產(chǎn)品編號(hào)
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價(jià)格
AF0291
Stat6 Rabbit Polyclonal Antibody
50μl
412.00元
來(lái)源
用途
交叉反應(yīng)性
分子量
Rabbit
WB, FC, IHC, ICC, IF
H
94KDa
??????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
??????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
??????配套提供了Western一抗稀釋液,可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
??????建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整):
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:1000
-
1:200
1:200
1:200
1:100-1:200
-
-
抗體詳細(xì)信息如下:
Name
Stat6 Rabbit Polyclonal Antibody
Category
Polyclonal antibody(pAb); Primary antibody
Purification
Peptide affinity purified
Immunogen
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human STAT6.
Synonyms
12S1644; IL-4 Stat; IL-4-STAT; Signal transducer and activator of transcription 6; signal transducer and activator of transcription 6; interleukin-4 induced; STAT; interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT
Category
Immunology and Inflammation
Background
In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein STAT6 plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2), expression of cell surface markers, and class switch of immunoglobulins.
包裝清單:
產(chǎn)品編號(hào)
產(chǎn)品名稱
包裝
AF0291
Stat6 Rabbit Polyclonal Antibody
50μl
保存條件:
??????Stat6 Rabbit Polyclonal Antibody -20℃保存,Western一抗稀釋液-20℃或4℃保存,一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存,長(zhǎng)期不使用可以考慮-20℃保存,但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
??????如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn),請(qǐng)注意回收使用過(guò)的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體,包括已經(jīng)使用過(guò)的稀釋抗體,請(qǐng)4℃保存。
??????回收后重復(fù)使用的抗體,使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象,可以10,000g離心1-3分鐘,取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況,則可以考慮廢棄該抗體。
??????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得的檢測(cè)效果,請(qǐng)考慮使用上述檢測(cè)專用的一抗稀釋液。
??????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
??????為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。