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A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū),A549[A-549]
  • A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū),A549[A-549]

A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū)

價(jià)格 詢價(jià)
包裝 1000000細(xì)胞數(shù) 2000000細(xì)胞數(shù)
最小起訂量 1000000細(xì)胞數(shù)
發(fā)貨地 上海
更新日期 2025-02-23
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產(chǎn)品詳情

中文名稱:A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū)英文名稱:A549[A-549]
品牌: ATCC\RCB等產(chǎn)地: 國(guó)外
保存條件: 常溫培養(yǎng)或液氮凍存純度規(guī)格: A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū)
產(chǎn)品類別: 化學(xué)試劑
種屬: 詳見(jiàn)產(chǎn)品資料組織: 詳見(jiàn)產(chǎn)品資料
細(xì)胞系: 詳見(jiàn)產(chǎn)品資料細(xì)胞形態(tài): 詳見(jiàn)產(chǎn)品資料
生長(zhǎng)狀態(tài): 詳見(jiàn)產(chǎn)品資料靶點(diǎn): 詳見(jiàn)產(chǎn)品資料
應(yīng)用: 詳見(jiàn)產(chǎn)品資料
2025-02-23 A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū) A549[A-549] 1000000細(xì)胞數(shù)/RMB;2000000細(xì)胞數(shù)/RMB ATCC\RCB等 國(guó)外 常溫培養(yǎng)或液氮凍存 A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū) 化學(xué)試劑

"A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū)

傳代比例:1:2-1:4(首次傳代建議1:2)

生長(zhǎng)特性:貼壁生長(zhǎng)

貼壁細(xì)胞消化傳代時(shí)通常采用兩種方法:一、加入胰酶等細(xì)胞脫落后,再加培養(yǎng)基中止胰酶作用,離心傳代;二、加入胰酶后,鏡下觀察待細(xì)胞始脫落時(shí),棄胰酶,加培養(yǎng)分瓶。但前者太麻煩,而后者有可能對(duì)細(xì)胞施加胰酶選擇,因?yàn)榭偸琴N壁不牢的細(xì)胞先脫落,對(duì)腫瘤細(xì)胞來(lái)說(shuō),這部分細(xì)胞有可能是惡性程度較GAO的細(xì)胞亞群。一種簡(jiǎn)單的消化傳代方法。加入PBS洗去血清或加入胰酶先中和血清的作用(30s),棄之,再加入適量胰酶作用10s-40s(根據(jù)細(xì)胞消化的難易程度),棄之,這樣依賴殘余的胰酶就可將細(xì)胞消化單細(xì)胞。對(duì)于較難消化的細(xì)胞,可以用2%利多卡因消化5-8分鐘,然后再棄去,加培養(yǎng)基吹打也可以,對(duì)細(xì)胞的影響不大。不用PBS也不用Hanks洗,只要把舊培養(yǎng)吸的干凈一點(diǎn),直接加酶消化應(yīng)該不會(huì)有什么問(wèn)題。棄培養(yǎng)后,用0.04%的EDA沖洗一次,再用1/4v的0.04%的EDA室溫孵育5min,棄取大部分EDA,加入與剩余EDA等量的胰酶(預(yù)熱)總體積1/10v。消化到有細(xì)胞脫落。不過(guò)有人說(shuō)EDA對(duì)細(xì)胞不HAO,有證據(jù)嗎?培養(yǎng)的BASMC:倒掉舊培養(yǎng)加入少量胰酶沖一下,倒掉再加入0.125-0.25%胰酶約6-10滴或1ml(25ml bole)消化再加入適量新培養(yǎng)基中和,并分瓶這種方法簡(jiǎn)單、省事;效果很HAO并且不損失細(xì)胞!

換液周期:每周2-3次

CHP-126 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:OKT 3細(xì)胞、CCC-HIE-2細(xì)胞、HOC1細(xì)胞

H-1238 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:ARH77細(xì)胞、Anip[973]細(xì)胞、THLE-3細(xì)胞

SKMES1 Cells;背景說(shuō)明:源于一位65歲患有肺鱗狀細(xì)胞癌的白人男性,自轉(zhuǎn)移性胸腔積液分離而來(lái)。;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:MG63細(xì)胞、NU-GC-4細(xì)胞、SW-982細(xì)胞

A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū)

背景信息:最初分離自一名58歲白人男性的非小細(xì)胞肺癌組織,于1972年由科學(xué)家D. J. Giard等人培養(yǎng)成功。能通過(guò)胞苷二磷酸膽堿途徑合成含有高含量不飽和脂肪酸的卵磷脂。

