基于液滴的微流體技術(shù)是一種新興的強(qiáng)大工具，適用于dPCR或單細(xì)胞分析等生物應(yīng)用。作為微反應(yīng)器的液滴，可以在微流控芯片中產(chǎn)生，具有非常高的吞吐量，可在較短的時(shí)間內(nèi)進(jìn)行大量的平行實(shí)驗(yàn)。

氟化油通常用作這類應(yīng)用的連續(xù)相，因?yàn)槠淠軌蛉芙夂洼斔脱鯕猓⑶也蝗芙獯蠖鄶?shù)化學(xué)和生化化學(xué)物。

FluoSurf 是一種生物相容性氟化表面活性劑，專為生產(chǎn)單分散油包水液滴而設(shè)計(jì)和優(yōu)化。FluoSurf 可確保液滴的長(zhǎng)期穩(wěn)定性（即使在極端條件下也是如此）。

優(yōu)勢(shì)

● 穩(wěn)定性

   即使在dPCR或長(zhǎng)期細(xì)胞培養(yǎng)實(shí)驗(yàn)等具有挑戰(zhàn)性的條件下，也能實(shí)現(xiàn)單分散液滴群體和低液滴融合。

● 生物相容性

   經(jīng)細(xì)胞檢測(cè)驗(yàn)證的生物相容性，適用于生物實(shí)體。

● 批量生產(chǎn)

   我們的大批量生產(chǎn)能力使我們能夠滿足您的所有需求

● 較高純度

   在制造加工過(guò)程中去除雜質(zhì)

● 泄露控制：液滴內(nèi)的分散相物質(zhì)較少量的滲透到液滴外部

● 批次間重現(xiàn)性

   每個(gè)批次都遵循嚴(yán)格的質(zhì)量控制分析，通過(guò)驗(yàn)證結(jié)構(gòu)和微流體性能來(lái)確保一致性。

FluoSurf 在高溫處理（例如PCR的加熱循環(huán)）后仍保證液滴穩(wěn)定性和群體單分散性。

應(yīng)用領(lǐng)域

● 數(shù)字液滴PCR

   基于對(duì)液滴內(nèi)大量分隔PCR反應(yīng)的統(tǒng)計(jì)分析的超靈敏核酸定量方法

● 單細(xì)胞分析

   在受控環(huán)境下處理和分析單分散液滴群體中的分離細(xì)胞

● 篩選

   在微滴內(nèi)以高通量對(duì)細(xì)胞、基因或生物大分子進(jìn)行受控操作、分析和分選。

● 合成生物學(xué)

   使用微液滴作為細(xì)胞大小的隔間來(lái)模擬代謝反應(yīng)。

● 控制化學(xué)和生物反應(yīng)

   微流體工具專門設(shè)計(jì)用于增強(qiáng)對(duì)封裝在液滴內(nèi)的反應(yīng)的控制。

規(guī)格參數(shù)

案例1: 液滴產(chǎn)生和熱循環(huán)對(duì)尺寸分布的影響

實(shí)驗(yàn)條件：氟油流速為5μL/min，水相流速為1.66μL/min。

4w/w%表面活性劑HFE7500

熱循環(huán)條件：30個(gè)循環(huán)

95℃駐留30s

55℃駐留1min

72℃駐留5min

40℃駐留1min

熱循環(huán)前

熱循環(huán)后

FluoSurf在熱循環(huán)期間允許液滴具有良好的穩(wěn)定性

使用FluoSurf表面活性劑，熱循環(huán)對(duì)液滴尺寸分布的影響十分有限。

案例2：保留性能

熒光素100μM的全/空液滴平均強(qiáng)度的演變

FluoSurf限制液滴之間的交換（4天后少于10%的熒光素交換）

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