價格 | 詢價 |
包裝 | 1mg |
最小起訂量 | 1mg |
發(fā)貨地 | 上海 |
更新日期 | 2024-12-25 |
中文名稱:Recombinant Human Vimentin | 英文名稱:Recombinant Human Vimentin |
有效期: 一年 | 產(chǎn)品類別: 重組蛋白 其他重組蛋白 |
產(chǎn)品編號 | 產(chǎn)品名稱 | 產(chǎn)品包裝 | 產(chǎn)品價格 |
P7262-1mg | Recombinant Human Vimentin | 1mg | 5665.00元 |
Species | Gene ID | Accession | Source | Length | MW | Tag |
Human | 7431 | P08670 | E. coli | 465aa | 53.5kDa | - |
About this protein | |
Name | Recombinant Human Vimentin (Recombinant Human Vimentin; rHuVimentin);重組人波形蛋白 |
Synonyms | VIM; FLJ36605; vimentin |
Purity | >95% by SDS-PAGE and HPLC analyses. |
Biological Activity | Data not available. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2μm filtered solution in 30% Acetonitrile and 0.1% TFA. |
Endotoxin | Less than 0.1EU/μg of rHuVimentin as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category | Others |
Background | Vimentin is a type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells, so it is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. Vimentin is a homopolymer assembled from elementary dimers which is mediated by the central alpha-helical coiled-coil rod region. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol and it is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Also, vimentin is found to control the transport of low-density lipoprotein, LDL, -derived cholesterol from alysosome to the site of esterification. Recombinant human vimentin contains 465 amino acid residues and it shares 97%a.a. sequence identity with murine and rat vimentin. |
Amino Acid Sequence | STRSVSSSSY RRMFGGPGTA SRPSSSRSYV TTSTRTYSLG SALRPSTSRS LYASSPGGVY ATRSSAVRLR SSVPGVRLLQ DSVDFSLADA INTEFKNTRT NEKVELQELN DRFANYIDKV RFLEQQNKIL LAELEQLKGQ GKSRLGDLYE EEMRELRRQV DQLTNDKARV EVERDNLAED IMRLREKLQE EMLQREEAEN TLQSFRQDVD NASLARLDLE RKVESLQEEI AFLKKLHEEE IQELQAQIQE QHVQIDVDVS KPDLTAALRD VRQQYESVAA KNLQEAEEWY KSKFADLSEA ANRNNDALRQ AKQESTEYRR QVQSLTCEVD ALKGTNESLE RQMREMEENF AVEAANYQDT IGRLQDEIQN MKEEMARHLR EYQDLLNVKM ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVINETSQH HDDLE |
包裝清單:
產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝 |
P7262-20μg | Recombinant Human Vimentin | 20μg |
P7262-100μg | Recombinant Human Vimentin | 100μg |
P7262-1mg | Recombinant Human Vimentin | 1mg |
- | 說明書 | 1份 |
保存條件:
???????-20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會引起部分失活,所以首次配制成相應濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液),須分裝后-20℃或更低溫度凍存,以避免反復凍融。
注意事項:
???????由于有些塑料管壁對某些蛋白有較強的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點,使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
???????本產(chǎn)品僅限于專業(yè)人員的科學研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
???????為了您的安全和健康,請穿實驗服并戴一次性手套操作。
成立日期 | 2007-07-19 (18年) | 注冊資本 | 1000.000000萬人民幣 |
員工人數(shù) | 500人以上 | 年營業(yè)額 | ¥ 500萬-1000萬 |
主營行業(yè) | 生化試劑,核苷,核苷酸,寡核苷酸,抗體,蛋白組學,生物活性小分子 | 經(jīng)營模式 | 試劑 |
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