"HT-29人結(jié)腸癌細(xì)胞全年復(fù)蘇|已有STR圖譜
傳代比例:1:2-1:4(首次傳代建議1:2)
生長特性:貼壁生長
細(xì)胞系的選擇需要考慮到細(xì)胞系的功能特點、生長速率、鋪板效率、生長條件和生長特征、克隆效率、培養(yǎng)方式等因素,如果您想高產(chǎn)量表達重組蛋白,您可以選擇可以懸浮生長的快速生長細(xì)胞系。細(xì)胞培養(yǎng)的操作步驟主要包括傳代、換液、凍存和復(fù)蘇。這些步驟確保了細(xì)胞能夠在實驗室環(huán)境中長期存活并繼續(xù)增殖。傳代是將細(xì)胞從一個容器轉(zhuǎn)移到另一個容器的過程,以擴大細(xì)胞數(shù)量;換液是為了清除代謝廢物并補充新鮮培養(yǎng)基;凍存則是為了長期保存細(xì)胞,而復(fù)蘇則是重新激活冷凍保存的細(xì)胞使其恢復(fù)正常生長。
換液周期:每周2-3次
NCI-H2286 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:4傳代;每周換液2次。;生長特性:貼壁生長;形態(tài)特性:橢圓形;相關(guān)產(chǎn)品有:SBC-5細(xì)胞、OVCAR.4細(xì)胞、NTera2/D1細(xì)胞
Hs-281-T Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代,每周換液2-3次。;生長特性:貼壁生長;形態(tài)特性:成纖維細(xì)胞;相關(guān)產(chǎn)品有:NPC-TW01細(xì)胞、OVCA432_Bast細(xì)胞、HT 1080細(xì)胞
NCI H747 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2—1:4傳代,每周換液2次;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:NKL細(xì)胞、HeLa-S3細(xì)胞、Colon 26細(xì)胞
背景信息:該細(xì)胞是1964年由FoghJ用移植培養(yǎng)方法和含15%FBS的F12培養(yǎng)從原發(fā)性腫瘤分離的。近來,已建株的培養(yǎng)細(xì)胞用含血清的McCoy's5a培養(yǎng)基培養(yǎng)。該細(xì)胞系在裸鼠中成瘤,也能在類固醇處理的地鼠中成瘤。該細(xì)胞可合成IgA、A、TGFβ結(jié)合蛋白和黏素;表達尿激酶受體,但沒有檢測到血漿酶原活性;不表達CD4,但細(xì)胞表面表達半乳糖神經(jīng)酰胺(HIV的可能替代受體)。該細(xì)胞系癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos陽性;p53基因過表達,并且在273位密碼子處發(fā)
HT-29人結(jié)腸癌細(xì)胞全年復(fù)蘇|已有STR圖譜
產(chǎn)品包裝:復(fù)蘇發(fā)貨:T25培養(yǎng)瓶(一瓶)或凍存發(fā)貨:1ml凍存管(兩支)