細(xì)胞系的選擇需要考慮到細(xì)胞系的功能特點(diǎn)、生長(zhǎng)速率、鋪板效率、生長(zhǎng)條件和生長(zhǎng)特征、克隆效率、培養(yǎng)方式等因素,如果您想高產(chǎn)量表達(dá)重組蛋白,您可以選擇可以懸浮生長(zhǎng)的快速生長(zhǎng)細(xì)胞系。細(xì)胞培養(yǎng)的操作步驟主要包括傳代、換液、凍存和復(fù)蘇。這些步驟確保了細(xì)胞能夠在實(shí)驗(yàn)室環(huán)境中長(zhǎng)期存活并繼續(xù)增殖。傳代是將細(xì)胞從一個(gè)容器轉(zhuǎn)移到另一個(gè)容器的過(guò)程,以擴(kuò)大細(xì)胞數(shù)量;換液是為了清除代謝廢物并補(bǔ)充新鮮培養(yǎng)基;凍存則是為了長(zhǎng)期保存細(xì)胞,而復(fù)蘇則是重新激活冷凍保存的細(xì)胞使其恢復(fù)正常生長(zhǎng)。

產(chǎn)品包裝:復(fù)蘇發(fā)貨:T25培養(yǎng)瓶(一瓶)或凍存發(fā)貨:1ml凍存管(兩支)

來(lái)源說(shuō)明:細(xì)胞主要來(lái)源ATCC、ECACC、DSMZ、RIKEN等細(xì)胞庫(kù)

AN3CA Cells;背景說(shuō)明:AN3CA細(xì)胞建系于1964年。它衍生于子宮內(nèi)膜癌患者淋巴結(jié)轉(zhuǎn)移組織,具有癌細(xì)胞的基本特性,能在體外長(zhǎng)期傳代培養(yǎng),接種實(shí)驗(yàn)動(dòng)物產(chǎn)生明顯腫瘤。但細(xì)胞的生物學(xué)特性及超微結(jié)構(gòu)尚未深入研究,僅發(fā)現(xiàn)該細(xì)胞系促黑激素合成為陰性。細(xì)胞常用于人子宮內(nèi)膜癌細(xì)胞生物學(xué)及其相關(guān)特性研究。;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:MB 157細(xì)胞、C6661細(xì)胞、Rat podocyte細(xì)胞

GM00346B Cells;背景說(shuō)明:皮下結(jié)締組織;自發(fā)永生;雄性;C3H/An;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:ReNcell CX細(xì)胞、RCC10 RGB細(xì)胞、TR-146細(xì)胞

Hs 611.T Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2傳代;每周換液2-3次。;生長(zhǎng)特性:混合型;形態(tài)特性:淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有:PE/CA-PJ34 (clone C12)細(xì)胞、P3/X63-Ag8細(xì)胞、HPAF/CD18細(xì)胞

WC00044 Cells;背景說(shuō)明:黑色素瘤;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:HEK 293 EBNA細(xì)胞、Stanford University-Diffuse Histiocytic Lymphoma-16細(xì)胞、MDA-175細(xì)胞

A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū)

物種來(lái)源:人源、鼠源等其它物種來(lái)源

形態(tài)特性:上皮細(xì)胞樣

其實(shí)絕大部分細(xì)胞消化的時(shí)候是只要用胰酶潤(rùn)洗一遍即可,吸去胰酶后,殘留的那些無(wú)法計(jì)算體積的附著在細(xì)胞表面的微量胰酶在37度一般不到2min足夠消化細(xì)胞(絕大部分1min不到)。對(duì)于這些細(xì)胞原則上不要用胰酶孵育細(xì)胞,連續(xù)這樣傳代,對(duì)細(xì)胞傷害很大。簡(jiǎn)單的程序是PBS潤(rùn)洗吸去,胰酶潤(rùn)洗吸去,然后37度消化;什么算是消化HAO了呢?不是細(xì)胞全部成間隔分布很離散的單個(gè)圓形才算消化HAO了,一般你肉眼觀察貼壁細(xì)胞層,只要能移動(dòng)了,多半呈沙壯移動(dòng),其實(shí)已經(jīng)可以了,很多人喜歡把細(xì)胞消化或者吹打成完全分離細(xì)胞,這是沒(méi)有必要的。一般能移動(dòng)了,說(shuō)明細(xì)胞與培養(yǎng)基質(zhì)材料的附著已經(jīng)消失了,細(xì)胞之間的附著也已經(jīng)消失了,細(xì)胞已經(jīng)獨(dú)立分布了(雖然沒(méi)有呈現(xiàn)很廣的離散分布)。這個(gè)時(shí)候應(yīng)該停止消化,不要等到看到鏡下所有細(xì)胞都分離得非常HAO,間隙很大,才停止。細(xì)胞就是完全成單個(gè)細(xì)胞懸,之后在貼壁的過(guò)程中仍然會(huì)聚集,這個(gè)是貼壁培養(yǎng)的細(xì)胞,尤其是腫瘤細(xì)胞的一個(gè)性,無(wú)論死活的細(xì)胞都是如此,你可以嘗試,準(zhǔn)備100%的單個(gè)細(xì)胞懸,貼壁后觀察細(xì)胞,仍然是幾個(gè)幾個(gè)細(xì)胞聚集在一起。一些懸浮培養(yǎng)細(xì)胞也是如此,容易聚集,不要去嘗試過(guò)幾個(gè)小時(shí)就拿出來(lái)吹打成單細(xì)胞懸(不要笑,這個(gè)是初養(yǎng)懸浮細(xì)胞的人常犯的錯(cuò)誤,以為懸浮培養(yǎng)就是一個(gè)一個(gè)分開(kāi))。細(xì)胞只要能從基質(zhì)上脫離下來(lái),這個(gè)時(shí)候即使是成片的(比如Calu-3細(xì)胞),吹打不超過(guò)20次后(一般10次即可),成小規(guī)模聚集(10個(gè)細(xì)胞左右),是正常的,不要試圖再去延長(zhǎng)消化時(shí)間,或者像有的同學(xué)那樣吹打1h,等待單細(xì)胞懸出現(xiàn)。

