"BT-474[BT474]人乳腺導(dǎo)管癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

傳代比例：1:2-1:4(首次傳代建議1:2)

生長特性：貼壁生長

【細(xì)胞培養(yǎng)經(jīng)驗(yàn)分享】啟蒙老師的重要性：一般進(jìn)實(shí)驗(yàn)室都有師兄師姐帶著做，他們就是你做細(xì)胞的啟蒙老師。他們的操作手法、細(xì)節(jié)、理論講解就成了你操作的準(zhǔn)則，如營養(yǎng)液、細(xì)胞瓶的擺放位置、滅菌處理程序、開蓋手法、細(xì)胞吹打手法等等。要學(xué)會(huì)他們的正確操作，在第一次的時(shí)候就要重視。像養(yǎng)孩子一樣養(yǎng)細(xì)胞，細(xì)胞有時(shí)真的很脆弱，最好每天都去看看它，以防止出現(xiàn)培養(yǎng)箱缺水、缺二氧化碳、停電、溫度不夠等異?，F(xiàn)象，也好及時(shí)解決這些意外，避免重復(fù)實(shí)驗(yàn)帶來的更大痛苦。好細(xì)胞要及時(shí)保種：細(xì)胞要分批傳代，這樣即使有一批出了問題，還有一批備用的。像后者一般人可能不容易做到。但這是我血的教訓(xùn)，有一次細(xì)胞污染了，全軍覆沒。當(dāng)時(shí)可后悔沒有保種。細(xì)胞跟人一樣，不同的細(xì)胞，培養(yǎng)特性是不一樣的。培養(yǎng)過程中要細(xì)細(xì)體會(huì)，不同細(xì)胞系使用不同的培養(yǎng)基和血清。

換液周期：每周2-3次

OVCAR3 Cells;背景說明：該細(xì)胞１９８２年由T.C. Hamilton等建系，源自一位６０卵巢腺癌的腹水，是卵巢癌抗藥性研究的模型。;傳代方法：１：２—１：４傳代，每周換液２—３次;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：BLO-11細(xì)胞、SNB.19細(xì)胞、NCI-H711細(xì)胞

PLA801C Cells;背景說明：肺巨細(xì)胞癌;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MBT-2細(xì)胞、CCD-19Lu細(xì)胞、Experimental Mammary Tumour-6細(xì)胞

KMS-11 Cells;背景說明：多發(fā)性骨髓瘤;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NOR10細(xì)胞、NCIH2106細(xì)胞、BT細(xì)胞

BT-474[BT474]人乳腺導(dǎo)管癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

背景信息：是由E·Lasfargues和W·G·Coutinho從一個(gè)實(shí)心的乳腺浸潤性導(dǎo)管癌中分離到的細(xì)胞系。最初分離自一名60歲白人女性的乳腺浸潤性導(dǎo)管癌組織。

┈訂┈購(技術(shù)服務(wù))┈熱┈線：1┈3┈6┈4┈1┈9┈3┈0┈7┈9┈1【微信同號】┈Q┈Q：3┈1┈8┈0┈8┈0┈7┈3┈2┈4；

貼壁細(xì)胞的傳代培養(yǎng)，詳細(xì)步驟如下：首先倒掉培養(yǎng)基，在這一步驟可以收集一些細(xì)胞上清做支原體檢測；加入胰蛋白酶，一般T25是加2mL，蓋好瓶蓋，搖晃T25培養(yǎng)瓶，使胰蛋白酶均勻覆蓋在細(xì)胞表面，放入培養(yǎng)箱2-3min，期間可在顯微鏡下觀察，看到大部分細(xì)胞變圓，即可放入超凈臺(tái)，加入2倍的完全培養(yǎng)基，這里就是加4mL培養(yǎng)基，終止消化；將含有胰蛋白酶，細(xì)胞和培養(yǎng)基一起轉(zhuǎn)移到離心管中，1000rpm/3min離心，去掉上清；新鮮的完全培養(yǎng)基重懸，根據(jù)細(xì)胞的生長特性和后續(xù)的實(shí)驗(yàn)需求進(jìn)行傳代，比如我養(yǎng)的Hepa1-6就長的比較快，不是著急用的話，我就會(huì)按1E6個(gè)細(xì)胞/T75培養(yǎng)瓶進(jìn)行傳代；但如果后兩天要用，就會(huì)適當(dāng)多傳一點(diǎn)；還可通過顯微鏡計(jì)數(shù)后，直接用于細(xì)胞鋪板，繼續(xù)后續(xù)的實(shí)驗(yàn)。