DSMZ菌株保藏中心成立于1969年,是德國的國家菌種保藏中心。該中心一直致力于細(xì)菌、真菌、質(zhì)粒、抗菌素、人體和動物細(xì)胞、植物病毒等的分類、鑒定和保藏工作。DSMZ菌種保藏中心是歐洲規(guī)模最大的生物資源中心,保藏有動物細(xì)胞500多株。Riken BRC成立于1920年,是英國的國家菌種保藏中心。該中心一直致力于細(xì)菌、真菌、植物病毒等的分類、鑒定和保藏工作。日本Riken BRC(Riken生物資源保藏中心)是全球三大典型培養(yǎng)物收集中心之一。Riken保藏中心提供了很多細(xì)胞系。在世界范圍內(nèi),這些細(xì)胞系,都在醫(yī)學(xué)、科學(xué)和獸醫(yī)中具有重要意義。Riken生物資源中心支持了各種學(xué)術(shù)、健康、食品和獸醫(yī)機構(gòu)的研究工作,并在世界各地不同組織的微生物實驗室和研究機構(gòu)中使用。
NCI H295R Cells;背景說明:腎上腺皮質(zhì)癌;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:NCI-H1092細(xì)胞、RERFLCMS細(xì)胞、SUIT2細(xì)胞
NIH:OVCAR-10 Cells;背景說明:卵巢癌;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:RPTEC TERT1細(xì)胞、HONE-1細(xì)胞、MDA MB 175 VII細(xì)胞
ROS17/28 Cells;背景說明:骨肉瘤;ACI 9935;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:Immortalized Human Hepatocytes細(xì)胞、MC57G細(xì)胞、IGROV-1細(xì)胞
4D3 [Mouse hybridoma against human unknown protein] Cells(提供STR鑒定圖譜)
來源說明:細(xì)胞主要來源ATCC、ECACC、DSMZ、RIKEN等細(xì)胞庫
物種來源:人源、鼠源等其它物種來源
HT-29人結(jié)腸癌細(xì)胞全年復(fù)蘇|已有STR圖譜
形態(tài)特性:上皮細(xì)胞樣
貼壁細(xì)胞消化傳代時通常采用兩種方法:一、加入胰酶等細(xì)胞脫落后,再加培養(yǎng)基中止胰酶作用,離心傳代;二、加入胰酶后,鏡下觀察待細(xì)胞始脫落時,棄胰酶,加培養(yǎng)分瓶。但前者太麻煩,而后者有可能對細(xì)胞施加胰酶選擇,因為總是貼壁不牢的細(xì)胞先脫落,對腫瘤細(xì)胞來說,這部分細(xì)胞有可能是惡性程度較GAO的細(xì)胞亞群。一種簡單的消化傳代方法。加入PBS洗去血清或加入胰酶先中和血清的作用(30s),棄之,再加入適量胰酶作用10s-40s(根據(jù)細(xì)胞消化的難易程度),棄之,這樣依賴殘余的胰酶就可將細(xì)胞消化單細(xì)胞。對于較難消化的細(xì)胞,可以用2%利多卡因消化5-8分鐘,然后再棄去,加培養(yǎng)基吹打也可以,對細(xì)胞的影響不大。不用PBS也不用Hanks洗,只要把舊培養(yǎng)吸的干凈一點,直接加酶消化應(yīng)該不會有什么問題。棄培養(yǎng)后,用0.04%的EDA沖洗一次,再用1/4v的0.04%的EDA室溫孵育5min,棄取大部分EDA,加入與剩余EDA等量的胰酶(預(yù)熱)總體積1/10v。消化到有細(xì)胞脫落。不過有人說EDA對細(xì)胞不HAO,有證據(jù)嗎?培養(yǎng)的BASMC:倒掉舊培養(yǎng)加入少量胰酶沖一下,倒掉再加入0.125-0.25%胰酶約6-10滴或1ml(25ml bole)消化再加入適量新培養(yǎng)基中和,并分瓶這種方法簡單、省事;效果很HAO并且不損失細(xì)胞!