KTCTL140 Cells;背景說(shuō)明:腎透明細(xì)胞癌;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:H-2009細(xì)胞、Evsa-T細(xì)胞、COLO 678細(xì)胞

RASMC Cells;背景說(shuō)明:主動(dòng)脈;平滑肌 Cells;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:OCI-Ly8細(xì)胞、TPC-1細(xì)胞、WSU-DLCL-2細(xì)胞

BHK Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2傳代,每周換液1-2次。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:成纖維細(xì)胞樣;相關(guān)產(chǎn)品有:TEC細(xì)胞、I90細(xì)胞、H2085細(xì)胞

alpha TC1 clone 6 Cells;背景說(shuō)明:胰島素瘤;a細(xì)胞;C57BL/6xDBA/2;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:Leukemic 1210細(xì)胞、COLO-320-HSR細(xì)胞、H19-7細(xì)胞

COLO 357 Cells;背景說(shuō)明:胰腺癌;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:MB-49細(xì)胞、PC 61-5-3細(xì)胞、AR42J細(xì)胞

D283 Med Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:每周換液2-3次。;生長(zhǎng)特性:懸浮細(xì)胞的多細(xì)胞聚集體,和一些貼壁 Cells;形態(tài)特性:上皮細(xì)胞;相關(guān)產(chǎn)品有:253JB-V細(xì)胞、L 428細(xì)胞、BC3H1細(xì)胞

GM02132C Cells;背景說(shuō)明:來(lái)源于一位61歲的男性漿細(xì)胞瘤患者;可產(chǎn)生免疫球蛋白輕鏈,未檢測(cè)到重鏈。;傳代方法:按1:2傳代,5-6小時(shí)可以看到細(xì)胞分裂;生長(zhǎng)特性:懸浮生長(zhǎng);形態(tài)特性:淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有:HR-8348細(xì)胞、GLC82細(xì)胞、MC57G細(xì)胞

HCC-38 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2—1:4傳代,每周換液2—3次;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:KLM1細(xì)胞、KE39細(xì)胞、SW-756細(xì)胞

SK.MEL.5 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:3-1:6傳代,2-3天換液1次。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:星形的;相關(guān)產(chǎn)品有:Co 115細(xì)胞、HCT.116細(xì)胞、PLA801C細(xì)胞

MB-49 Cells;背景說(shuō)明:膀胱癌;雄性;C57BL/Icrfa(t);傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:HT29細(xì)胞、KOPN-8細(xì)胞、UC-3細(xì)胞

HcaF Cells;背景說(shuō)明:肝癌;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:H8細(xì)胞、YD-15細(xì)胞、RT-112細(xì)胞

MILE SVEN1 Cells;背景說(shuō)明:MS1是1994年建株的胰島內(nèi)皮細(xì)胞株。原代培養(yǎng)的胰島內(nèi)皮細(xì)胞用抗G418的溫度敏感型SV40大T抗原(tsA-58-3)轉(zhuǎn)染??剐钥寺∮每寺…h(huán)分離,并篩選吸收dil-Ac-LDL的。這株細(xì)胞保留了內(nèi)皮細(xì)胞的許多特性,如吸收乙酰化LDL和表達(dá)八因子相關(guān)抗原及BEGF受體。;傳代方法:消化3-5分鐘。1:2。3天內(nèi)可長(zhǎng)滿。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:3T3-A31細(xì)胞、HuH7細(xì)胞、IFRS1細(xì)胞