產(chǎn)品包裝：復(fù)蘇發(fā)貨：T25培養(yǎng)瓶(一瓶)或凍存發(fā)貨：1ml凍存管(兩支)

來源說明：細(xì)胞主要來源ATCC、ECACC、DSMZ、RIKEN等細(xì)胞庫

BT-474[BT474]人乳腺導(dǎo)管癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

物種來源：人源、鼠源等其它物種來源

HS578 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CAMA-1細(xì)胞、SCCVII/St細(xì)胞、MT-3 [Human leukocytes]細(xì)胞

DLM8 Cells;背景說明：Dunn's骨肉瘤；雌性；C３H;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：TFH細(xì)胞、U-343細(xì)胞、CC-LP-1細(xì)胞

Clone 166 Cells;背景說明：血管內(nèi)皮;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CCD-33Co細(xì)胞、COLO 320F細(xì)胞、SV40 MES 13細(xì)胞

MCF-12A Cells;背景說明：非致瘤性乳腺上皮細(xì)胞；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：LSC-1細(xì)胞、SKHEP-1細(xì)胞、EB-2細(xì)胞

┈訂┈購(技術(shù)服務(wù))┈熱┈線：1┈3┈6┈4┈1┈9┈3┈0┈7┈9┈1【微信同號】┈Q┈Q：3┈1┈8┈0┈8┈0┈7┈3┈2┈4；

形態(tài)特性：上皮細(xì)胞樣

細(xì)胞復(fù)蘇后貼壁細(xì)胞較少的問題分析：總結(jié)１：復(fù)蘇過程沒有問題，是否是從拿出直接放入溫水，還有培養(yǎng)箱，二氧化碳濃度，培養(yǎng)基、PH值等環(huán)節(jié)。要么加GAO濃度FBS １５-２０%，看看能否幫助貼壁，當(dāng)然也需要考慮血清問題，還有確信拿來的細(xì)胞沒問題?？偨Y(jié)２：首先應(yīng)該懷疑凍存，實(shí)際上復(fù)蘇出問題的可能非常小，因?yàn)椴僮骱唵?，而且死板。１、你凍存的時(shí)候是不是消化的時(shí)間過長，這是一般人所注意不到的，即使書上也不講這個(gè)問題，太長的消化時(shí)間會(huì)讓細(xì)胞復(fù)蘇時(shí)失去貼壁能力，表現(xiàn)為先貼后死，原因是在你復(fù)蘇的時(shí)候細(xì)胞已進(jìn)入凋亡程序，不可逆轉(zhuǎn)的死亡。２、你的凍存HAO不HAO，是什么，甘油還是DMSO，質(zhì)量非常重要，否則也會(huì)死亡。３、你的凍存的量加的是不是太多，AC推薦是不超過７%，大于５%，太多也不HAO。４、你在凍存的時(shí)候是不是把DMSO混均勻，這個(gè)有一些影響，但不算太大。５、你的凍存是否按部就班，就是所溫度梯度是不是把握嚴(yán)格，很多人容易忘卻這個(gè)事情，因?yàn)檫@個(gè)東西流程長。６、如果你細(xì)胞污染，你是否能很快看到，我比我的導(dǎo)師能早一天看到污染。從這個(gè)角度講建議去除離心這步。７、你的細(xì)胞在凍存前是否過密。還有，不贊成孵箱污染這個(gè)概念的，所有在一個(gè)孵箱里的細(xì)胞都污染一個(gè)細(xì)菌的話，這個(gè)細(xì)菌是源于孵箱的，但這不代表孵箱污染，因?yàn)榉跸錈o論你如何處理都有大量的細(xì)菌，問題在操作。每次污染的原因都要盡可能的找，以后就不犯同樣的問題，這個(gè)很重要，不能靠猜，否則你就有可能細(xì)胞養(yǎng)絕Zui后換課題，這個(gè)見得太多了，別不當(dāng)會(huì)事。

