Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag? (Detergent)
分子別名（Synonym）
GPRC5D,G-protein coupled receptor family C group 5 member D
表達區(qū)間及表達系統(tǒng)（Source）
Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag (GPD-H82D5) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Cys 300 (Accession # Q9NZD1-2 ).
Predicted N-terminus: Asp
蛋白結構（Molecular Characterization）
This protein carries a Flag tag at the N-terminus, followed by a polyhistidine tag and an Avi tag (Avitag?) at the C-terminus. The protein has a calculated MW of 40.1 kDa.
標記（Labeling）
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標記度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>90% as determined by SDS-PAGE.
制劑（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
運輸（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存儲（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt;
-70°C for 3 months under sterile conditions.
背景（Background）
G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N terminal extracellular region. Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscle and ovary.
關鍵字： GPRC5D;GPRC5D蛋白;GPRC5D重組蛋白;ACRO;百普賽斯;
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