NIH3T3 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:MGH-U1細(xì)胞、266 Bl細(xì)胞、TE-4細(xì)胞

293S Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:CL-34細(xì)胞、LC1sq細(xì)胞、RBL-2H3細(xì)胞

HCO Cells;背景說(shuō)明:顱骨;成骨 Cells;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:SNU368細(xì)胞、Wien 133細(xì)胞、SJSA-1細(xì)胞

SKN-AS Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:5-1:10傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:NCI-H1238細(xì)胞、Glioma-261細(xì)胞、Earle's L cells細(xì)胞

IOSE 80 Cells;背景說(shuō)明:卵巢;上皮細(xì)胞;SV40轉(zhuǎn)化;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:H2330細(xì)胞、NALM-6細(xì)胞、FHC細(xì)胞

A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū)

Abcam HEK293 DIAPH1 KO Cells(提供STR鑒定圖譜)

AG08615 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line KST109 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line XB748 Cells(提供STR鑒定圖譜)

BP8 Cells(提供STR鑒定圖譜)

CoE 45 Cells(提供STR鑒定圖譜)

DA03814 Cells(提供STR鑒定圖譜)

DA05324 Cells(提供STR鑒定圖譜)

GENEA057 Cells(提供STR鑒定圖譜)

OCI-Ly 8 Cells;背景說(shuō)明:彌漫大B淋巴瘤;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:懸浮;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:K562/ADP細(xì)胞、MOLT3細(xì)胞、MDA-MB-436細(xì)胞

Hs-27 Cells;背景說(shuō)明:包皮;成纖維細(xì)胞;男性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:SKNBE2細(xì)胞、NTERA2-D1細(xì)胞、SNU5細(xì)胞

NCI-H64 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:HepaRG細(xì)胞、D-324 Med細(xì)胞、McA-RH7777細(xì)胞

SJSA-1 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:5-1:10傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:成纖維細(xì)胞;相關(guān)產(chǎn)品有:PECAPJ34細(xì)胞、HAC-84細(xì)胞、MDA.MB.435細(xì)胞

CHP 126 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:H-23細(xì)胞、OVCAR.3細(xì)胞、Cates-1B細(xì)胞

KM12 SM Cells;背景說(shuō)明:結(jié)腸癌;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:Madin Darby Bovine Kidney細(xì)胞、SCH細(xì)胞、GAK細(xì)胞

1B3-18 Cells(提供STR鑒定圖譜)

RIN Cl-5F Cells;背景說(shuō)明:胰島β細(xì)胞瘤;雄性;NEDH;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:SNB19細(xì)胞、HCC9204細(xì)胞、GM15452細(xì)胞

GDM1 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:2-3天換液1次。;生長(zhǎng)特性:懸浮生長(zhǎng);形態(tài)特性:淋巴母細(xì)胞樣 ;相關(guān)產(chǎn)品有:Jurkat-FHCRC細(xì)胞、WPMY-1細(xì)胞、MKN-1細(xì)胞

HCe-8693 Cells;背景說(shuō)明:盲腸腺癌;男性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:MOLM-16細(xì)胞、UACC893細(xì)胞、OCIAML5細(xì)胞

OCI-AML-5 Cells;背景說(shuō)明:急性髓系白血病細(xì)胞;男性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:懸浮;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:SNU398細(xì)胞、16HBE細(xì)胞、SK-N-BE(2C)細(xì)胞

HFT 8810 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:CAL-85-1細(xì)胞、MPC 11細(xì)胞、HEC-151細(xì)胞

RKOAS451 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:T2(174 x CEM.T2)細(xì)胞、H-2141細(xì)胞、H-820細(xì)胞

HMO6 Cells;背景說(shuō)明:小膠質(zhì) Cells;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:NG 108-15細(xì)胞、CNE2Z細(xì)胞、H-378細(xì)胞

HEK293 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:U-343-MG細(xì)胞、HIMEC細(xì)胞、Murine Carcinoma-38細(xì)胞

GM10018 Cells(提供STR鑒定圖譜)

HAP1 BTBD9 (-) Cells(提供STR鑒定圖譜)