Pa14C Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：DLD 1細(xì)胞、HL7702細(xì)胞、RPMI no. 8226細(xì)胞

BHT-101 Cells;背景說明：甲狀腺癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MSTO211H細(xì)胞、DV-90細(xì)胞、JeKo 1細(xì)胞

U-118-MG Cells;背景說明：注意： 據(jù)報(bào)道來自不同個(gè)體的膠質(zhì)母細(xì)胞瘤細(xì)胞株U-１１８ MG (HTB-１５) 和 U-１３８ MG (HTB-１６)有著一致的VNTR和相近的STR模式。 U-１１８ MG 和 U-１３８ MG細(xì)胞遺傳學(xué)上很相似并有至少六個(gè)衍生標(biāo)記染色體。 這是１９６６年至１９６９年間J. Ponten和同事從惡性神經(jīng)膠質(zhì)瘤中構(gòu)建的細(xì)胞株中的一株(其它包括ATCC HTB-１４和 ATCC HTB-１６ and ATCC HTB-１７)。 １９８７年用BM-Cycline培養(yǎng)６周去除了支原體污染。 ;傳代方法： 消化３-５分鐘。１：２傳代。３天內(nèi)可長滿。;生長特性：貼壁生長;形態(tài)特性：混合型;相關(guān)產(chǎn)品有：KP4細(xì)胞、Hs611T細(xì)胞、NB-9細(xì)胞

Hs 274.T Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代,每周換液２-３次。;生長特性：貼壁生長;形態(tài)特性：成纖維細(xì)胞;相關(guān)產(chǎn)品有：KU.812細(xì)胞、HECV細(xì)胞、NCIH23細(xì)胞

OCM1 Cells;背景說明：葡萄膜黑色素瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CTV-1細(xì)胞、hTERTHME1細(xì)胞、CL-11細(xì)胞

NCI-HUT-125 Cells;背景說明：腺鱗狀肺癌；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Ramos.2G6.4C10細(xì)胞、Jurkat E6細(xì)胞、C33細(xì)胞

CORL105 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HT-29細(xì)胞、Ontario Cancer Institute-Acute Myeloid Leukemia-3細(xì)胞、MDA-MB 361細(xì)胞

CCFSTTG1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３—１：６傳代；每周換液２-３次;生長特性：貼壁生長;形態(tài)特性：星形膠質(zhì)細(xì)胞;相關(guān)產(chǎn)品有：CORL105細(xì)胞、HSAEC1-KT細(xì)胞、HCC2279細(xì)胞

SUDHL-8 Cells;背景說明：彌漫大B淋巴瘤；腹腔積液轉(zhuǎn)移；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SK-MEL-1細(xì)胞、OCI-Ly18細(xì)胞、LS123細(xì)胞

Namalva Cells;背景說明：這株細(xì)胞有EB病毒基因組。;傳代方法：１：２傳代。３天內(nèi)可長滿。;生長特性：懸浮生長 ;形態(tài)特性：淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有：Nakata-1細(xì)胞、FF-WT-BJ細(xì)胞、Tca-83細(xì)胞

SUDHL2 Cells;背景說明：彌漫性大細(xì)胞淋巴瘤；胸腔積液轉(zhuǎn)移；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：L-M (TK-)細(xì)胞、SV-HUC細(xì)胞、NCTC929細(xì)胞

