R
|
48/30/12 kDa
|
???????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
???????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
???????配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
???????建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1000-1:2000
|
1:50-1:200
|
1:50-1:200
|
1:50-1:200
|
1:50-1:200
|
1:50-1:100
|
-
|
-
|
抗體詳細(xì)信息如下:
|
About this Antibody
|
|
Name
|
Caspase-2 Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
Affinity purification
|
|
About the Immunogen
|
|
Immunogen
|
Recombinant protein
|
|
Gene ID
|
835(Human); 12366(Mouse); 64314(Rat)
|
|
SwissProt
|
P42575(Human); P29594(Mouse); P55215(Rat)
|
|
Synonyms
|
ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
|
|
Category
|
Apoptosis
|
|
Background
|
Caspase-2 (Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated in response to various apoptotic stimuli. Caspase-2 is unique among the caspases in that it has features of both upstream caspases (long prodomain) and downstream caspases (DEXD substrate specificity). Caspase-2 is highly expressed in the brain during development, and is expressed at low levels in adult tissue. Specifically, caspase-2 localizes to the mitochondria, the Golgi, the cytoplasm, and the nucleus. Caspase-2 exists as two isoforms, caspase-2L and caspase-2S, which are produced by alternative splicing and differ in their N and C-termini. Caspase-2L acts as a positive regulator of apoptosis, whereas caspase-2S functions as a negative regulator of apoptosis. Following apoptotic stimuli, the caspase-2L precursor undergoes cleavage at Asp-153 to produce a fragment (p30). The p30 fragment undergoes further cleavage to generate a fragment containing amino acids 153-308 (p18) and a fragment containing amino acids 317-435 (p13 or p14). As apoptosis progresses, the p13 (p14) fragment can undergo further processing to yield a fragment containing amino acids 331-435 (p12).
|
包裝清單:
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
AF1123
|
Caspase-2 Rabbit Monoclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說明書
|
1份
|
保存條件:
???????Caspase-2 Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
???????如果本抗體用于Western?blot?(WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
???????回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
???????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
???????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
???????為了您的安全和健康，請穿實驗服并戴一次性手套操作。