| 產(chǎn)品編號 |
產(chǎn)品名稱 |
產(chǎn)品包裝 |
產(chǎn)品價格 |
| AF0147 |
ERK2 Rabbit Polyclonal Antibody |
50μl |
416.00元 |
| 來源 |
用途 |
交叉反應(yīng)性 |
分子量 |
| Rabbit |
WB, FC, IHC, ICC, IF |
H, M, R |
42KDa |
??????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
??????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
??????配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
??????建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
| WB |
IP |
IF |
IHC |
ICC |
FC |
ELISA |
ChIP |
| 1:1000-1:2000 |
- |
1:200 |
1:200 |
1:200 |
1:100-1:200 |
- |
- |
抗體詳細信息如下:
| About this Antibody |
| Name |
ERK2 Rabbit Polyclonal Antibody |
| Category |
Polyclonal antibody(pAb); Primary antibody |
| Isotype |
IgG |
| Purification |
Peptide affinity purified |
| About the Immunogen |
| Immunogen |
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to c-terminal sequence of ERK2. |
| Gene ID |
5594 |
| SwissProt |
P28482 |
| Synonyms |
ERK; p38; p40; p41; ERT1; MAPK2; PRKM1; P42MAPK; p41mapk; MAPK1 |
| Category |
MAP Kinase Signaling |
| Background |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. |
包裝清單：
| 產(chǎn)品編號 |
產(chǎn)品名稱 |
包裝 |
| AF0147 |
ERK2 Rabbit Polyclonal Antibody |
50μl |
| AZ050 |
Western一抗稀釋液 |
50ml |
| — |
說明書 |
1份 |
保存條件：
??????ERK2 Rabbit Polyclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
??????如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
??????回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
??????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
??????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
??????為了您的安全和健康，請穿實驗服并戴一次性手套操作。