NCI-H446 Cells;背景說明：該細(xì)胞是１９８２年由CarneyD和GazdarAF等從一位小細(xì)胞肺癌患者的胸腔積液中建立的。細(xì)胞的原始形態(tài)并不具有小細(xì)胞肺癌特征。這個(gè)細(xì)胞株是小細(xì)胞肺癌的生化和形態(tài)學(xué)上的變種，表達(dá)神經(jīng)元特有的烯醇酶和腦型肌酸激酶同工酶；左旋多巴脫羧酶、蠶素、抗利尿激素、催產(chǎn)素或胃泌激素釋放肽未達(dá)到可檢測水平。與正常細(xì)胞相比，該細(xì)胞c-mycDNA序列擴(kuò)增約２０倍，RNA增加１５倍。最初傳代培養(yǎng)基用含有５%FBS的RPMI１６４０，另外添加１０nM化可的松、０.００５mg/ml胰島素、０.０１mg/ml轉(zhuǎn)鐵;傳代方法：１：２傳代;生長特性：貼壁/懸浮生長，混合;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：WM 451-Lu細(xì)胞、TALL1細(xì)胞、H-226細(xì)胞

CHL/IU Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCI-747細(xì)胞、DoTc2細(xì)胞、MOLP8細(xì)胞

NCI-H2126 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：４傳代，每周２-３次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：NCIH1651細(xì)胞、BNL-HCC細(xì)胞、H-441細(xì)胞

P3J.HR1K Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：每２-３天換液;生長特性：懸浮生長 ;形態(tài)特性：淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有：H-510A細(xì)胞、B16BL6細(xì)胞、SUSM1細(xì)胞

kms 11 Cells;背景說明：多發(fā)性骨髓瘤;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Hopkins-92細(xì)胞、293-EBNA1細(xì)胞、H-1155細(xì)胞

Human Lung Microvascular Endothelial Cell line-5a Cells;背景說明：肺微血管；內(nèi)皮細(xì)胞；SV４０轉(zhuǎn)化;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HS940細(xì)胞、S.B.細(xì)胞、Ca761細(xì)胞

2008/C13*5.25 Cells(提供STR鑒定圖譜)

Abcam HeLa NFATC1 KO Cells(提供STR鑒定圖譜)

AG24166 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line RRG360 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line XH056 Cells(提供STR鑒定圖譜)

C0920 Cells(提供STR鑒定圖譜)

Cx43KOM104-E4(+/-) Cells(提供STR鑒定圖譜)

DA04958 Cells(提供STR鑒定圖譜)

FY-hES-9 Cells(提供STR鑒定圖譜)

Vero C1008 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：COLO 206F細(xì)胞、SU 86.86細(xì)胞、TW01細(xì)胞

BT-474[BT474]人乳腺導(dǎo)管癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

H-1581 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：每周換液２次。;生長特性：混合型;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：NCI-H841細(xì)胞、HLE細(xì)胞、YH細(xì)胞

MC3T3, E1 subgroup-4 clone Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Ketr-3細(xì)胞、HAPI細(xì)胞、C-6細(xì)胞

2780CP Cells;背景說明：卵巢癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：KOPN8細(xì)胞、TFH細(xì)胞、CHP-100細(xì)胞

HRVEC Cells;背景說明：視網(wǎng)膜微血管；內(nèi)皮 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CCK-81細(xì)胞、COLO_320DM細(xì)胞、L-6細(xì)胞

TCC Sup Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：Hs 695.T細(xì)胞、OCI-Ly01細(xì)胞、OCILY10細(xì)胞

145-2C11 Cells(提供STR鑒定圖譜)

Walker 256 Cells;背景說明：乳腺癌；雌性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Centre Antoine Lacassagne-85-1細(xì)胞、GBC-SD細(xì)胞、BHP 10-3細(xì)胞

Panc4.03 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：RMG-1細(xì)胞、EB2細(xì)胞、Melanoma 14細(xì)胞

SK-Hep1 Cells;背景說明：SK-HEP-１細(xì)胞系已被鑒定為內(nèi)皮來源。該細(xì)胞系為異倍體女性人（XX），染色體在亞三倍體范圍內(nèi)。在裸鼠中，它能形成與肝癌相一致的大細(xì)胞癌;傳代方法：１：３傳代，２-３天換液一次;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Hepatoma 22細(xì)胞、YH細(xì)胞、Mink Lung細(xì)胞

H1573 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代，每周２-３次。;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HUT 125細(xì)胞、OSK-1細(xì)胞、NK-10A細(xì)胞