Acanthosis Nigricans 3rd attempt-CArcinoma Cells;背景說(shuō)明:AN3CA細(xì)胞建系于1964年。它衍生于子宮內(nèi)膜癌患者淋巴結(jié)轉(zhuǎn)移組織,具有癌細(xì)胞的基本特性,能在體外長(zhǎng)期傳代培養(yǎng),接種實(shí)驗(yàn)動(dòng)物產(chǎn)生明顯腫瘤。但細(xì)胞的生物學(xué)特性及超微結(jié)構(gòu)尚未深入研究,僅發(fā)現(xiàn)該細(xì)胞系促黑激素合成為陰性。細(xì)胞常用于人子宮內(nèi)膜癌細(xì)胞生物學(xué)及其相關(guān)特性研究。;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:U251細(xì)胞、SNU1040細(xì)胞、MLFC細(xì)胞

PL 45 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:RK 13細(xì)胞、HC-11細(xì)胞、Human Embryo Lung-1細(xì)胞

OVCA-433 Cells;背景說(shuō)明:卵巢癌;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:HOS細(xì)胞、NCC-IT細(xì)胞、Bowes melanoma cells細(xì)胞

MDA-MB435 Cells;背景說(shuō)明:乳腺癌;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:BHK-21細(xì)胞、Me-Wo細(xì)胞、NCI-H1238細(xì)胞

NSI/1-Ag4-1 Cells;背景說(shuō)明:這是P3X63Ag8(ATCCTIB-9)的一個(gè)不分泌克隆。Kappa鏈合成了但不分泌。能抗0.1mM8-氮雜鳥(niǎo)嘌呤但不能在HAT培養(yǎng)基中生長(zhǎng)。據(jù)報(bào)道它是由于缺失了3-酮類固醇還原酶活性的膽固醇營(yíng)養(yǎng)缺陷型。檢測(cè)表明肢骨發(fā)育畸形病毒(鼠痘)陰性。;傳代方法:1:2傳代,3天內(nèi)可長(zhǎng)滿。;生長(zhǎng)特性:懸浮生長(zhǎng);形態(tài)特性:淋巴母細(xì)胞;相關(guān)產(chǎn)品有:NCI-H2066細(xì)胞、Nthy-ori 3.1細(xì)胞、alpha TC1 clone 6細(xì)胞

ChaGo-K1 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:4-1:8?jìng)鞔?;每周換液2次。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮細(xì)胞;相關(guān)產(chǎn)品有:U-118 MG細(xì)胞、MDA-330細(xì)胞、alpha TC1.6細(xì)胞

YES-2 Cells;背景說(shuō)明:食管鱗癌;男性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:NCI-H889細(xì)胞、OCI-LY-18細(xì)胞、BNL 1MEA.7R.1細(xì)胞

MC-26 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:HCC-1428細(xì)胞、C3A細(xì)胞、Hs 683細(xì)胞

HG03466 Cells(提供STR鑒定圖譜)

ID00085 Cells(提供STR鑒定圖譜)

LOPRA-1/4 Cells(提供STR鑒定圖譜)

NCI-H69/IFN-gamma Cells(提供STR鑒定圖譜)

PathHunter U2OS HTR1F beta-arrestin Cells(提供STR鑒定圖譜)

Ubigene HeLa VIPR2 KO Cells(提供STR鑒定圖譜)

XPHM12BE Cells(提供STR鑒定圖譜)

hFIB2-iPS5 Cells(提供STR鑒定圖譜)

E304 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:SR-786細(xì)胞、3LL細(xì)胞、U138細(xì)胞

WM 266-4 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:CMT 167細(xì)胞、SNU484細(xì)胞、CTLL-2細(xì)胞

NuTu-19 Cells;背景說(shuō)明:卵巢癌;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:SUP-B15細(xì)胞、WI 38細(xì)胞、Tb 1-Lu細(xì)胞

LN 229 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:4-1:6傳代;每周換液2-3次;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮細(xì)胞;相關(guān)產(chǎn)品有:RBMVEC細(xì)胞、SUM 52PE細(xì)胞、COLO-679細(xì)胞

AML 12 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:Kit225 K6細(xì)胞、NCI-H1882細(xì)胞、NCIN87細(xì)胞

AML 12 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:Kit225 K6細(xì)胞、NCI-H1882細(xì)胞、NCIN87細(xì)胞

Lilly Laboratories Cell-Porcine Kidney 1 Cells;背景說(shuō)明:腎;自發(fā)永生;Hampshire;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:LIM 1215細(xì)胞、HMC-1細(xì)胞、HuH28細(xì)胞

Mv1.Lu Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:MDA-MB-361細(xì)胞、H1975細(xì)胞、P3X63細(xì)胞

KALS-1 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:多邊形;相關(guān)產(chǎn)品有:RA 1細(xì)胞、SUDHL1細(xì)胞、OVCA433細(xì)胞

MC57 Cells;背景說(shuō)明:纖維肉瘤;C57BL/6J;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:NB19-RIKEN細(xì)胞、Moorfields/Institute of Ophthalmology-Muller 1細(xì)胞、NPA細(xì)胞