Caco2-BBE Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:6—1:10傳代,每周換液2次;生長特性:貼壁生長;形態(tài)特性:上皮細(xì)胞;相關(guān)產(chǎn)品有:Hs729細(xì)胞、OCI-AML5細(xì)胞、Embryonic Bovine Tracheal cells細(xì)胞
COLO320-DM Cells;背景說明:該細(xì)胞可產(chǎn)生5-羥色胺、去甲、、ACTH和甲狀旁腺素。角蛋白、波形蛋白弱陽性。培養(yǎng)條件: RPMI 1640 10%FBS;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:懸浮+貼壁;形態(tài)特性:淋巴細(xì)胞;相關(guān)產(chǎn)品有:HuO9細(xì)胞、RBMEC細(xì)胞、Cloudman S91 melanoma clone M-3細(xì)胞
RenCa Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮細(xì)胞樣;相關(guān)產(chǎn)品有:NIE 115細(xì)胞、SVOG細(xì)胞、MT-2細(xì)胞
NCI-H3255 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長 ;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:CATH-a細(xì)胞、C8166-CD4細(xì)胞、H-196細(xì)胞
PLC PRF 5 Cells;背景說明:該細(xì)胞系分泌乙肝病毒表面抗原(HBsAg)。 此細(xì)胞系原先被支原體污染,后用BM-cycline去除支原體;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:RLE-6TN細(xì)胞、U-2932細(xì)胞、DMS-53細(xì)胞
Tu-212 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長 ;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:SNU-475細(xì)胞、NCI-H526細(xì)胞、V79-4細(xì)胞
K 562 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:Walker/LLC-WRC 256細(xì)胞、RPMI2650細(xì)胞、H-1568細(xì)胞
NCI-SNU-5 Cells;背景說明:該細(xì)胞來源于一名低分化胃癌患者的轉(zhuǎn)移性腹水,1987年分離建立。該細(xì)胞表達CEA和TAG-72。;傳代方法:2-3天補液一次。;生長特性:多細(xì)胞聚集、懸浮生長;形態(tài)特性:上皮細(xì)胞樣;相關(guān)產(chǎn)品有:HRMC細(xì)胞、RIN-14B細(xì)胞、THC-8307細(xì)胞
Mc Ardle 7777 Cells;背景說明:肝癌;雌性;Buffalo;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:PLC-PRF-5細(xì)胞、MBMEC細(xì)胞、SUDHL-6細(xì)胞
Hi5 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:JC細(xì)胞、SKCO1細(xì)胞、CEMx721.174.T2細(xì)胞
PC-10 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:NCI-HUT-69細(xì)胞、NCIH2591細(xì)胞、P3X63NS1細(xì)胞
LNCaP-Clone-FGC Cells;背景說明:人前列腺癌細(xì)胞LNCaP克隆FGC是從一位50歲白人男性(血型B+)的左鎖骨淋巴結(jié)針刺活檢中分離,該患者經(jīng)確診為前列腺癌轉(zhuǎn)移。 這株細(xì)胞對5-α-二睪酮(生長調(diào)節(jié)子和酸性脂酶產(chǎn)物)有響應(yīng)。這株細(xì)胞并不形成一致的單層,而是形成集落,在傳代時可以用滴管反復(fù)吹吸打碎。它們僅僅輕輕地吸附在基底上,不形成匯合,很快使培養(yǎng)基變酸。生長很慢。傳代后48小時內(nèi)不應(yīng)擾動。當(dāng)培養(yǎng)瓶封包后,多數(shù)細(xì)胞從培養(yǎng)瓶底分離,懸浮在培養(yǎng)基中。收到后,在通常培養(yǎng)單層細(xì)胞的條件下培養(yǎng)24到48小時,以合細(xì)胞再貼壁。;傳代方法:消化3-5分鐘。1:2。3天內(nèi)可長滿。;生長特性:貼壁生長;形態(tài)特性:上皮細(xì)胞;相關(guān)產(chǎn)品有:H1954細(xì)胞、3T6 Swiss Albino細(xì)胞、Tj-905細(xì)胞
Hep 3B Cells;背景說明:肝癌;男性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:Factor Dependent Continuous-Paterson 1細(xì)胞、SNGM細(xì)胞、HEK 293 c18細(xì)胞
E0771 Cells;背景說明:惡性乳腺癌;雌性;C57BL/6;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:Human Corneal Epithelial cells-Transformed細(xì)胞、BC-3-H-I細(xì)胞、kms 11細(xì)胞