FLC-7 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：B16 F10細(xì)胞、B95-8細(xì)胞、Menschliche Und Tierische Zellkulture-1細(xì)胞

KNS-81 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：H-1876細(xì)胞、RS1細(xì)胞、BRL 3A細(xì)胞

RCC 786-O Cells;背景說明：該細(xì)胞源自一位原發(fā)性腎透明細(xì)胞癌患者。該細(xì)胞有微絨毛和橋粒，能在軟瓊脂上生長。此細(xì)胞生成一種PTH（甲狀旁腺素）樣的多肽，與乳癌和肺癌中生成的肽相似，其N端序列與PTH相似，具有PTH樣活性，分子量為６０００D。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：IM95細(xì)胞、NCI-H1869細(xì)胞、T-84細(xì)胞

NCI-H1770 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：隨細(xì)胞的生長而換液;生長特性：懸浮生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SKGIIIA細(xì)胞、HuH28細(xì)胞、TK.10細(xì)胞

GM28232 Cells(提供STR鑒定圖譜)

HAP1 PCGF2 (-) Cells(提供STR鑒定圖譜)

OV2008 Cells;背景說明：宮頸鱗癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NS1-Ag4/1細(xì)胞、HRC-99細(xì)胞、NCI-H727細(xì)胞

SK-GT-4 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：6-10B細(xì)胞、H1963細(xì)胞、M2-10B4細(xì)胞

KNS-62 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：每周換液２次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：UMNSAH-DF-1細(xì)胞、SK-N-BE(2)細(xì)胞、MNNG/HOS細(xì)胞

RMa-bm Cells;背景說明：骨髓巨噬 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：半懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CT26WT細(xì)胞、HEL-92.1.7細(xì)胞、HCC2218細(xì)胞

Tu212 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：FOX-NY細(xì)胞、RT4P細(xì)胞、H-1436細(xì)胞

H-1299 Cells;背景說明：這株細(xì)胞來源于一個(gè)淋巴結(jié)轉(zhuǎn)移?；颊呓邮芰顺跗诜暖?。細(xì)胞均一性的部分缺失p５３蛋白，并缺少p５３蛋白表達(dá)。細(xì)胞可以合成０.１pmol/毫克蛋白的NMB蛋白，而不合成促胃液釋放肽(GRP)。;傳代方法：１：２傳代；３天傳代一次。;生長特性：貼壁生長;形態(tài)特性：上皮樣；多角形;相關(guān)產(chǎn)品有：T98G細(xì)胞、P116細(xì)胞、MFE 296細(xì)胞

NMCG-1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：GM00215細(xì)胞、TE-32細(xì)胞、CAL 62細(xì)胞

NGEC Cells;背景說明：胃黏膜；上皮 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Duke University 145細(xì)胞、TCam-2細(xì)胞、SF-763細(xì)胞

HS362 Cells(提供STR鑒定圖譜)

KOLF2.1J LRRK2 R1441H SNV/SNV Cells(提供STR鑒定圖譜)

MNZTASi011-A Cells(提供STR鑒定圖譜)

NUIGi053-A Cells(提供STR鑒定圖譜)

RG-241 Cells(提供STR鑒定圖譜)

U-1231MG Cells(提供STR鑒定圖譜)

UM-SCV-1A Cells(提供STR鑒定圖譜)

HG01517 Cells(提供STR鑒定圖譜)

HGMC Cells;背景說明：腎小球系膜;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MFE 296細(xì)胞、DSL-6A/C1細(xì)胞、OVCAR-8細(xì)胞

SKBR-3 Cells;背景說明：這株細(xì)胞源自胸水。沒有病毒顆粒。亞顯微結(jié)構(gòu)特征包括微絲和橋粒，肝糖原顆粒，大溶酶體，成束的細(xì)胞質(zhì)纖絲。SK-BR-３細(xì)胞株過表達(dá)HER２/c-erb-２基因產(chǎn)物。;傳代方法：消化３-５分鐘，１：２,３天內(nèi)可長滿;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：RPMI 1846細(xì)胞、NCIH2085細(xì)胞、RT-BM-1細(xì)胞