AG 9 Cells;背景說(shuō)明:皮下結(jié)締組織;自發(fā)永生;雄性;C3H/An;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:Ramos-RA1細(xì)胞、IAR20細(xì)胞、NCIH345細(xì)胞

Alexander Cells;背景說(shuō)明:該細(xì)胞系分泌乙肝病毒表面抗原(HBsAg)。 此細(xì)胞系原先被支原體污染,后用BM-cycline去除支原體;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:IPLB-SF-21-AE細(xì)胞、95D細(xì)胞、Sp2/0-Ag-14細(xì)胞

THC-8307 Cells;背景說(shuō)明:高分化結(jié)腸癌;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:LS 123細(xì)胞、SW-403細(xì)胞、Medical Research Council cell strain-9細(xì)胞

Kit225/K6 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:J111細(xì)胞、GM00215細(xì)胞、OCI-AML4細(xì)胞

PANC-1 Cells;背景說(shuō)明:這株人胰腺癌細(xì)胞株源自于胰腺癌導(dǎo)管細(xì)胞,其倍增時(shí)間為52小時(shí)。染色體研究表明,該細(xì)胞染色體眾數(shù)為63,包括3個(gè)獨(dú)特標(biāo)記的染色體和1個(gè)小環(huán)狀染色體。該細(xì)胞的生長(zhǎng)可被1unit/ml的左旋天冬酰胺酶抑制;能在軟瓊脂上生長(zhǎng);能在裸鼠上成瘤。;傳代方法:1:2-1:4傳代;每周2-3次。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;多角形;相關(guān)產(chǎn)品有:HIEC-6細(xì)胞、HIT T15細(xì)胞、Leghorn Male Hepatoma cell line細(xì)胞

SBB-W1 Cells(提供STR鑒定圖譜)

Hs-578Bst Cells;背景說(shuō)明:乳腺 Cells;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:MN 60細(xì)胞、P3-Jiyoye細(xì)胞、UMR-106細(xì)胞

Japanese Tissue Culture-39 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:消化3-5分鐘。1:2。3天內(nèi)可長(zhǎng)滿。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:HCT-8細(xì)胞、Human Embryo Lung-1細(xì)胞、LLC-PK(1)細(xì)胞

Homo sapiens No. 578, tumor cells Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:HS852.T細(xì)胞、NCI-H146細(xì)胞、Giant Cell Tumor細(xì)胞

WM-451Lu Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁或懸浮,詳見(jiàn)產(chǎn)品說(shuō)明書(shū)部分;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:MD Anderson-Metastatic Breast-453細(xì)胞、H-1666細(xì)胞、LN-18細(xì)胞

TFK-1 Cells;背景說(shuō)明:膽管癌;男性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:PNT1A細(xì)胞、MES-SA-Dx5細(xì)胞、MDA PCa 2b細(xì)胞

Moorfields/Institute of Ophthalmology-Muller 1 Cells;背景說(shuō)明:視網(wǎng)膜Muller細(xì)胞;自發(fā)永生;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長(zhǎng)特性:貼壁;形態(tài)特性:詳見(jiàn)產(chǎn)品說(shuō)明書(shū);相關(guān)產(chǎn)品有:PC 12細(xì)胞、NCI-H810細(xì)胞、Hs 578.T細(xì)胞

CHO cell clone K1 Cells;背景說(shuō)明:1957年,PuckTT從成年中國(guó)倉(cāng)鼠卵巢的活檢組織建立了CHO細(xì)胞,CHO-K1是CHO的一個(gè)亞克隆。CHO-K1的生長(zhǎng)需要脯酸。;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:HCC-95細(xì)胞、SKNSH細(xì)胞、HCC9724細(xì)胞

TE-1 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:消化3-5分鐘。1:2。3天內(nèi)可長(zhǎng)滿。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:OC316細(xì)胞、Sf-21細(xì)胞、HCC-1395細(xì)胞

SW 579 Cells;背景說(shuō)明:在裸鼠中成瘤(產(chǎn)生三級(jí)惡性紡錘狀巨細(xì)胞瘤)。 ;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮細(xì)胞樣;相關(guān)產(chǎn)品有:ATN-1細(xì)胞、WM 115細(xì)胞、EPC細(xì)胞

786-0 Cells;背景說(shuō)明:該細(xì)胞源自一位原發(fā)性腎透明細(xì)胞癌患者。該細(xì)胞有微絨毛和橋粒,能在軟瓊脂上生長(zhǎng)。此細(xì)胞生成一種PTH(甲狀旁腺素)樣的多肽,與乳癌和肺癌中生成的肽相似,其N端序列與PTH相似,具有PTH樣活性,分子量為6000D。;傳代方法:1:2傳代;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:NCIH716細(xì)胞、SNB.19細(xì)胞、H810細(xì)胞