BMF Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:4傳代;生長特性:貼壁生長;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:TK10細(xì)胞、SW 1990細(xì)胞、N9細(xì)胞
SUNE 1 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:Buffalo Rat Liver-3A細(xì)胞、SUDHL6細(xì)胞、GLAG-66細(xì)胞
LC1 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:PAa細(xì)胞、EA. hy 926細(xì)胞、AN3 CA細(xì)胞
Abcam HCT 116 BRD3 KO Cells(提供STR鑒定圖譜)
AG04457 Cells(提供STR鑒定圖譜)
BayGenomics ES cell line CSI167 Cells(提供STR鑒定圖譜)
BayGenomics ES cell line RST389 Cells(提供STR鑒定圖譜)
BFTC-905-diffuse M Cells(提供STR鑒定圖譜)
CHO203PV Cells(提供STR鑒定圖譜)
DA03036 Cells(提供STR鑒定圖譜)
DDaF-1 Cells(提供STR鑒定圖譜)
GM02527 Cells(提供STR鑒定圖譜)
WC00079 Cells;背景說明:黑色素瘤;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:A549ATCC細(xì)胞、MKN74細(xì)胞、D 407細(xì)胞
CLONE M3 Cells;背景說明:黑色素瘤;雄性;DBA;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:P3-X63-Ag 8.653細(xì)胞、A3細(xì)胞、HPAF-II細(xì)胞
Moorfields/Institute of Ophthalmology-Muller 1 Cells;背景說明:視網(wǎng)膜Muller細(xì)胞;自發(fā)永生;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:PC 12細(xì)胞、NCI-H810細(xì)胞、Hs 578.T細(xì)胞
H-820 Cells;背景說明:乳頭狀肺腺癌;淋巴結(jié)轉(zhuǎn)移;男性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:PC-3M IE8細(xì)胞、HNE-2細(xì)胞、Onda 11細(xì)胞
A-375 Cells;背景說明:A375源自一位54歲女性,是Giard DJ等人建立的一系列細(xì)胞株中的一株。該細(xì)胞可在免疫抑制小鼠上成瘤,在瓊脂上形成克隆。;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:上皮細(xì)胞樣;相關(guān)產(chǎn)品有:H647ell細(xì)胞、A.704細(xì)胞、Hs683T細(xì)胞
RPMI-1846 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:Hs940.T細(xì)胞、NCI-HUT-125細(xì)胞、HUASMC細(xì)胞
KLM-1 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:RPE1細(xì)胞、KALS1細(xì)胞、MEL細(xì)胞
PL-9 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:IOSE-29細(xì)胞、NCIH508細(xì)胞、NPC-039細(xì)胞
HT-29人結(jié)腸癌細(xì)胞全年復(fù)蘇|已有STR圖譜
MEL Cells;背景說明:DMSO可誘導(dǎo)該細(xì)胞向紅系分化。;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:上皮細(xì)胞樣;相關(guān)產(chǎn)品有:RS411細(xì)胞、QGP1細(xì)胞、TE 85 ClF-5細(xì)胞
He La Cells;背景說明:HeLa是第一個來自人體組織經(jīng)連續(xù)培養(yǎng)獲得的非整倍體上皮樣細(xì)胞系,它由GeyGO等在1951年從31歲女性黑人的宮頸癌組織建立。經(jīng)原始組織切片重新觀察,Jones等將其診斷為腺癌。已知該細(xì)胞系含有人乳頭狀瘤病毒HPV18序列,需在2級生物安全防護臺操作。該細(xì)胞角蛋白陽性,p53表達量較低,但表達正常水平的pRB(視網(wǎng)膜母細(xì)胞瘤抑制因子)。;傳代方法:1:3傳代,2-3天換液一次;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:C81-61細(xì)胞、EST50細(xì)胞、M210B4細(xì)胞
GA-10 clone 4 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:每2-3天換液;生長特性:懸浮生長 ;形態(tài)特性:淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有:P388.D1細(xì)胞、Hs 636 T細(xì)胞、SNU-869細(xì)胞