SY5Y Cells;背景說明：據(jù)報(bào)道，該細(xì)胞的密度可高達(dá)１×１０６cells/cm２，具有中等水平的多巴胺β羥化酶的活性。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：DBTRG-05MG細(xì)胞、BHT-101細(xì)胞、TE11細(xì)胞

SK-UT-1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：４-１：１２傳代，２天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：TE-14細(xì)胞、3-LL細(xì)胞、HPAEpiC細(xì)胞

Hs 839.T Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：成纖維細(xì)胞;相關(guān)產(chǎn)品有：NCI-H661細(xì)胞、HEK 293細(xì)胞、HEK293-A細(xì)胞

Hs 839.T Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：成纖維細(xì)胞;相關(guān)產(chǎn)品有：NCI-H661細(xì)胞、HEK 293細(xì)胞、HEK293-A細(xì)胞

OSRC2 Cells;背景說明：來源于日本人的腎臟腫瘤細(xì)胞。 可以移植到裸鼠。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：GL261細(xì)胞、Hep 3B2.1-7細(xì)胞、CA-OV-3細(xì)胞

L23/P Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：ETCC007細(xì)胞、Mink細(xì)胞、Primary Liver Carcinoma/Poliomyelitis Research Foundation/5細(xì)胞

MAC1 Cells;背景說明：皮膚T淋巴瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MuM-2B細(xì)胞、HCC-1428細(xì)胞、SACC-LM細(xì)胞

Fu97 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：KRC/Y細(xì)胞、T 84細(xì)胞、LN 229細(xì)胞

Hs 636 T Cells;背景說明：宮頸鱗癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SNG-M細(xì)胞、Hs.27細(xì)胞、A-101D細(xì)胞

Hs 870.T Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２—１：３傳代；每周換液２-３次;生長特性：貼壁生長;形態(tài)特性：成纖維細(xì)胞;相關(guān)產(chǎn)品有：RPVSMC細(xì)胞、MRC5細(xì)胞、KYSE510細(xì)胞

NCIH1355 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：每周換液２次。;生長特性：懸浮生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HMSC細(xì)胞、D341細(xì)胞、T. T細(xì)胞

GA10 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：每周２-３次;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞;相關(guān)產(chǎn)品有：H-322細(xì)胞、HCT116/L細(xì)胞、TK+/- (clone 3.7.2C)細(xì)胞

LK-2 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：RAEC細(xì)胞、COLO 829細(xì)胞、A1847細(xì)胞

STBCi322-A-1 Cells(提供STR鑒定圖譜)

VMM-5A Cells;背景說明：黑色素瘤；神經(jīng)節(jié)轉(zhuǎn)移；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CC-LP-I細(xì)胞、AtT20細(xì)胞、PL-9細(xì)胞

Porcine Kidney-13 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２—１：４傳代，每周換液２—３次;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：H-1048細(xì)胞、P3HRI細(xì)胞、HPAF II細(xì)胞

BHP10-3 Cells;背景說明：甲狀腺乳頭狀癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：A375-SM細(xì)胞、MES23.5細(xì)胞、SKGT2細(xì)胞

S3-HeLa Cells;背景說明：該細(xì)胞是１９５５年由PuckTT,MarcusPI和CieciuraSJ建系的，含HPV-１８序列；角蛋白陽性；可用于與染色體突變、細(xì)胞營養(yǎng)、集落形成相關(guān)的哺乳動(dòng)物細(xì)胞的克隆分析。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Hi-five細(xì)胞、RPTEC/TERT 1細(xì)胞、HNTEC細(xì)胞

J-774 Cells;背景說明：巨噬細(xì)胞；BALB/c;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：IMR 90細(xì)胞、A9細(xì)胞、Rat-1細(xì)胞

University of Michigan-Renal Carcinoma-2 Cells;背景說明：腎透明細(xì)胞癌;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HT 1376.T細(xì)胞、OVCAR 433細(xì)胞、PANC 1005細(xì)胞

BT-474[BT474]人乳腺導(dǎo)管癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

hTERT-HME1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：A-673細(xì)胞、COLO 320 HSR細(xì)胞、NL-20細(xì)胞

