Mouse GPRC5D Protein, Flag,His Tag (Detergent)

分子別名（Synonym）

GPRC5D,G-protein coupled receptor family C group 5 member D

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse GPRC5D Protein, Flag,His Tag (Detergent) (GPD-M52D3) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Cys 300 (Accession # Q9JIL6-2).

Predicted N-terminus: Asp

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries flag tag at the N-terminus and polyhistidine tag at the C-terminus.

The protein has a calculated MW of 38.3 kDa. The protein migrates as 33-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>80% as determined by SDS-PAGE.

制劑（Formulation）

This product is not suitable for cell based experiments due to cytotoxicity of DDM.

DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.

DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.

If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

運(yùn)輸（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存儲（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景（Background）

G蛋白偶聯(lián)受體家族C組5成員D（GPRC5D）是一種維甲酸誘導(dǎo)的40kDa蛋白，具有七個(gè)跨膜片段和一個(gè)短的N端細(xì)胞外區(qū)。在外圍系統(tǒng)中廣泛表達(dá)。表達(dá)模式在胰腺中較高，在腎臟、小腸、脾臟和睪丸中中等，在肺、結(jié)腸、白細(xì)胞、前列腺和胸腺中較低，在腦、心臟、肝臟、胎盤、骨骼肌和卵巢中未檢測到。