Caki-1 Cells;背景說明:該細(xì)胞超微結(jié)構(gòu)中包含許多微絨毛、少許微絲、許多小線粒體、發(fā)達的高爾基休和內(nèi)質(zhì)網(wǎng)、許多脂滴和多層體、次級溶酶體,沒有發(fā)現(xiàn)病毒顆粒。;傳代方法:1:2-1:4傳代;每周換液2-3次。;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:NPA-87細(xì)胞、NCIH929細(xì)胞、GM-3573細(xì)胞
A375S2 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:H1341細(xì)胞、PANC 1005細(xì)胞、PNT1-a細(xì)胞
A375S2 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:H1341細(xì)胞、PANC 1005細(xì)胞、PNT1-a細(xì)胞
Reuber H-35 Cells;背景說明:在糖皮質(zhì)激素、胰島素或cAMP衍生物的誘導(dǎo)下可以產(chǎn)生酪酸基轉(zhuǎn)移酶;可被逆轉(zhuǎn)錄病毒感染;可產(chǎn)生白蛋白、轉(zhuǎn)鐵蛋白、凝血酶原;在AxC大鼠中可以成瘤。;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:STO細(xì)胞、Hs940-T細(xì)胞、PL 5細(xì)胞
GM12601 Cells(提供STR鑒定圖譜)
HAP1 EPT1 (-) 1 Cells(提供STR鑒定圖譜)
H-1436 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:隨細(xì)胞的密度而增加;生長特性:懸浮生長;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:PNT1/A細(xì)胞、SUM-52-PE細(xì)胞、Hca-F細(xì)胞
TALL-104 Cells;背景說明:急性T淋巴細(xì)胞白血??;男性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:懸浮;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:Mino細(xì)胞、LTEP-sm 1細(xì)胞、HEK-293-EBNA細(xì)胞
Monomac-1 Cells;背景說明:急性單核細(xì)胞白血?。荒行?傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:懸浮;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:H2228細(xì)胞、Panc 10.05細(xì)胞、SUM190細(xì)胞
HT 1080.T Cells;背景說明:該細(xì)胞源自一名35歲患有纖維肉瘤的白人男性的結(jié)締組織;ras+。;傳代方法:1:4-1:8傳代;2-3天換液1次。;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:SK-Hep1細(xì)胞、HANK細(xì)胞、HFL細(xì)胞
HeLa/S3 Cells;背景說明:該細(xì)胞是1955年由PuckTT,MarcusPI和CieciuraSJ建系的,含HPV-18序列;角蛋白陽性;可用于與染色體突變、細(xì)胞營養(yǎng)、集落形成相關(guān)的哺乳動物細(xì)胞的克隆分析。;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:Lilly Laboratories Cell-Porcine Kidney 1細(xì)胞、NCTC1469細(xì)胞、GP-293細(xì)胞
JJN3 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:DrG細(xì)胞、SK-RC 39細(xì)胞、MiaPaca.2細(xì)胞
SKES-1 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:5傳代;每周換液2-3次;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:EFM-192B細(xì)胞、WRL 68細(xì)胞、KMB-17細(xì)胞
Wistar Institute-38 Cells;背景說明:LeonardHayflick建系;有限傳代細(xì)胞系;壽命為50±10代(倍增時間24h);來自妊娠3個月的正常胚胎肺組織。該細(xì)胞系是第一個用于人制備的人二倍體細(xì)胞;培養(yǎng)基中添加TNFα可以加快細(xì)胞生長。;傳代方法:1:2-1:4傳代;2-3天換液1次;生長特性:貼壁生長;形態(tài)特性:成纖維細(xì)胞樣;相關(guān)產(chǎn)品有:PK(15)細(xì)胞、WM115細(xì)胞、PC 61-5-3細(xì)胞
HiPS-RIKEN-2C Cells(提供STR鑒定圖譜)
iPS NIHi2 Cells(提供STR鑒定圖譜)
MB352 Cells(提供STR鑒定圖譜)
ND10565 Cells(提供STR鑒定圖譜)
PL14 [Human pancreatic adenocarcinoma] Cells(提供STR鑒定圖譜)
Ubigene HeLa TRAF5 KO Cells(提供STR鑒定圖譜)
XPH18PV Cells(提供STR鑒定圖譜)
HG02696 Cells(提供STR鑒定圖譜)
INS1 Cells;背景說明:該細(xì)胞源自X射線照射的移植胰島瘤的大鼠,胰島素陽性,可合成胰島素原I和II,可用于beta細(xì)胞功能研究。;傳代方法:1:3—1:6傳代,每周換液2—3次;生長特性:貼壁生長;形態(tài)特性:不規(guī)則,多角;相關(guān)產(chǎn)品有:OVCAR-8細(xì)胞、L5178Y TK+/- (clone 3.7.2C)細(xì)胞、MRCV細(xì)胞