P-19 Cells;背景說明：P１９細(xì)胞株是從C３H/He小鼠中誘導(dǎo)的惡性畸胎瘤中建立的。該細(xì)胞在含有０.１mM的培養(yǎng)基中可高效率地克隆。該細(xì)胞具有多能性，在５００nM維A酸誘導(dǎo)下可以分化成神經(jīng)和神經(jīng)膠質(zhì)樣細(xì)胞；在０.５%~１.０%二甲亞砜(DMSO)存在下，分化形成心臟和骨骼肌樣細(xì)胞，但不形成神經(jīng)或神經(jīng)膠質(zhì)樣細(xì)胞；在DMSO和維A酸同時(shí)存在時(shí)，細(xì)胞的分化與只有維A酸一樣。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：HPAF/CD18細(xì)胞、184A1細(xì)胞、Spodoptera frugiperda clone 9細(xì)胞

GM05384 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：RWPE1細(xì)胞、MCF10-A細(xì)胞、SW403細(xì)胞

SK-N-BE(2)-M17 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MAntle cell VERona-1細(xì)胞、SNU398細(xì)胞、YH細(xì)胞

OV90 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代，３-４天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：McG-1細(xì)胞、CHP 212細(xì)胞、rUCMSCs細(xì)胞

H-2030 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３-１：４傳代；每周換液２-３次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：OCIAML4細(xì)胞、COLO-680N細(xì)胞、Helacyton gartleri細(xì)胞

KYSE 510 Cells;背景說明：食管鱗癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MCA-38細(xì)胞、HMrSV5細(xì)胞、JIMT1細(xì)胞

CAL-62 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SUSM1細(xì)胞、MGHU1細(xì)胞、QBC-939細(xì)胞

BayGenomics ES cell line LST053 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line XC295 Cells(提供STR鑒定圖譜)

CPTC-CDH1-3 Cells(提供STR鑒定圖譜)

MANSMA7-1B12 Cells(提供STR鑒定圖譜)

SC-3 A1b Cells(提供STR鑒定圖譜)

HP-mock (neo/puro) Cells(提供STR鑒定圖譜)

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Riaz M., van Jaarsveld M.T.M., Hollestelle A., Prager-van der Smissen W.J.C., Heine A.A.J., Boersma A.W.M., Liu J.-J., Helmijr J.C.A., Ozturk B., Smid M., Wiemer E.A.C., Foekens J.A., Martens J.W.M.

miRNA expression profiling of 51 human breast cancer cell lines reveals subtype and driver mutation-specific miRNAs.

Breast Cancer Res. 15:R33.1-R33.17(2013)

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Lu Y.-H., Soong T.D., Elemento O.

A novel approach for characterizing microsatellite instability in cancer cells.

PLoS ONE 8:E63056-E63056(2013)

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Timmerman L.A., Holton T., Yuneva M., Louie R.J., Padro M., Daemen A., Hu M., Chan D.A., Ethier S.P., van 't Veer L.J., Polyak K., McCormick F., Gray J.W.

Glutamine sensitivity analysis identifies the xCT antiporter as a common triple-negative breast tumor therapeutic target.

Cancer Cell 24:450-465(2013)

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Prat A., Karginova O., Parker J.S., Fan C., He X.-P., Bixby L.M., Harrell J.C., Roman E., Adamo B., Troester M.A., Perou C.M.

Characterization of cell lines derived from breast cancers and normal mammary tissues for the study of the intrinsic molecular subtypes.

Breast Cancer Res. Treat. 142:237-255(2013)

PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110; PMCID=PMC3937590

Daemen A., Griffith O.L., Heiser L.M., Wang N.J., Enache O.M., Sanborn Z., Pepin F., Durinck S., Korkola J.E., Griffith M., Hur J.S., Huh N., Chung J., Cope L., Fackler M.J., Umbricht C.B., Sukumar S., Seth P., Sume V.P., Jakkula L.R., Lu Y.-L., Mills G.B., Cho R.J., Collisson E.A., van 't Veer L.J., Spellman P.T., Gray J.W.

Modeling precision treatment of breast cancer.

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