Caco2BBe Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:6—1:10傳代,每周換液2次;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮細(xì)胞;相關(guān)產(chǎn)品有:Tn-5B1-4細(xì)胞、SK-MEL-31細(xì)胞、H841細(xì)胞

H-1395 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:2傳代;5-6天傳代一次。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:上皮樣;多角形;相關(guān)產(chǎn)品有:A549-Taxol細(xì)胞、PC-3M-IE8細(xì)胞、NCI-522細(xì)胞

CHP-212 Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:10 1:50每2 - 3周;每周換液2-3次。;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:成神經(jīng)細(xì)胞;相關(guān)產(chǎn)品有:HOS細(xì)胞、Colon-38細(xì)胞、RBE4細(xì)胞

RTE Cells;背景說(shuō)明:詳見(jiàn)相關(guān)文獻(xiàn)介紹;傳代方法:1:3傳代,3-4天換液一次;生長(zhǎng)特性:貼壁生長(zhǎng);形態(tài)特性:多角;相關(guān)產(chǎn)品有:Vero細(xì)胞、NCIH2023細(xì)胞、GM06141細(xì)胞

A549[A-549]人非小細(xì)胞肺癌復(fù)蘇細(xì)胞保種中心|帶STR證書(shū)

BayGenomics ES cell line RRR235 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line YHD208 Cells(提供STR鑒定圖譜)

HT22 TR/TO-rKlf9 clone 2.1 Cells(提供STR鑒定圖譜)

PCRP-HEY2-1H7 Cells(提供STR鑒定圖譜)

ERC-13 Cells(提供STR鑒定圖譜)

HPS2724 Cells(提供STR鑒定圖譜)

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Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

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Cell line A549: a model system for the study of alveolar type II cell function.

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Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.

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Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces.

Proc. Natl. Acad. Sci. U.S.A. 76:1288-1292(1979)


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Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains.

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Day R.S. 3rd, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Mattern M.R.

Human tumor cell strains defective in the repair of alkylation damage.

Carcinogenesis 1:21-32(1980)


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High levels of intracellular bombesin characterize human small-cell lung carcinoma.

Science 214:1246-1248(1981)


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Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.

J. Natl. Cancer Inst. 66:239-247(1981)


PubMed=7459858

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Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.

Cancer Res. 41:1165-1170(1981)


PubMed=6954533; DOI=10.1073/pnas.79.7.2194; PMCID=PMC346157

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Differential expression of the amv gene in human hematopoietic cells.

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PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222

Hay R.J., Williams C.D., Macy M.L., Lavappa K.S.

Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.

Am. Rev. Respir. Dis. 125:222-232(1982)


PubMed=6825208; DOI=10.1093/carcin/4.2.199

Yarosh D.B., Foote R.S., Mitra S., Day R.S. 3rd

Repair of O6-methylguanine in DNA by demethylation is lacking in Mer- human tumor cell strains.

Carcinogenesis 4:199-205(1983)


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Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.

J. Natl. Cancer Inst. 73:801-807(1984)


PubMed=6500159; DOI=10.1159/000163283

Gershwin M.E., Lentz D., Owens R.B.

Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.

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Fogh J.

Human tumor lines for cancer research.

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Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.

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Four human carcinoma cell lines with novel mutations in position 12 of c-K-ras oncogene.

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Evidence for thromboxane biosynthesis in established cell lines derived from human lung adenocarcinomas.

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Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

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p53 mutations, ras mutations, and p53-heat shock 70 protein complexes in human lung carcinoma cell lines.

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Monks A., Scudiero D.A., Skehan P., Shoemaker R.H., Paull K.D., Vistica D.T., Hose C.D., Langley J., Cronise P., Vaigro-Wolff A., Gray-Goodrich M., Campbell H., Mayo J.G., Boyd M.R.

Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.

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Kitata R.B., Dimayacyac-Esleta B.R.T., Choong W.-K., Tsai C.-F., Lin T.-D., Tsou C.-C., Weng S.-H., Chen Y.-J., Yang P.-C., Arco S.D., Nesvizhskii A.I., Sung T.-Y., Chen Y.-J.

Mining missing membrane proteins by high-pH reverse-phase stagetip fractionation and multiple reaction monitoring mass spectrometry.

J. Proteome Res. 14:3658-3669(2015)


PubMed=26554430; DOI=10.1021/acs.analchem.5b03639

Dimayacyac-Esleta B.R.T., Tsai C.-F., Kitata R.B., Lin P.-Y., Choong W.-K., Lin T.-D., Wang Y.-T., Weng S.-H., Yang P.-C., Arco S.D., Sung T.-Y., Chen Y.-J.