NEC Cells;背景說明:食管癌;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:U14細(xì)胞、H1355細(xì)胞、Clone M-3細(xì)胞
WB-F344 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:NCI-H211細(xì)胞、Kit225/K6細(xì)胞、WM239A細(xì)胞
OVCA-432 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:MRC-5細(xì)胞、NCI-H209細(xì)胞、SCC90細(xì)胞
Panc10.05 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:MUGCHOR1細(xì)胞、MLE-12細(xì)胞、Japanese Tissue Culture-28細(xì)胞
NCIH524 Cells;背景說明:該細(xì)胞1982年建系,源自非小細(xì)胞肺癌男性患者的轉(zhuǎn)移淋巴結(jié)。;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:懸浮生長;形態(tài)特性:圓形細(xì)胞;相關(guān)產(chǎn)品有:SDBMSC細(xì)胞、MDA-415細(xì)胞、Kit225細(xì)胞
Ca-Ski Cells;背景說明:這株細(xì)胞是從小腸腸系膜轉(zhuǎn)移灶的細(xì)胞中建立的。 據(jù)報道,它含有完整的HPV-16(每個細(xì)胞大約600個拷貝)和HPV-18相關(guān)序列。;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮細(xì)胞樣;相關(guān)產(chǎn)品有:LIM1215細(xì)胞、KPNRTBM1細(xì)胞、L-1210細(xì)胞
Jiyoye Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:每周2-3次。;生長特性:懸浮生長;形態(tài)特性:淋巴母細(xì)胞;相關(guān)產(chǎn)品有:ABC-1細(xì)胞、LNCaP C4-2細(xì)胞、3AO細(xì)胞
COR-L279 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:NS1細(xì)胞、Caov-4細(xì)胞、HCC0015細(xì)胞
TE-15 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:消化3-5分鐘。1:2。3天內(nèi)可長滿。;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:MV4:11細(xì)胞、CHOS細(xì)胞、NCI-H820細(xì)胞
NCI-H69C Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2—1:4傳代,每周換液2次;生長特性:懸浮生長,聚團;形態(tài)特性:聚團懸浮;相關(guān)產(chǎn)品有:SK.MEL.28細(xì)胞、KNS42細(xì)胞、HEK;293細(xì)胞
NCI-H810 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:6傳代,每周2-3次。;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:ACHN細(xì)胞、JB6 Cl 30-7b細(xì)胞、H2228細(xì)胞
GM2219C Cells;背景說明:MOLT-4與MOLT-3來源于一名19歲的男性急性淋巴細(xì)胞性白血病的復(fù)發(fā)患者,該患者前期接受過多種藥物聯(lián)合化療。MOLT-4細(xì)胞系為T淋巴細(xì)胞起源,p53基因的第248位密碼子有一個G→A突變,不表達p53,不表達免疫球蛋白或EB病毒;可產(chǎn)生高水平的末端脫氧核糖轉(zhuǎn)移酶;表達CD1(49%),CD2(35%),CD3A(26%)B(33%)C(34%),CD4(55%),CD5(72%),CD6(22%),CD7(77%)。;傳代方法:1:2傳代;生長特性:懸浮生長;形態(tài)特性:淋巴母細(xì)胞樣;圓形;相關(guān)產(chǎn)品有:AK細(xì)胞、HEK 293-EBNA細(xì)胞、Loucy細(xì)胞
CCD-841CoN Cells;背景說明:結(jié)腸上皮細(xì)胞;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:HPF細(xì)胞、Tsup-1細(xì)胞、hTERT-RPE細(xì)胞
HKBML Cells;背景說明:腦;淋巴瘤;男性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:懸浮;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:HL-1細(xì)胞、8226細(xì)胞、SW 962細(xì)胞
SG5-1.13 Cells(提供STR鑒定圖譜)
PC9 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:CNE1細(xì)胞、HME-1細(xì)胞、SU-DHL-6細(xì)胞
