Biotinylated Mouse Carbonic Anhydrase XII Protein, His,Avitag? (active enzyme, MALS verified)
分子別名(Synonym)
Carbonic Anhydrase XII,CA XII,Car12,CA-XII,Carbonate dehydratase XII
表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)
Biotinylated Mouse Carbonic Anhydrase XII Protein, His,Avitag (CAI-M82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Ser 301 (Accession # Q8CI85-1).
Predicted N-terminus: Ala 25
蛋白結(jié)構(gòu)(Molecular Characterization)
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This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.0 kDa. The protein migrates as 42-47 kDa under reducing (R) condition (SDS-PAGE).
標(biāo)記(Labeling)
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標(biāo)記度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, PH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
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背景(Background)
該基因編碼α-碳酸酐酶家族的一個(gè)膜結(jié)合成員,該酶催化二氧化碳可逆水合為碳酸氫鹽。這些蛋白質(zhì)參與各種生物過程,包括呼吸、鈣化、酸堿平衡、骨吸收以及房水、腦脊液、唾液和胃酸的形成。選擇性剪接導(dǎo)致多種轉(zhuǎn)錄變體。
關(guān)鍵字: CA XII;CA XII蛋白;CA XII重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。