Rapid high-pH reverse phase stagetip for sensitive small-scale membrane proteomic profiling.

Anal. Chem. 87:12016-12023(2015)


PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878

Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Genome Med. 7:118.1-118.7(2015)


PubMed=27377824; DOI=10.1038/sdata.2016.52; PMCID=PMC4932877

Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.

Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.

Sci. Data 3:160052-160052(2016)


PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Miroo T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)


PubMed=27807467; DOI=10.1186/s13100-016-0078-4; PMCID=PMC5087121

Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.

A map of mobile DNA insertions in the NCI-60 human cancer cell panel.

Mob. DNA 7:20.1-20.11(2016)


PubMed=28114404; DOI=10.1371/journal.pone.0170609; PMCID=PMC5256872

Fang Y.-N., Zhang C., Wu T., Wang Q., Liu J.-H., Dai P.-G.

Transcriptome sequencing reveals key pathways and genes associated with cisplatin resistance in lung adenocarcinoma A549 cells.

PLoS ONE 12:E0170609-E0170609(2017)


PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076

Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)


PubMed=28408844; DOI=10.2147/OTT.S128416; PMCID=PMC5384690

Townsend M.H., Anderson M.D., Weagel E.G., Velazquez E.J., Weber K.S., Robison R.A., O'Neill K.L.

Non-small-cell lung cancer cell lines A549 and NCI-H460 express hypoxanthine guanine phosphoribosyltransferase on the plasma membrane.

Onco Targets Ther. 10:1921-1932(2017)


PubMed=28601559; DOI=10.1016/j.cels.2017.05.009; PMCID=PMC5493283

Bekker-Jensen D.B., Kelstrup C.D., Batth T.S., Larsen S.C., Haldrup C., Bramsen J.B., Sorensen K.D., Hoyer S., Orntoft T.F., Lindbjerg Andersen C., Nielsen M.L., Olsen J.V.

An optimized shotgun strategy for the rapid generation of comprehensive human proteomes.

Cell Syst. 4:587-599.e4(2017)


PubMed=28746345; DOI=10.1371/journal.pone.0181081; PMCID=PMC5528889

Sarin N., Engel F., Kalayda G.V., Mannewitz M., Cinatl J. Jr., Rothweiler F., Michaelis M., Saafan H., Ritter C.A., Jaehde U., Frotschl R.

Cisplatin resistance in non-small cell lung cancer cells is associated with an abrogation of cisplatin-induced G2/M cell cycle arrest.

PLoS ONE 12:E0181081-E0181081(2017)


PubMed=29444439; DOI=10.1016/j.celrep.2018.01.051; PMCID=PMC6343826

Yuan T.L., Amzallag A., Bagni R., Yi M., Afghani S., Burgan W., Fer N., Strathern L.A., Powell K., Smith B., Waters A.M., Drubin D.A., Thomson T., Liao R., Greninger P., Stein G.T., Murchie E., Cortez E., Egan R.K., Procter L., Bess M., Cheng K.T., Lee C.-S., Lee L.C., Fellmann C., Stephens R., Luo J., Lowe S.W., Benes C.H., McCormick F.

Differential effector engagement by oncogenic KRAS.

Cell Rep. 22:1889-1902(2018)


PubMed=29457907; DOI=10.1021/acs.jproteome.7b00782

Tomin T., Fritz K., Gindlhuber J., Waldherr L., Pucher B., Thallinger G.G., Nomura D.K., Schittmayer M., Birner-Gruenberger R.

Deletion of adipose triglyceride lipase links triacylglycerol accumulation to a more-aggressive phenotype in A549 lung carcinoma cells.

J. Proteome Res. 17:1415-1425(2018)


PubMed=29468137; DOI=10.5501/wjv.v7.i1.10; PMCID=PMC5807893

Himmelsbach K., Hildt E.

Identification of various cell culture models for the study of Zika virus.

World J. Virol. 7:10-20(2018)


PubMed=29681454; DOI=10.1016/j.cell.2018.03.028; PMCID=PMC5935540

McMillan E.A., Ryu M.-J., Diep C.H., Mendiratta S., Clemenceau J.R., Vaden R.M., Kim J.-H., Motoyaji T., Covington K.R., Peyton M., Huffman K., Wu X.-F., Girard L., Sung Y., Chen P.-H., Mallipeddi P.L., Lee J.Y., Hanson J., Voruganti"


關(guān)鍵字: A549[A-549]人非小細(xì)胞肺癌復(fù)蘇;傳代細(xì)胞;復(fù)蘇細(xì)胞;實(shí)驗(yàn)細(xì)胞;科研細(xì)胞;

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