ID8/MOSEC Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代;生長特性:貼壁生長 ;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:QGP1細(xì)胞、NCIN87細(xì)胞、ADR-RES細(xì)胞
OVCAR 420 Cells;背景說明:卵巢癌;女性;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:Panc 02.03細(xì)胞、GM01232細(xì)胞、Hs675T細(xì)胞
NCIADRRES Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:RWPE-1細(xì)胞、CEL細(xì)胞、Mesothelial cells transfected with pRSV-T 5A細(xì)胞
PA-1 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:4-1:10傳代;每周2-3次。 ;生長特性:貼壁生長;形態(tài)特性:上皮細(xì)胞;相關(guān)產(chǎn)品有:Becker細(xì)胞、NUGC-2細(xì)胞、NCIH1819細(xì)胞
Hep G2 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:SKLU1細(xì)胞、NCI-H187細(xì)胞、C8166-CD4細(xì)胞
HCC-9204 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:MNNGHOS細(xì)胞、Sp2/0-Ag-14細(xì)胞、FTC-133細(xì)胞
MD Anderson-Metastatic Breast-435 Cells;背景說明:乳腺癌;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:LNCaP-FGC細(xì)胞、HOC-1細(xì)胞、CMT-64細(xì)胞
HT-29人結(jié)腸癌細(xì)胞全年復(fù)蘇|已有STR圖譜
Hs 895 T Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:4傳代,2-3天換液1次。;生長特性:貼壁生長;形態(tài)特性:成纖維細(xì)胞;相關(guān)產(chǎn)品有:CP-70細(xì)胞、THP-1細(xì)胞、MLTC-1細(xì)胞
Hs 281.T Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2傳代,每周換液2-3次。;生長特性:貼壁生長;形態(tài)特性:成纖維細(xì)胞;相關(guān)產(chǎn)品有:COLO-320-DM細(xì)胞、PNT1-a細(xì)胞、Roswell Park Memorial Institute 7951細(xì)胞
H-522 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:3-1:6傳代;每周換液2-3次。;生長特性:貼壁生長;形態(tài)特性:上皮樣;相關(guān)產(chǎn)品有:HEK-293-F細(xì)胞、Hs 840.T細(xì)胞、RWPE-1細(xì)胞
JHH-7 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:GM03671細(xì)胞、NCI-H1954細(xì)胞、RMS1598細(xì)胞
H-735 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:2-1:3傳代;每周換液2-3次。;生長特性:貼壁或懸浮,詳見產(chǎn)品說明書部分;形態(tài)特性:詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有:SKNBE(1)細(xì)胞、GM07404細(xì)胞、HREC細(xì)胞
HCC-1954 Cells;背景說明:詳見相關(guān)文獻介紹;傳代方法:1:4-1:8傳代;每周換液2-3次。;生長特性:偶爾上皮細(xì)胞空泡;形態(tài)特性:上皮細(xì)胞樣;相關(guān)產(chǎn)品有:EM3細(xì)胞、HCC9724細(xì)胞、H-1568細(xì)胞
BayGenomics ES cell line DTM026 Cells(提供STR鑒定圖譜)
BayGenomics ES cell line TEA205 Cells(提供STR鑒定圖譜)
CCRC-M78 Cells(提供STR鑒定圖譜)
MA-Balb Cells(提供STR鑒定圖譜)
Royan B2 Cells(提供STR鑒定圖譜)
T3-2 [Rat tooth germ] Cells(提供STR鑒定圖譜)
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Pathol. Oncol. R"
關(guān)鍵字: HT-29人結(jié)腸癌細(xì)胞全年復(fù)蘇|已有ST;傳代細(xì)胞;復(fù)蘇細(xì)胞;實驗細(xì)胞;科研細(xì)胞;
上海冠導(dǎo)生物工程有限公司,先后從ATCC、DSMZ、ECACC、RIKEN、PromoCell、ScienCell、JCRB等國內(nèi)外細(xì)胞庫引進細(xì)胞2000余株。以此為契機,公司組建了冠導(dǎo)細(xì)胞庫,我司細(xì)胞均由資深細(xì)胞培養(yǎng)工程師進行培養(yǎng)。我司可以提供的細(xì)胞有:①細(xì)胞系②原代細(xì)胞③穩(wěn)轉(zhuǎn)株④耐藥株⑤標(biāo)記細(xì)胞⑥細(xì)胞配套試劑等。