| 產(chǎn)品編號 |
產(chǎn)品名稱 |
產(chǎn)品包裝 |
產(chǎn)品價格 |
| AF0138 |
E-Cadherin Mouse Monoclonal Antibody |
50μl |
416.00元 |
|
來源
|
用途
|
交叉反應(yīng)性
|
分子量
|
|
Mouse
|
WB, ICC, IHC, FC, IF
|
H, M
|
130KDa
|
??????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
??????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
??????配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
??????建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:500-1:1000
|
-
|
1:200
|
1:200
|
1:200
|
1:50-1:100
|
-
|
-
|
抗體詳細(xì)信息如下:
|
About this Antibody
|
|
Name
|
E-Cadherin Mouse Monoclonal Antibody
|
|
Category
|
Monoclonal antibody(mAb); Primary antibody
|
|
Isotype
|
IgG1
|
|
Purification
|
ProA affinity purified
|
|
About the Immunogen
|
|
Immunogen
|
This antibody is produced by immunizing mice with recombinant protein to a region of E-cadherin.
|
|
Gene ID
|
999
|
|
SwissProt
|
P12830
|
|
Synonyms
|
CADH1; cadherin 1; type 1; E-cadherin (epithelial) (ECAD); cadherin-1; CAM 120/80; E-cadherin; epithelial-cadherin; uvomorulin
|
|
Category
|
Adhesion/ECM
|
|
Background
|
E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer.
|
包裝清單：
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
AF0138
|
E-Cadherin Mouse Monoclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀釋液
|
50ml
|
|
—
|
說明書
|
1份
|
保存條件：
??????E-Cadherin Mouse Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
??????如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
??????回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
??????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
??????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
??????為了您的安全和健康，請穿實驗服并戴一次性手套操作。