香葉木素|T1704|TargetMol

價(jià)格	￥129	￥169	￥253
包裝	5mg	10mg	25mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-12-02

QQ交談微信洽談

產(chǎn)品詳情

中文名稱:香葉木素	英文名稱:Diosmetin
CAS:520-34-3	品牌: TargetMol
產(chǎn)地: 美國	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 99.84%	產(chǎn)品類別: 抑制劑
貨號(hào): T1704

Product Introduction

Bioactivity

名稱	Diosmetin
描述	Diosmetin (Luteolin 4-methyl ether) has been found to act as a weak TrkB receptor agonist.
細(xì)胞實(shí)驗(yàn)	Diosmetin is dissolved in DMSO which is maintained at a constant concentration in control samples (2%). HepG2 cells are maintained in a humidified atmosphere of 5% CO2 at 37°C, and cultured in RPMI-1640 medium supplemented with 10% (v/v) fetal bovine serum, 100 U/mL penicillin and 100 U/mL streptomycin. HepG2 cell density is adjusted to 2×104 cells/100 μL, and the cells are seeded into 96-well plates and placed in an incubator overnight (37°C in 5% CO2) to allow for attachment and recovery. MTT analyses are performed. Briefly, cells are pretreated with 5, 10, 15 and 20 μg/mL diosmetin for 24 h. A total of 20 μL MTT solution (5 mg/mL in PBS) solution is transferred to each well to yield a final 120 μL/well and to separate wells a total of 10 μL CCK8 (5 mg/mL in PBS) is transferred. The plates are incubated for 4 h at 37°C in 5% CO2 and the absorbance is recorded at wavelengths of 595 nm and 450 nm, respectively. The half maximal inhibitory concentration (IC50) of diosmetin is calculated[1].
激酶實(shí)驗(yàn)	Topoisomerase I Assay: One unit (the minimum amount for full relaxation of 0.5 μg SV40 DNA under the conditions of this study) of topoisomerase I, 0.5 μL of the test compounds, and 0.5μg SV40 DNA are added sequentially to the reaction buffer, which is composed of 25 mM Tris-HCl (pH 7.5), 50 mM KC1, 5 mM MgCl2, 0.25 mM EDTA disodium salt, 0.25 mM dithiothreitol, 15μg /mL bovine serum albumin, and 5% glycerol. Then, the reaction mixture (50 μL) is incubated for 10 min at 37 °C, and the reaction is terminated by treatment with 7.5 μL of a solution consisting of 1% sodium dodecyl sulfate, 20 mM EDTA disodium salt, and 0.5 mg/mL proteinase K for an additional 30 min at 37°C. The samples are mixed with 5 μL of the loading buffer containing 10 mM Na2HPO4, 31.3% sucrose, and 0.3% bromophenol blue. Relaxed (form Ir) DNA is separated from supercoiled (form I) and nicked (form II) DNA by electrophoresis on 0.8% agarose gel at 50 mA and 20 V for 17 h in the presence of 2 μg/mL chloroquine, 10 mM EDTA, 30 mM NaH2PO4, and 36 Mm Tris-HCl (pH 7.8). After electrophoresis, the gel is stained with 0.05% ethidium bromide and photographed with UV light (302 nm). The amount of DNA is quantified using a densitometer.
體內(nèi)活性	在首次注射藍(lán)銅膽素后的6小時(shí)、9小時(shí)及12小時(shí)，通過生化和形態(tài)學(xué)方法評估急性胰腺炎的嚴(yán)重程度。預(yù)處理Diosmetin顯著降低了血清淀粉酶和脂肪酶水平，抑制腫瘤壞死因子(TNF)-α、白細(xì)胞介素(IL)-1β和IL-6的分泌，抑制髓過氧化物酶(MPO)活性和胰蛋白酶原激活肽(TAP)水平，誘導(dǎo)一氧化氮合酶(iNOS)的表達(dá)，誘導(dǎo)藍(lán)銅膽素誘導(dǎo)的AP中核因子(NF)-κB激活。這項(xiàng)研究顯示，Diosmetin的給藥對小鼠藍(lán)銅膽素誘導(dǎo)的AP進(jìn)程有益效果。因此，Diosmetin可能成為未來臨床試驗(yàn)中治療AP的新療法。
存儲(chǔ)條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : < 1 mg/mL (insoluble or slightly soluble) DMSO : 18.33 mg/mL (61.06 mM), Sonication is recommended. Ethanol : < 1 mg/mL (insoluble or slightly soluble)
關(guān)鍵字	Inhibitor \| Diosmetin \| Cytochrome P450 \| CYPs \| inhibit
相關(guān)產(chǎn)品	Naringin \| 1-Aminobenzotriazole \| Amitriptyline hydrochloride \| Gemfibrozil \| Fenofibrate \| Tauroursodeoxycholate \| Methoxsalen \| Naringenin \| Apigenin \| 1-Ethynylnaphthalene \| Tebuconazole \| Diflubenzuron
相關(guān)庫	經(jīng)典已知活性庫 \| 已知活性化合物庫 \| 苗藥化合物庫 \| 黃酮類天然產(chǎn)物庫 \| 激酶抑制劑庫 \| 抗癌天然產(chǎn)物庫 \| 中藥單體化合物庫 \| 天然產(chǎn)物庫 \| 煙草單體化合物庫 \| 酪氨酸激酶分子庫

關(guān)鍵字：香葉木素|||Luteolin 4-methyl ether|TargetMol

公司簡介

上海陶術(shù)生物科技有限公司為美國Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國波士頓、德國慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實(shí)體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷售到個(gè)性化定制合成；從對明確靶點(diǎn)的分子篩選到對明確分子的多靶點(diǎn)篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過在中國市場五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過五百家學(xué)校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊資本	566.2651萬人民幣
員工人數(shù)	100-500人	年?duì)I業(yè)額	￥ 1億以上
主營行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營模式	貿(mào)易,試劑,定制,服務(wù)

TargetMol中國（陶術(shù)生物）

VIP 12年

公司成立：12年
注冊資本：566.2651萬人民幣
企業(yè)類型：有限責(zé)任公司(自然人投資或控股)
主營產(chǎn)品：小分子抑制劑，藥物篩選化合物庫，天然產(chǎn)物，活性分子化合物等
公司地址：上海市閘北區(qū)江場三路28號(hào)4樓

進(jìn)入店鋪

詢盤

店內(nèi)推薦

上海陶素獨(dú)家代理chemdiv成藥庫

￥100000

雷帕霉素

￥286.00

雷帕霉素

￥396